In vitro conservation of embryogenic cultures of date palm using osmotic mediated growth agents

Abstract

This study was carried out to investigate the effect of mannitol, sorbitol and sucrose as osmotic agents on in vitro conservation of embryogenic cultures of date palm (Phoenix dactylifera, L.) Bartamoda and Sakkoty cultivars. Embryogenic cultures was obtained using MS medium supplemented with 10 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 3 mg/l isopentenyl adenine (2iP). Among the three types of osmotic substances used for slow growth conservation, sucrose at all concentrations gave the highest percentage of survival with Sakkoty cultivar. However, addition of 40 g/l or 60 g/l mannitol and 20 g/l sorbitol showed the highest percentage of survival percentage with Bartamoda cultivar. The different sucrose concentrations caused higher numbers of germinated embryos of the two cultivars compared with mannitol or sorbitol. Also, the number of germinated embryos was increased with increasing the storage periods till the ninth month. Genetic stability was determined using random amplified polymorphic DNA (RAPD) analysis. There were no clear genetic differences between the two osmotic agents used for preservation. The preserved cultures of Sakkoty cultivar gave the high percent of similarity while Bartamoda cultivar gave low percent of similarity. From the obtained results we can recommend using 40 g/l mannitol or 20 g/l sorbitol for in vitro preservation of Bartamoda cultivar of date palm and 20 g/l of sucrose for Sakkoty cultivar.

In vitro conservation of Amburana cearensis (Fabaceae)

“…other studies have demonstrated that the presence of sorbitol and/or mannitol combined with sucrose in the conservation medium increased the survival percentage of various plant species (TAVAZZA et al, 2015;TAKUR et al, 2015;EL-BAHR et al, 2016;NASIRUDDIN AND ISLAM, 2018).…”

“…This reduction was greater at the end of the experiment (10 months), in the presence of higher concentrations of sucrose, sorbitol or mannitol, as observed in M8 and M12, with 0.0% and 10.0% survival, respectively. osmotic agents acted as growth retardants by causing osmotic stress to the material under conservation (EL-BAHR et al, 2016).…”

Development of Cryopreservation Technique for Meristems of Syringa vulgaris L. Cultivars

“…In our study, we used both these methods to induce tolerance to cryopreservation in plants: before the start of the step (ii), the donor-plants were hardened on the medium with 60 g•L −1 sucrose at 8 • C for two weeks, and the excited explants were hardened on the medium enriched with sucrose (273.84 g•L −1 ) at 0-2 • C for 48 h. High sucrose in medium significantly increases cold-tolerance [44,45]. Sucrose was also used as osmotic agent, which restricts the water availability to the explant and goes about a development retardant, when added to the medium [46,47].…”

In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

“…In turn, for the dry mass of the roots, the smallest averages were obtained in treatments M1, M2, M3, M4, M7, M10 and M12, with respective values of 47.64 mg, 70.63 mg, 78.38 mg, 43.79 mg, 73.34 mg, 56.50 mg and 39.23 mg (Figure 3f). The decrease in the dry mass of the aerial part and roots of P. pyramidalis with reduction of osmotic potential of the culture medium can possibly be explained by the action of the osmotic agents when added to the medium, by reducing the water potential and availability of water and nutrients in the medium, inducing slower growth (Huang et al, 2014;El-Bahr et al, 2016). Therefore, the reduction in the growth of the P. pyramidalis plants, reflected in the lower dry mass values, is an advantage for in vitro conservation, where the aim is to minimize the growth of the plants to increase the interval between subcultures.…”