In Vitro Compatibility and Combined Efficacy of Entomopathogenic Nematodes with Abamectin and Imidacloprid Against the White Grub, Pentodon bispinosus Kust.

El-Ashry, Ramadan M.; Ramadan, M.

doi:10.21608/eajbsf.2021.145817

Cited by 5 publications

(2 citation statements)

References 45 publications

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“…In general the major limitations in adoption of microbial control are, an extended time to cause sufficient larval mortality relative to chemical insecticide, narrow host range, cost of production and susceptibility to environmental degradation. Microbial control agents have been found to be very effective when combined with lower concentrations of insecticides 13 . When two control agents work independently on the same target host and do not affect the toxicity of one another, their combined effects can be additive or synergistic 14 .…”

Section: Introductionmentioning

confidence: 99%

Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura (Fabricius)

Sarkhandia,

Sharma,

Mahajan

et al. 2023

Sci Rep

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The imprudent use of insecticides causes the development of resistance in insect pest populations, contamination of the environment, biological imbalance and human intoxication. The use of microbial pathogens combined with insecticides has been proposed as an alternative strategy for insect pest management. This IPM approach may offer effective ways to control pests, in addition to lowering the risk of chemical residues in the environment. Spodoptera litura (Fabricius) is a major pest of many crops like cotton, maize, tobacco, cauliflower, cabbage, and fodder crops globally. Here, we evaluated the combined effects of new chemistry insecticides (chlorantraniliprole and emamectin benzoate) and entomopathogenic bacterial strains, Shewanella sp. (SS4), Thauera sp. (M9) and Pseudomonas sp. (EN4) against S. litura larvae inducing additive and synergistic interactions under laboratory conditions. Both insecticides produced higher larval mortality when applied in combination with bacterial isolates having maximum mortality of 98 and 96% with LC50 of chlorantraniliprole and emamectin benzoate in combination with LC50 of Pseudomonas sp. (EN4) respectively. The lower concentration (LC20) of both insecticides also induced synergism when combined with the above bacterial isolates providing a valuable approach for the management of insect pests. The genotoxic effect of both the insecticides was also evaluated by conducting comet assays. The insecticide treatments induced significant DNA damage in larval hemocytes that further increased in combination treatments. Our results indicated that combined treatments could be a successful approach for managing S. litura while reducing the inappropriate overuse of insecticides.

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Section: Introductionmentioning

confidence: 99%

Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura (Fabricius)

Sarkhandia,

Sharma,

Mahajan

et al. 2023

Sci Rep

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“…Imidacloprid disturbs the nerve function of the grubs and becomes sluggish so that IJs of tested nematodes can easily infect them. Also, it was noticed that the degree of interaction in synergism varies with different species of nematodes (Koppenhofer et al, 2000, El-Ashry et al,2020and El-Ashry and Ramadan, 2021. Manzoor, 2012 tested the combined effect of imidacloprid and EPNs for the control of eastern subterranean termite, Reticulitermes flavipes.…”

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confidence: 99%

Joint Actions Between Entomopathogenic Nematodes and Abamectin for Controlling the Termites, Psammotermes hypostoma (Desn.), and Anacanthotermes ochraceus (Burm.)

Mostafa

Olfat

Hegab

et al. 2022

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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Compatibility of entomopathogenic nematodes with insecticides against the cabbage white butterfly, Pieris rapae L. (Lepidoptera: Pieridae)

Aioub

El-Ashry

Hashem

et al. 2021

Egypt J Biol Pest Control

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Background Compatibility of entomopathogenic nematodes (EPNs) with insecticides is a crucial mainstay of integrated pest management (IPM) programs. This study was designed to evaluate the joint action of EPN species and insecticides when employed to deter 3rd and 4th larval instars of cabbage white butterfly, Pieris rapae L. (Lepidoptera: Pieridae) under laboratory conditions. EPNs [Steinernema carpocapsae (All strain), S. feltiae (Filipjev), Heterorhabditis bacteriophora (HP88), and H. bacteriophora (Ar-4)], at concentrations of 50, 100, and 125 IJs/larva, were tested with 3 insecticides (lambda-cyhalothrin, emamectin benzoate, and indoxacarb) at LC25 and LC50 values. Additionally, expression profiles of 2 detoxification genes (CYP6AE120 and PrGSTs1) when the 4th instar larvae were treated by H. bacteriophora (HP88) and lambda-cyhalothrin were examined. Results Data indicated that statistically significant mortality of 2 larval instars of P. rapae was observed in vitro among EPN species and pesticide concentrations. At concentration of 50 IJs/larva, LT50 values were 2.385 and 3.92 days for S. carpocapsae (All strain) and H. bacteriophora(Ar-4), respectively, on 3rd instar larvae; also, these values were 3.506 and 3.107 days for S. feltiae and H. bacteriophora (Ar-4), respectively, on 4th instar larvae Lambda-cyhalothrin was the most toxic insecticide, followed by emamectin benzoate and indoxacarb at LC25 and LC50, respectively. An additive effect was observed between EPN species with LC25 and LC50 of the tested insecticides, except for lambda-cyhalothrin at LC50 with H. bacteriophora (Ar-4), and indoxacarb, with all EPNs showing antagonistic effects on mortality of 3rd instar larvae after 3 days post-treatment. The interaction between the tested pesticides at LC25 and LC50 and EPN species, showed an additive effect, excluding lambda-cyhalothrin at LC25 with S. carpocapsae (All strain) and LC25 of indoxacarb with H. bacteriophora (Ar-4), which showed potentiation effects. The interaction of S. feltiae (Filipjev) with tested insecticides at LC50 exhibited an antagonistic effect on the mortality of 4th instar P. rapae larvae after 3 days post-treatment. The expression of both CYP6AE120 and PrGSTs1 was significantly up-regulated with lambda-cyhalothrin, followed by H. bacteriophora (HP88) compared to control. Conclusions The findings suggested that combining EPNs and the pesticide concentrations can be a practical strategy for managing P. rapae and could pave the way to using new control technologies in protecting organic farm vegetables from lepidopteran pests.

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In Vitro Compatibility and Combined Efficacy of Entomopathogenic Nematodes with Abamectin and Imidacloprid Against the White Grub, Pentodon bispinosus Kust.

Abstract: http://eajbsf.journals.ekb.eg/ In Vitro Compatibility and Combined Efficacy of Entomopathogenic Nematodes with Abamectin and Imidacloprid Against the White Grub, Pentodon bispinosus Kust.

Cited by 5 publications

References 45 publications

Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura (Fabricius)

Synergistic and additive interactions of Shewanella sp., Pseudomonas sp. and Thauera sp. with chlorantraniliprole and emamectin benzoate for controlling Spodoptera litura (Fabricius)

Joint Actions Between Entomopathogenic Nematodes and Abamectin for Controlling the Termites, Psammotermes hypostoma (Desn.), and Anacanthotermes ochraceus (Burm.)

Compatibility of entomopathogenic nematodes with insecticides against the cabbage white butterfly, Pieris rapae L. (Lepidoptera: Pieridae)

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