In Vitro Clonal Propagation Through Bud Culture of Hemidesmus indicus (L) R Br: An Important Medicinal Herb

Saha, Soumen; Mukhopadhyay, Madhumita; Mukhopadhyay, Sandip

doi:10.1007/bf03263162

Cited by 14 publications

(4 citation statements)

References 6 publications

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“…Similarly, in other medicinal plants like Eclipta alba (Baskaran and Jayabalan, 2005), Vanilla planifolia (Abebe et al, 2009), Pogostemon cablin (Swamy et al, 2010) and Enicostema axillare (Loganathan and Bai, 2014) BA with Kn induced optimum shoots from in vitro nodes. Moreover, the potency of in vitro nodes to regenerate shoots was less as compared to in vivo nodal explants of the same plant (Patnaik and Debata, 1996;Sreekumar et al, 2000;Misra et al, 2003;Saha et al, 2003), it is also in contradiction to the earlier report which states that the potency of in vivo and in vitro nodes is the same (Sreekumar et al, 2000). But this study is in continuation of previous work in which from single node around 11 shoots were formed within eight weeks, each shoot had an average 7 nodes .…”

Section: Shoot Regenerationmentioning

confidence: 88%

“…Nevertheless, regeneration through leaf explant is known to induce soma clonal variants and hence nodes are best suitable explants when the aim is to produce true-to-type plants (Pathak and Joshi, 2018). There are reports on H. indicus regeneration using nodal explants (Patnaik and Debata, 1996;Sreekumar et al, 2000;Misra et al, 2003;Saha et al, 2003;Shekhawat and Manokari, 2016;. However, the plant is a slow growing and sparingly branched in wild (Sreekumar et al, 2000) and hence use of nodal explant for plant regeneration may increase the threat on its population.…”

Section: Introductionmentioning

confidence: 99%

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Regeneration of Hemidesmus indicus (L.) R. Br. using in vitro nodes: an alternative method for efficient multiplication of shoots

Pathak

Joshi

2021

Not Sci Biol

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In vivo nodes of Hemidesmus indicus (L.) R. Br. induced healthy multiple shoots with branching in our earlier studies and thus in the present study, potency of in vitro nodes to regenerate shoots was evaluated. In vitro nodes were excised from eight-week-old shoots and placed in Murashige and Skoog’s (MS) medium fortified with sucrose (3%) and different concentrations of 6-benzyladenine (BA) and kinetin (Kn). After eight weeks, optimum of 5.42 ± 0.36 shoots with 100% response were regenerated in medium supplemented with BA (10 µM) and Kn (5 µM). These healthy shoots were placed in full, half and quarter strengths of liquid MS medium fortified with sucrose (1%) and α-naphthaleneacetic acid (NAA, 1-25 µM) for rooting. Among all the strengths of MS medium, full strength MS medium having 8 µM NAA formed maximum of 3.42 ± 0.55 roots (91.67% response) within four weeks. The protocol is in continuation with earlier study and it was confirmed that a single in vivo nodal explant can regenerate around 385 healthy elongated shoots within 4 months, which will help in mass-propagation of the species.

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Section: Shoot Regenerationmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Regeneration of Hemidesmus indicus (L.) R. Br. using in vitro nodes: an alternative method for efficient multiplication of shoots

Pathak

Joshi

2021

Not Sci Biol

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“…An unusual phenolic compound, 2-hydroxy-4-methoxybenzaldehyde (MBALD) that accumulates in the root organs of H. indicus, is mainly responsible for this sweet flavor/fragrance (Sreekumar et al, 1998;Sircar et al, 2007a). Several reports are now available on the in vitro propagation of this plant of economic importance (Sreekumar et al, 2000;Misra et al, 2003;Saha et al, 2003). However, nothing is known on the enzymatic route of this fragrant hydroxybenzoate formation or even its distribution in the root tissue.…”

Section: Introductionmentioning

confidence: 96%

Phenylalanine ammonia-lyase-mediated biosynthesis of 2-hydroxy-4-methoxybenzaldehyde in roots of Hemidesmus indicus

Chakraborty

Sircar

Mitra

2008

Journal of Plant Physiology

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“…Adventitious shoots, which arise directly from the nonmeristematic tissues of the explant, can provide a reliable method to get true-to-type plants, where as the adventitious shoots arise through caulogenesis has the advantage of creating useful somaclonal variations and in gene transfer technology. Micropropagation through adventtious shoots has been reported as an appropriate method of multiplication in many Asclepiadaceous plants (Prasad et al, 2004;Saha et al, 2003;Faisal and Anis, 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration through adventitious buds in Wattakaka volubilis, a rare medicinal plant

Chakradhar¹,

Pullaiah²

2014

Afr. J. Biotechnol.

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High frequency shoot regeneration from in vitro derived leaf explants of Wattakaka volubilis (L.f.) Stapf was achieved through callus mediated organogenesis. Organogenic calli were induced from 20 day old aseptic seedling explants on Murashige and Skoog medium fortified with various concentrations and combinations of plant growth regulators, benzylaminopurine (BAP), α-naphthaleneacetic acid (NAA), indole 3-butyric acid (IBA) and gibberellic acid (GA 3). A mean of 8.6 shoots developed from organogenic callus induced from a 2 x 2 cm leaf explants on MS medium with 3% sucrose having 5.37µM NAA in combination with 2.22 µM BAP with 60% induction capacity. Further development of adventitious shoots could be achieved by sub culturing the callus to the same medium with 4.40 µM BAP and 0.288 µM GA 3. Organogenesis could not be achieved from the calli of ex vitro derived leaf explants. The developed shoots were rooted on half-strength MS medium with 1% sucrose, 4.90 µM IBA and 0.93 µM kinetin at a frequency of 85%. Well rooted plantlets were then transplanted to vermiculite soil (3:1) mixture in polythene covered pots kept under culture room conditions. Approximately 60% plantlets survived and grew into whole plants.

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In Vitro Clonal Propagation Through Bud Culture of Hemidesmus indicus (L) R Br: An Important Medicinal Herb

Cited by 14 publications

References 6 publications

Regeneration of Hemidesmus indicus (L.) R. Br. using in vitro nodes: an alternative method for efficient multiplication of shoots

Regeneration of Hemidesmus indicus (L.) R. Br. using in vitro nodes: an alternative method for efficient multiplication of shoots

Phenylalanine ammonia-lyase-mediated biosynthesis of 2-hydroxy-4-methoxybenzaldehyde in roots of Hemidesmus indicus

In vitro regeneration through adventitious buds in Wattakaka volubilis, a rare medicinal plant

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