In Vitro Coculture Assays of Angiogenesis

Wei, Haoche; Sundararaman, Ananthalakshmy; Truelsen, Sarah Line Bring; Gurevich, David; Thastrup, Jacob; Mellor, Harry

doi:10.1007/978-1-0716-0916-3_4

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Organotypic Culture Of Human Ec and Hdf Allows Creating Biom...1

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2023

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Cited by 2 publications

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“…To overcome the limitation of hydrogel based systems, we have refined an existing organotypic co-culture assay (organotypic vasculo-/angio-genesis assay, OVAA) 18,19 to create biomimetic vascularised microtissues. Previous work has shown the value of the OVAA to create patent capillary networks using HUVECs and human dermal fibroblasts (HDF) in a two-week timeframe.…”

Section: Resultsmentioning

confidence: 99%

Continuously perfusable, customisable, and matrix-free vasculature on a chip platform

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Continuously perfusable, customisable, and matrix-free vasculature on a chip platform

et al. 2023

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“…To overcome the limitation of hydrogel based systems, we have refined an existing organotypic coculture assay (Organotypic Vasculo-/Angio-genesis Assay, OVAA) (Bishop, 1999;Wei et al, 2021) to create biomimetic vascularised microtissues. Previous work has shown the value of the OVAA to create patent capillary networks using HUVEC and human dermal fibroblast (HDF) in a two-week timeframe.…”

Section: Organotypic Culture Of Human Ec and Hdf Allows Creating Biom...mentioning

confidence: 99%

Continuously perfusable, customisable and matrix-free vasculature on a chip platform

Chesnais

Joel

Hue

et al. 2022

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Creating vascularised cellular environments in vitro is a current challenge in tissue engineering and a bottleneck towards developing functional stem cells-derived microtissues for regenerative medicine and basic investigations. Here we have developed a new workflow to manufacture Vasculature on Chip (VoC) systems efficiently, quickly, and inexpensively. We have employed 3D printing for fast-prototyping of bespoke VoC and coupled them with a refined organotypic culture system (OVAA) to grow patent capillaries in vitro using tissue-specific endothelial and stromal cells. Furthermore, we have designed and implemented a pocket-size flow driver to establish physiologic perfusive flow throughout our VoC-OVAA with minimal medium use and waste. Using our platform, we have created vascularised microtissues and perfused them at physiologic flow rates for extended times and observed flow-dependent remodelling. Overall, we present for the first time a scalable, and customisable system to grow vascularised and perfusable microtissues, a key initial step to grow mature and functional tissues in vitro. We envision that this technology will empower fast prototyping and validation of increasingly biomimetic in vitro systems including interconnected multi tissue systems.

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In Vitro Coculture Assays of Angiogenesis

Cited by 2 publications

References 9 publications

Continuously perfusable, customisable, and matrix-free vasculature on a chip platform

Continuously perfusable, customisable, and matrix-free vasculature on a chip platform

Continuously perfusable, customisable and matrix-free vasculature on a chip platform

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