In vitro clonal propagation of Clerodendrum serratum (Linn.) Moon (barangi): a rare and threatened medicinal plant

Sharma, Meena; K., S.; Purshottam, D. K.; Jain, M.; Chakrabarty, Debasis; Awasthi, Abhishek Kumar; Nair, K. S. S.; Sharma, Ashok Kumar

doi:10.1007/s11738-008-0245-4

Cited by 25 publications

(13 citation statements)

References 16 publications

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“…role of tryptophan in enhancing vegetative growth by foliar application has been reported in in vivo studies also in Ocimum basilicum [16], Pelargonium graveolens [17], and Philodendron erubescens [18]. The effectiveness of AdS for improved multiplication of shoots is also reported by many workers [19][20][21]. In the aforesaid treatment, sustained rate of shoot proliferation along with normal growth of regenerated shoots has been maintained for the last 5 years, observed so far (Fig.…”

Section: Resultssupporting

confidence: 59%

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Kumar

Sharma

Jain

et al. 2010

Appl Biochem Biotechnol

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An efficient in vitro process for rapid production of cloned plants of Uraria picta has been developed employing nodal stem segments taken from field-grown plants. Explants showed bud-break followed by regeneration of shoots with restricted growth within 12 days on modified Murashige and Skoog's medium supplemented with 0.25 mg l(-1) each of 6-benzylaminopurine and indole-3-acetic acid and 25 mg l(-1) adenine sulfate. Normal growth of shoots with good proliferation rate was achieved by reducing the concentrations of 6-benzylaminopurine and indole-3-acetic acid to 0.1 mg l(-1) each and incorporating 0.5 mg l(-1) gibberellic acid in the medium in which, on an average, 19.6 shoots per explant were produced. Further, during successive subcultures, increased concentrations of adenine sulfate (50 mg l(-l)) and gibberellic acid (2 mg l(-l)) along with the addition of 20 mg l(-l) DL: -tryptophan were found conducive to control the problem of necrosis of shoots. In this treatment, several "crops" of shoots were obtained from single culture by repeated subculturing of basal portion of stalk in long-term. Isolated shoots rooted 100% in 0.25 mg l(-1) indole-3-butyric acid. In vitro-raised plants after hardening in inorganic salt solution grew normally in soil and came to flowering. Genetic fidelity of in vitro-raised plants was ascertained by rapid amplified polymorphic DNA (RAPD) markers. Also, quantitative estimation of two isoflavonones in their root extracts further confirmed true-to-type nature of plantlets.

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Section: Resultssupporting

confidence: 59%

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Kumar

Sharma

Jain

et al. 2010

Appl Biochem Biotechnol

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“…There are a number of reports in the literature which report similar results for detection of genetic fidelity using RAPD/ ISSR markers in different crops such as Dioscorea bulbifera (Narula et al 2007), Swertia chirayita (Joshi and Dhawan 2007), Saccharum officinarum (Tawar et al 2008), Clerodendrum serratum (Sharma et al 2009), Dendrocalamus hamiltonii (Agnihotri et al 2009), Capparis deciduas (Tyagi et al 2010), and Cymbopogon martini (Bhattacharya et al 2010). Considering the importance of genetic stability in germplasm conservation programme, our protocol appears to be highly effective as it does not allow induction of variations and to the best of our knowledge it is the first report on genetic fidelity testing in micropropagated plants of jojoba.…”

Section: Resultsmentioning

confidence: 82%

Assessment of genetic fidelity of micropropagated plants of Simmondsia chinensis (Link) Schneider using RAPD and ISSR markers

et al. 2011

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RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeat) markers were screened to test the genetic integrity of jojoba (Simmondsia chinensis) plants multiplied through axillary bud multiplication from nodal segments. The in vitro raised plantlets were maintained for up to 12 in vitro subcultures. During the study a total of 48 (32 RAPD and 16 ISSR) primers were screened, out of which 24 RAPD and 13 ISSR primers produced a total of 191 (126 RAPD and 65 ISSR) clear, distinct and reproducible amplicons. The amplified products were monomorphic across all the selected micropropagated plants and were similar to the mother plant. The micropropagation protocol developed by our group for rapid in vitro multiplication is appropriate for clonal propagation of jojoba. The outcome supports the fact that axillary bud multiplication can also be used as one of the safest modes for the production of true-to-type plants.

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“…The fact that the addition of auxins did not increase the number of roots suggests that the in vitro culture of Bouchea fluminensis plants during the rooting phase should be performed in the absence of these growth regulators, which, in addition to reducing costs, would prevent the formation of calli at the base of the cuttings. However, rooting has been shown to be stimulated only in culture media supplemented with auxins for Vitex agnus-castus (Balaraju et al 2008), Clerodendrum serratum (Sharma et al 2009) …”

Section: In Vitro Rootingmentioning

confidence: 99%

An efficient system for in vitro propagation of Bouchea fluminensis (Vell.) Mold. (Verbenaceae)

et al. 2014

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This study aimed to establish and propagate in vitro plants of Bouchea fluminensis, a medicinal species known in Brazil as gervão-falso ("false verbena"), evaluating the influences of different growth regulators on in vitro multiplication and rooting stages, as well as examining ex vitro acclimatization of rooted plants. Explants were established on Murashige and Skoog medium at half strength of salts and vitamins without growth regulators. For multiplication, the explants were subjected to combinations of 6-benzyladenine (BA; 0, 2.5, 5.0 and 7.5 μM) and α-naphthalene-acetic acid (NAA; 0, 0.2, 0.4 and 0.6 μM). The medium found to induce the greatest number of shoot was that containing 5 μM of BA (NAA-free). For rooting, we evaluated three auxins (NAA, indole-3-acetic acid and indole-3-butyric acid; 0.1, 0.2, 0.3 and 0.4 μM), as well as a control. No differences were observed between the control and the other treatments. The auxin-free medium was deemed the most suitable, because it ensures the lowest cost in the micropropagation procedures. We obtained 100% survival of the acclimatized seedlings, and the plants showed normal vegetative and reproductive development, suggesting that the micropropagation did not alter the biological cycle of this species. The results show the importance and potential of micropropagation for biodiversity conservation of Bouchea fluminensis.

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In vitro clonal propagation of Clerodendrum serratum (Linn.) Moon (barangi): a rare and threatened medicinal plant

Cited by 25 publications

References 16 publications

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Assessment of genetic fidelity of micropropagated plants of Simmondsia chinensis (Link) Schneider using RAPD and ISSR markers

An efficient system for in vitro propagation of Bouchea fluminensis (Vell.) Mold. (Verbenaceae)

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