In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

Gomes, Hugo Teixeira; Bartos, Patrícia Monah Cunha; Andrade, Maíra Teixeira de; Almeida, Raphael Ferreira; Lacerda, Luciana Florêncio de; Scherwinski‐Pereira, Jonny Everson

doi:10.1590/s0100-204x2017001200018

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…One can recommend that passage time of three months was the most suitable regarding the measured parameters and healthiness of cultures. Most researchers who worked on the micropropagation of Blackberry stated that period of 4 or 6 weeks as a passage time among subcultures (Fira et al, 2014, Gomes et al, 2017and Aly et al, 2022.…”

Section: Data In Tablementioning

confidence: 99%

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Micropropagation of Blackberry (Rubus fruticosus.) cv. Karaka Black

Samaan

Nasser

2022

Egyptian Journal of Horticulture

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T HIS work aimed to establish an in vitro protocol for Blackberry "Karaka Black" micropropagation. Stem node explants were experimented on three types of medium salts (WPM, MS and B5) in establishment stage. Also, three types of cytokinins were tested for multiplication i.e. BA (0.0, 0.4, 0.8 and 1.2 ppm), TDZ (0.0, 0.1, 0.5 and 1.0 ppm) and Kin (0.0, 1.0, 5.0 and 10.0 ppm). Two soil mixtures were used in acclimatization stage. Finally, the effect of passage time and number of subcultures on multiplication rate were investigated. The results indicated that WPM followed by MS medium gave the highest shoot length and leaf number. BA at 0.4 ppm, Kin at 5.0 ppm and TDZ at 0.1 ppm achieved the most preferable values for multiplication stage but the kinetin one gave the healthiest plantlets. The most microshoots got well-formed roots in multiplication phase. Consequently, rooting stage was not required. The multiplication rate slightly decreased after the 3 rd subculture and three months of culture date proved to be the most suitable passage time among subcultures. Peatmoss and sand mixture gave the highest plantlet quality during acclimatization.

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Section: Data In Tablementioning

confidence: 99%

“…Gomes et al, 2017, carried out five successive subcultures on four genotypes of Blackberry, the multiplication rate of each genotype showed a fluctuation among the different subcultures. Consequently, 'karaka Black' may have its own behavior.…”

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confidence: 99%

Micropropagation of Blackberry (Rubus fruticosus.) cv. Karaka Black

Samaan

Nasser

2022

Egyptian Journal of Horticulture

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“…Blackberry plants are successfully propagated by traditional methods such as layering and cuttings [ 8 ], ensuring the preservation of desirable agronomic characteristics [ 2 , 9 ]. However, these methods require a large plantation area, high labor demand, and intensive weed control [ 10 ]. To overcome the abovementioned limitations, the in vitro propagation (micropropagation) of blackberries has been successfully applied, and it now stands as the most common propagation method [ 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, these methods require a large plantation area, high labor demand, and intensive weed control [ 10 ]. To overcome the abovementioned limitations, the in vitro propagation (micropropagation) of blackberries has been successfully applied, and it now stands as the most common propagation method [ 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

The Influence of the Explant’s Type on the Performance of Synthetic Seeds of Blackberry (Rubus spp.)

Regni,

Micheli,

Facchin

et al. 2023

Plants

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In vitro propagation, also known as micropropagation, has become the most widely employed method for blackberry propagation, as it overcomes the limitations of the traditional asexual propagation methods (mainly layering and cutting). In this context, synthetic seed technology represents a strategy to enhance the productivity of in vitro propagation and facilitates the exchange of plant materials between laboratories, contributing to germplasm conservation. This study aimed to identify the most suitable vegetative propagule for the encapsulation of blackberry. To this end, uninodal microcuttings (nodes) and the base of clumps were used to produce synthetic seeds for the cultivars Thornfree and Chester. Forty-five days after sowing, viability (percentage of green propagules without browning or necrosis), regeneration (percentage of propagules that sprouted and rooted simultaneously), number of shoots produced, shoot length, number of roots produced, root length, and the fresh and dry weights of the plantlets were measured. The results demonstrated that both considered propagules allowed us to obtain satisfactory regeneration rates. However, plantlets originating from the encapsulated clump’s base had more shoots and roots, resulting in greater fresh and dry weights than the plantlets derived from encapsulated nodes. Therefore, for achieving more robust plantlets and enhancing overall procedural efficiency, we recommend using the base of clumps as a propagule for blackberry encapsulation.

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“…After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication [11].…”

Section: Introductionmentioning

confidence: 99%

The study of the consequences of preserving berry crops in vitro on the processes of subsequent clonal micropropagation

Knyazeva

2021

BIO Web Conf.

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As a result of studies, it was found that collection samples of berry crops in vitro can be maintained in viable condition on specially selected nutrient media for 12 months. The effect of mannitol concentration (0.45, 0.75 and 1.05%; sucrose control -3%) on the regenerative potential of microprobe of the studied berry crop varieties was estimated. When studying the effect of the reproduction cycle on the morphogenesis of meristemic cultures, it was found that the dynamics of changes in shoot length, number of leaves and shoots depended on the concentration of the active substance. In general, the majority of berry crops that were viable after storage of explants retained the ability to grow again, in many cases reaching or exceeding the control level.

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In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

Cited by 7 publications

References 9 publications

Micropropagation of Blackberry (Rubus fruticosus.) cv. Karaka Black

Micropropagation of Blackberry (Rubus fruticosus.) cv. Karaka Black

The Influence of the Explant’s Type on the Performance of Synthetic Seeds of Blackberry (Rubus spp.)

The study of the consequences of preserving berry crops in vitro on the processes of subsequent clonal micropropagation

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