In vitro control of Colletotrichum lindemuthianum by Trichoderma spp. and in vivo with Alternative Products

Abstract

Beans (Phaseolus vulgaris) are extremely important because it is included daily in the diet of themajority of the Brazilian population. Several factors negatively affect the productivity of this crop, especially diseases.One of the main ones is anthracnose (Colletotrichum lindemuthianum), which can cause total production damage and depreciation of the final product. The study and development of new strategies for integrated anthracnose management can reduce the cost of production and consequently reduce environmental impacts. The objective of this work was to evaluate the efficiency of Trichoderma fungi in in vitro tests (antagonism, production of volatile and non-volatile compounds) for control of C. lindemuthianum and to evaluate the efficiency of endophytic fungi, salicylic acid, copper phosphite, acibenzolar-S-methyl (ASM) and fungicide for anthracnose control in greenhouse bean plants. Anthracnose was controlled in bean plants with the use of alternative products. The endophytic fungi Trichoderma viride and Trichoderma tomentosum inhibited C. lindemuthianum mycelial growth in the three in vitro tests. In greenhouse, T. viride, T. tomentosum, salicylic acid, ASM and fungicide were effective, but copper phosphite was not efficient in controlling anthracnose.

Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops

“…Gadaga et al (2017) and Hennipman et al (2019) reported lower anthracnose and angular spot severity with copper phosphite application on bean crops. However, Christmann et al (2019) and Assunção et al (2020) did not find the same result for anthracnose control in the same crop. )…”

Context-Dependent Effects of Trichoderma Seed Inoculation on Anthracnose Disease and Seed Yield of Bean (Phaseolus vulgaris): Ambient Conditions Override Cultivar-Specific Differences

“…Any preventative resistance induction can generate metabolic or other costs which might result in negative effects on seed yield under enemy-free conditions, or when other environmental factors than the particular disease limit seed yield [ 62 ]. Evidently, such costs are likely to pay off if they protect the plant from a yield-limiting disease [ 63 , 64 , 65 ]. Indeed, inoculation of FMA bean seeds with each of the Trichoderma strains had a negative effect on seed yield in the anthracnose-free plot, but not so in the presence of anthracnose.…”

“…To the best of our knowledge, only one study reported a delay of anthracnose symptoms in the leaves of P. vulgaris after seed inoculation with T. viride or T. tomentosum and thereby shows that—in principle— Trichoderma spp. can trigger systemic resistance to C. lindemuthianum [ 65 ].…”

Potential Bioinoculants for Sustainable Agriculture Prospected from Ferruginous Caves of the Iron Quadrangle/Brazil

“…Among the most used Cl control strategies are the use of resistant cultivars [107], the use of chemical controllers sprayed on the aerial part of the plant [108], seed treatment [109] and crop rotation [110]. Similarly, the use of biological control agents has been shown to be promising against Cl [111][112][113][114] and bacteria have played a prominent role in this approach [115,116].…”