In Vitro Colony Studies in 87 Patients with Acute Nonlymphoblastic Leukemia

Hörnsten, Per; Gahrton, Gösta; Sundman, B.; Wahrén, Britta

doi:10.1111/j.0954-6820.1982.tb01926.x

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…Our previous ,finding that the absence of peripheral leucocyte CSA is associated with poor prognosis (8,9) was confirmed by the present results. However, the poor prognosis within the group of patients with CSA in excess of 20 ( Table 6) was an unexpected finding.…”

Section: Discussionsupporting

confidence: 91%

“…The assay for CFU-C and peripheral leucocyte CSA was performed using what was essentially the original Pike and Robinson double-layer agar system (17,18); it has been described in detail previously (9). Cultures were scored after 10-14 d of incubation and the incidence of colonies (aggregates with > 20 cells) was recorded and expressed as the mean of 4 parallel plates.…”

Section: Culture Techniquesmentioning

confidence: 99%

“…Monosomy 7 or deletion of the long arm of chromosome 7 (7q-) was found in 4 patients (nos. 9,13,15,22) (Table 3 ) . They were all more than 60-years-old.…”

Section: Cytogeneticsmentioning

confidence: 99%

“…Likewise, in vitro clonal assay systems for the analysis of haematopoietic progenitor cells (CFU-C) and their regulators have been used increasingly as a prognostically valuable method in human leukaemias (4-7). In previous studies, we found colony-stimulating activity (CSA) of peripheral leucocytes to be an important prognostic factor in ANLL (8,9).…”

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confidence: 98%

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Prognostic implications of in vitro colony studies and clonal chromosomal aberrations in adults with acute leukaemia

Hörnsten¹,

Lindquist²,

Gahrton³

1984

Scandinavian Journal of Haematology

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A comparison was made between in vitro colony culture parameters and chromosomal abnormalities in 44 adult patients with acute leukaemia, with special reference to prognosis. The presence of clonal chromosomal aberrations in some (AN) or all (AA) metaphases of bone marrow cells was a fairly strong indication of poor prognosis, while the absence of abnormal metaphases (NN) was a favourable prognostic sign. 6 of 7 patients in the NN group entered complete remission (CR), as did 8 of 20 in the AN + AA group and 8 of 17 in the group with random loss of chromosomes (NNA). The median survival time for AN + AA patients was 4 months, while for patients without clonal aberrations (NN + NNA) it was 9 months. Of the cultural parameters studied, colony‐stimulating activity (CSA) of peripheral leucocytes had the strongest correlation to prognosis. CSA > 1 was related to remission (P < 0.01) and longer survival (P < 0.05 at 4 months), while 8 patients with CSA ≤ 1 were all dead at 8 months. However, very high CSA values (> 20) indicate a poor prognosis. Although both chromosome analysis and CSA were of prognostic value, combining the 2 parameters did not improve the prognostic information, mainly because of covariation of NN and CSA > 1.

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Section: Discussionsupporting

confidence: 91%

Section: Culture Techniquesmentioning

confidence: 99%

“…Monosomy 7 or deletion of the long arm of chromosome 7 (7q-) was found in 4 patients (nos. 9,13,15,22) (Table 3 ) . They were all more than 60-years-old.…”

Section: Cytogeneticsmentioning

confidence: 99%

mentioning

confidence: 98%

See 2 more Smart Citations

Prognostic implications of in vitro colony studies and clonal chromosomal aberrations in adults with acute leukaemia

Hörnsten¹,

Lindquist²,

Gahrton³

1984

Scandinavian Journal of Haematology

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Marrow culture studies in adult acute myeloid leukemia at diagnosis and during complete remission

Shih

Chang

1985

Blut

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We used the human placental conditioned medium stimulated single layer agar culture technique to study the in vitro growth of marrow cells from 62 adult patients with acute myeloid leukemia (AML). Bone marrow cells were cultured from 50 patients at the time of initial diagnosis, 19 patients in early remission and 20 patients during their full complete remission. Marrow cultures from untreated patients exhibited heterogeneous growth patterns ranging from complete growth failure to excessive microcluster formation. We classified the growth patterns into 4 groups: (1) Gr I: normal growth, (2) Gr II: no growth, (3) Gr III: decreased growth, (4) Gr IV: excessive growth of microclusters. At presentation, none had Gr I growth; Gr II growth was observed in 23; Gr III in 14 and Gr IV in 13. A predominance of no growth were seen in M1 and M3 subtypes, while Gr IV growth was more commonly observed in M2 or M4 subtype. We were unable to correlate the culture findings with age or white cell count. The present results not only indicated that AML at diagnosis was characterized by abnormal granulopoiesis but also demonstrated that leukemic progenitor cells were heterogeneous with different capacities to express their proliferating potential in vitro. Except few with decreased growth, the growth characteristics generally returned to normal with successful remission induction. Both Gr II and Gr IV growth patterns were not observed either in early remission or during full complete remission.

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