In vitro comparison of complementary interactions between synthetic linear/branched oligo/poly-l-lysines and tissue plasminogen activator by means of high-performance monolithic-disk affinity chromatography

Влах, Е. Г.; Platonova, G. A.; Vlasov, G. P.; Kasper, Cornelia; Tappe, A; Kretzmer, G.; Tennikova, Tatiana

doi:10.1016/s0021-9673(03)00109-2

Cited by 24 publications

(12 citation statements)

References 22 publications

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“…4). One of them is direct attachment of an amino-bearing ligand such as a protein, peptide, or polyribonucleotide to the epoxy groups of the GMA-containing monolith [97,98]. However, the reaction between the amino group of the large ligand and epoxy groups is slow.…”

Section: Surface Modificationmentioning

confidence: 99%

Preparation of methacrylate monoliths

Влах

Tennikova²

2007

J of Separation Science

151

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Rigid macroporous polymers developed in the early 1990s are widely used as efficient stationary phases for all types of chromatographic separations. The main advantages of so-called monolithic supports are their high hydraulic permeability and the dominance of the convection over the diffusion mechanism of mass-exchange under dynamic conditions that allow the separation to be carried out at extremely high flow rates and, consequently, during very short operation times. Among other types of macroporous polymers, the methacrylate-based monolithic materials represent the most popular and successfully explored class of sorbents. This review is an attempt to collect together the contributions of different groups working in the area of monolith preparation. Examples of different methcrylate monomers and crosslinkers, as well as porogenic solvents, including polymer ones, used in monolith preparation are discussed.

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Section: Surface Modificationmentioning

confidence: 99%

Preparation of methacrylate monoliths

Влах

Tennikova²

2007

J of Separation Science

151

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“…Yamamoto et al [44] have also reported retention of oligonucleotides of various sizes under linear gradient conditions using anion exchange chromatography on thin monolithic columns. A short monolithic column was used to separate three linear lysine homologues and the support showed similar performance to conventional packed columns [45]. This shows that the thin monolithic columns have enough theoretical plates to fulfill the requirements of isocratic separation.…”

Section: Peptides and Oligonucleotidesmentioning

confidence: 80%

Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules

Roberts

Ongkudon

Forde

et al. 2009

J of Separation Science

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Polymethacrylate monoliths, specifically poly(glycidyl methacrylate-co-ethylene dimethacrylate) or poly(GMA-co-EDMA) monoliths, are a new generation of chromatographic supports and are significantly different from conventional particlebased adsorbents, membranes, and other monolithic supports for biomolecule purification. Similar to other monoliths, polymethacrylate monoliths possess large pores which allow convective flow of mobile phase and result in high flow rates at reduced pressure drop, unlike particulate supports. The simplicity of the adsorbent synthesis, pH resistance, and the ease and flexibility of tailoring their pore size to that of the target biomolecule are the key properties which differentiate polymethacrylate monoliths from other monoliths. Polymethacrylate monoliths are endowed with reactive epoxy groups for easy functionalization (with anion-exchange, hydrophobic, and affinity ligands) and high ligand retention. In this review, the structure and performance of polymethacrylate monoliths for chromatographic purification of biomolecules are evaluated and compared to those of other supports. The development and use of polymethacrylate monoliths for research applications have grown rapidly in recent times and have enabled the achievement of high through-put biomolecule purification on semi-preparative and preparative scales.

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“…Incubate the disk in a vial with immobilization solution at room temperature for a 20-24 h without any stirring (static immobilization method) [16,[18][19][20]26]. …”

Section: Methodsmentioning

confidence: 99%

“…Nowadays, this protein is produced by recombinant technique and need to be isolated from culture media. For isolation of t-PA the affinity chromatography based on monolithic sorbents containing monoclonal anti-t-PA antibodies, plasminogen or specific to t-PA short peptides, namely, Gly-Pro-Arg-Pro or Lys-Cys-Pro-Gly-Arg-Val, as specific ligands can be applied [18][19][20]. The selectivity of t-PA recovery with application of antibodies as ligands is higher than that found for the case of synthetic peptide ligands use.…”

Section: Analytical Scale Isolation Of Protein G From E Coli Cell Lymentioning

confidence: 99%

Affinity Chromatography of Proteins on Monolithic Columns

Влах

Platonova

Tennikova

2014

Methods in Molecular Biology

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At present, monolithic stationary phases, because of their morphology, are widely used for development and realization of fast dynamic and static processes based on mass transition between liquid and solid phases. These are liquid chromatography, solid phase synthesis, microarrays, flow-through enzyme reactors, etc. High-performance liquid chromatography on monoliths, including bioaffinity mode, represents a unique technique appropriate for fast and efficient separation of biological (macro)molecules of different sizes and shapes (proteins, nucleic acids, peptides), as well as such supramolecular systems as viruses.In this work, the examples of application of commercially available macroporous monoliths for modern affinity processing are presented. In particular, the original methods developed for efficient isolation and fractionation of monospecific antibodies from rabbit blood sera, the possibility of simultaneous affinity separation of protein G and serum albumin from human serum, the isolation of recombinant products, such as protein G and tissue plasminogen activator from E. coli cell lysate and Chinese Hamster Ovary cell culture supernatant, respectively, are described in detail. The suggested and realized multifunctional fractionation of polyclonal pools of antibodies by combination of several short monolithic columns (disks) with different affinity functionalities stacked in the same cartridge represents an original and practically valuable method that can be used in biotechnology.

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In vitro comparison of complementary interactions between synthetic linear/branched oligo/poly-l-lysines and tissue plasminogen activator by means of high-performance monolithic-disk affinity chromatography

Cited by 24 publications

References 22 publications

Preparation of methacrylate monoliths

Preparation of methacrylate monoliths

Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules

Affinity Chromatography of Proteins on Monolithic Columns

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