In vitro co-culture model of human monocyte-derived dendritic cells and T cells to evaluate the sensitization of dinitrochlorobenzene

Bao, Lei; Hao, Changfu; Wang, Juan; Guo, Feifei; Geng, Zhongfeng; Wang, Di; Zhao, Youliang; Li, Yiping; Yao, Wu

doi:10.1016/j.ecoenv.2021.112331

Cited by 5 publications

(4 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The experimental process was referred to in the previous article 1–4 . On the first day of the experiment, a 2 × 2 cm 2 section of hair on the back of the mice was removed.…”

Section: Methodsmentioning

confidence: 99%

“…The experimental process was referred to in the previous article. 1 , 2 , 3 , 4 On the first day of the experiment, a 2 × 2 cm 2 section of hair on the back of the mice was removed. On the second, third and fourth days of the experiment, 30 μl of a 0.50% DNCB solution was applied to the shaved areas of the mice once a day for induction, and the smeared area was 1 × 1 cm.…”

Section: Methodsmentioning

confidence: 99%

“…Although the mechanisms of ACD are not fully known, multiple studies reported that immune system abnormalities and the influence of allergens are involved in the progress of ACD 2 . Dinitrochlorobenzene (DNCB) is a classic allergen to which more than 95% of people are sensitive 3 . The establishment of ACD in guinea pigs and mice with DNCB has a decades‐long history 4–6 .…”

Section: Introductionmentioning

confidence: 99%

“… 2 Dinitrochlorobenzene (DNCB) is a classic allergen to which more than 95% of people are sensitive. 3 The establishment of ACD in guinea pigs and mice with DNCB has a decades‐long history. 4 , 5 , 6 There is a linear relationship between the intensity of ACD skin reaction and the dose of exposed antigen.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression of interleukin‐1β, interleukin‐4, interferon‐γ and tumour necrosis factor α in different tissue in a dinitrochlorobenzene‐induced ear swelling test in mice

Liu²

2022

Skin Research and Technology

View full text Add to dashboard Cite

Objective To study the expression of interleukin‐1β (IL‐1β), interleukin‐4 (IL‐4), interferon‐γ (IFN‐γ) and tumour necrosis factor α (TNF‐α) in different tissue in a dinitrochlorobenzene (DNCB)‐induced ear swelling test in mice and further evaluate the correlation between the cytokine expression in different tissues and the degree of ear swelling. Methods The mice were sensitised with a 0.50% DNCB solution on their back for 3 days. After 7 days, the thickness of their ears was measured and grouped. Different concentrations of the DNCB solution were challenged in the left ear of each group of mice, and the right ear was used as the control. The thickness of both ears was measured every 24 h, and the mice were sacrificed 72 h after the challenge. The expressions of IL‐1β, IL‐4, IFN‐γ and TNF‐α in the mouse serum, lymph node and ear tissue were quantified by enzyme‐linked immunosorbent assay, respectively. Results There was a linear positive correlation between the swelling index of the mouse lateral ear and the challenge concentration of DNCB (r = 0.96, p < 0.01). The high expression of IL‐1β and IL‐4 in the lateral ear tissue of the mice was positively correlated with the ear swelling index 48 h after the challenge. The correlation coefficient was 0.78 (p < 0.01). Furthermore, IFN‐γ and TNF‐α had no significant correlation with the ear swelling index 48 h after the challenge. Conclusion There is a correlation between the degree of ear swelling in mice and the concentration of DNCB and the expression of IL‐1β and IL‐4 in the lateral ear tissue. There is a sub‐clinical skin sensitivity state in contact allergy.

show abstract

“…The experimental process was referred to in the previous article 1–4 . On the first day of the experiment, a 2 × 2 cm 2 section of hair on the back of the mice was removed.…”

Section: Methodsmentioning

confidence: 99%