In vitro conservation of Dendrobium germplasm

Abstract

Dendrobium is a large genus in the family Orchidaceae that exhibits vast diversity in floral characteristics, which is of considerable importance to orchid breeders, biotechnologists and collectors. Native species have high value as a result of their medicinal properties, while their hybrids are important as ornamental commodities, either as cut flowers or potted plants and are thus veritable industrial crops. Thus, preservation of Dendrobium germplasm is valuable for species conservation, breeding programs and the floriculture industry. Cryopreservation represents the only safe, efficient and cost-effective long-term storage option to facilitate the conservation of genetic resources of plant species. This review highlights 16 years of literature related to the preservation of Dendrobium germplasm and comprises the most comprehensive assessment of thorough studies performed to date, which shows reliable and reproducible results. Air-drying, encapsulation-dehydration, encapsulation-vitrification, vitrification and droplet-vitrification are the current cryopreservation methodologies that have been used to cryopreserve Dendrobium germplasm. Mature seeds, pollen, protoplasts, shoot primordia, protocorms and somatic embryos or protocorm-like bodies (PLBs) have been cryopreserved with different levels of success. Encapsulation-vitrification and encapsulation-dehydration are the most used protocol, while PLBs represent the main explant explored.

Asymbiotic in vitro seed propagation of Dendrobium

“…They also allow for the establishment of sterile in vitro cultures for the study of the genetics of flowering in orchids (Teixeira da Silva et al 2014c). Another strategy is to use seeds aseptically germinated in vitro, under optimized conditions, modeling natural ones.…”

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

“…3.1) Qian et al, 2014). Despite previous attempts to revive D. officinale, an urgent need for conserving wild D. officinale still exists (Li et al, 2018a;Liu et al, 2015;Teixeira da Silva et al, 2014b). To meet the demand for commercial production, new cultivars should be developed and methods for efficient propagation should be established (Ding et al, 2008).…”

Composition of Volatile Fraction from Inflorescences and Leaves of Dendrobium moschatum (Orchidaceae)

“…The inflorescence consists of 7 to 15 short lasting fragrant flowers, each one of 5-8 cm diameter [11,12]. Due to the wild over-collection of plants for traditional use, many Dendrobium species are, nowadays, target species for in situ, ex situ and in vitro conservation projects [13]. A protocol for rapid mass propagation of D. moschatum through stem disc culture has also been developed [14].…”