In Vitro Clonal Propagation of Nardostachys jatamansi: A Traditional Himalayan Medicinal Plant

Abstract

Plant tissue culture method has an impressive technique for Investigation and Explains basic and applied problems in plant biotechnology field. Micropropagation has played a vital role in the rapid multiplication of many plants species. The nodal explants and shoot tip of N. jatamansi inoculated in MS medium (Murashige and Skoog) contain different types concentrations of PGRs (Phytohormones) at various frequencies for the optimization of growth quality for shoot bud Induction, shoot proliferation and micro rooting in plant. The perfect shoot induction takes place in the concentration of BAP + IBA (2.0 mg/l +1.5 mg/1) multiplication of nodal explants and shoot tip in the combination lower concentration of BAP and KN (2.0 mg/1+1.5 mg/1) This combination proved best for multiple shoot formation. Half strength (1/2) of the MS medium containing NAA and BAP (1.5 mg/1+1.0 mg/1) in combination was most useful for rooting in plant. Well developed rooted micro shoots were smoothly removed for the culture flask and dipped in 70% ethanol for 2 min and then washed with running tap water for 5-10 min to remove media for the root and transferred to small plastic cups carry cocopeat, garden soil and sand (2:2:1) and produce healthy growth in ex-vitro conditions.

An Assessment of in vitro Propagation and Medicinal Properties of Datura stramonium (Dhatura)

“…For hardening prepare a pot containing garden soil, farmyard manure, and vermiculite free from any pest or infectious micro-organism, transfer the regenerated plant to the pot and cover it with plastic film to maintain humidity for the fresh plantlets. Around 10-12 days transfer the plants which are now soil established in the glass house and after that transfer to the larger area or pots filled with mixed soil and sand in equal proportion under greenhouse conditions (Gaurav, et al, 2015(Gaurav, et al, , 2016(Gaurav, et al, , 2018Gaurav and Kumar, 2019;Bhoora, et al, 2015;Pant, et al, 2020Pant, et al, , 2021Pant, et al, , 2022Sharma et al, 2022;Saini et al, 2021Saini et al, , 2022Verma et al, 2021;Rawat et al, 2021).…”

A Review on Endangered Medicinal Plant Nardostachys jatamansi: An Important Himalayan Herb

“…Jatamansi or its oil helps to prevent hair loss. It also encourages hair development and eliminates dryness (Pant et al, 2020) 8. Antidepressant: Because jatamansi extract has antidepressant properties, it may be good for persons who are depressed due to sleep disorders (Sahu et al, 2016).…”

“…Jatamansi is a perennial, aromatic, herbaceous plant with a height of 10-60 cm. Rhizome of the plant is small, grey, woody and coated with the reddish-brown, tufted, fibrous remains of dead leaves' petioles (Pant et al, 2021a). The leaves of the plant are rosette-shaped, lanceolate (Naya et al, 1988).…”

Effect of Growth Regulators on Direct Clonal Propagation and Analysis of Total Phenolic Content of Wild and Propagated Mucuna pruriens

“…The initiated shoots were permitted to develop for 20 days. Toward the finish of 20 days period, the explants creating the most extreme number of numerous shoots were refined on MS+BAP (30-40mg/l) for 30 days additional for the creation of a more prominent number of shoots (Saini et al, 2021;Gaurav et al, 2018;Pant et al, 2021).…”