In vitro conservation of Mandevilla moricandiana (Apocynaceae): short-term storage and encapsulation–dehydration of nodal segments

In vitro approaches for conservation of Asparagus racemosus Willd.

“…Moreover, the effect of these osmotic agents depends on the temperature, genotype, and culture conditions, as suggested by Marino et al (2010). Mannitol has often been found to have deleterious effects on the quality of stored cultures (Conner and Falloon 1993;Cordeiro et al 2014), in contrast to our findings. In our experiments, effective storage was achieved by incorporation of mannitol at low concentrations into the medium, whereas higher concentrations of osmoticum were deleterious to in vitro-stored cultures of A. racemosus.…”

“…Also, in other species such as potato (Solanum tuberosum: Sarkar and Naik 1998) and enset (Enset ventricosum: Negash et al 2001), the addition of mannitol to the storage medium resulted in improved survival of stored cultures. The sugaralcohol sorbitol has an osmotic potential similar to that of mannitol in solutions at equivalent molarity and has been employed as an osmoticum in many species (Cordeiro et al 2014). Fletcher (1994) incorporated 4% sorbitol in the conservation medium for storage of Asparagus officinalis L. up to 16 mo.…”

Encapsulation of Shoot Tips and Nodal Segments for in Vitro Storage of “Kober 5BB” Grapevine Rootstock

“…The encapsulation technique is used today in advanced procedures of cryopreservation such as encapsulation-dehydration and encapsulation-vitrification methods [4], obtaining very promising results for the long-term preservation of plant germplasm [5][6][7]. The encapsulation of shoot tips, axillary buds or nodal segments has been reported for various species [8][9][10][11][12][13], and slow growth storage has been applied as a method for medium-term conservation [14][15][16]. Slow growth storage involves limiting development by reducing temperature and/or light intensity, adding osmotic compounds such as mannitol or sucrose in the culture medium, and use of growth retardants [17].…”

Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

“…La variabilidad de respuestas mencionadas en las distintas especies con respecto al manitol y sorbitol, se pueden deber a que estos compuestos cumplen dos roles uno como agentes osmóticos, limitando la disponibilidad de agua y nutrientes lo cual resulta en un lento crecimiento (Gollagunta et al 2005;Rejšková et al 2007;Gopal & Chayhan 2010;Lata et al 2010;Nogueira et al 2014) o la muerte de los tejidos (Cordeiro et al 2014); y en otras especies que producen naturalmente manitol puede resultar que al inicio actúe como un agente osmótico con un efecto menor y luego como una fuente de carbono y energía debido a que se absorben lentamente y se metaboliza con facilidad, por tanto su función en estas especies como agente osmótico es a corto plazo . Esto ha sido observado en el cultivo de Olea europea con un significativo incremento del crecimiento y proliferación de brotes (García et al 2002;Conde et al 2011).…”