In vitro controlled release of an anti-inflammatory from daily disposable therapeutic contact lenses under physiological ocular tear flow

Tieppo, Arianna; Pate, Kayla M.; Byrne, Mark E.

doi:10.1016/j.ejpb.2012.01.015

Cited by 90 publications

(62 citation statements)

References 42 publications

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“…7 Moreover, two important factors of the ocular environment, natural tear flow and the blinking reflex, are absent from the simple static vial model. The limitations of the conventional vial model have been recognized by researchers, and attempts have been made to create unique in vitro eye models simulating the ocular environment, by including a microfluidic tear replenishment component [20][21][22][23][24] and/or intermittent air exposure. 25,26 Not surprisingly, the results generated from these experiments are very different than those obtained with the conventional vial model, and may more closely resemble in vivo data.…”

Section: Discussionmentioning

confidence: 99%

“…25,26 Not surprisingly, the results generated from these experiments are very different than those obtained with the conventional vial model, and may more closely resemble in vivo data. [20][21][22][23][24][25] Thus, developing an intricate in vitro eye model to examine CLs will provide new insights on the interaction of lens materials with the ocular surface, and help facilitate the development of new materials and new applications for CLs in the coming decades.…”

Section: Discussionmentioning

confidence: 99%

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Development of an <em>In Vitro</em> Ocular Platform to Test Contact Lenses

Phan

Walther

Gao

et al. 2016

JoVE

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Currently, in vitro evaluations of contact lenses (CLs) for drug delivery are typically performed in large volume vials, 1-6 which fail to mimic physiological tear volumes. 7 The traditional model also lacks the natural tear flow component and the blinking reflex, both of which are defining factors of the ocular environment. The development of a novel model is described in this study, which consists of a unique 2-piece design, eyeball and eyelid piece, capable of mimicking physiological tear volume. The models are created from 3-D printed molds (Polytetrafluoroethylene or Teflon molds), which can be used to generate eye models from various polymers, such as polydimethylsiloxane (PDMS) and agar. Further modifications to the eye pieces, such as the integration of an explanted human or animal cornea or human corneal construct, will permit for more complex in vitro ocular studies. A commercial microfluidic syringe pump is integrated with the platform to emulate physiological tear secretion. Air exposure and mechanical wear are achieved using two mechanical actuators, of which one moves the eyelid piece laterally, and the other moves the eyeballeyepiece circularly. The model has been used to evaluate CLs for drug delivery and deposition of tear components on CLs. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of an <em>In Vitro</em> Ocular Platform to Test Contact Lenses

Phan

Walther

Gao

et al. 2016

JoVE

View full text Add to dashboard Cite

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“…In an effort to develop contact lenses capable of such use, a range of technologies have been applied, including molecular imprinting, an approach pioneered by Byrne et al [5,10]. This technology creates specific binding sites within the polymeric network that allow for increased drug loading and the release of compounds such as hyaluronic acid [10], diclofenac sodium [11] and, as stated, ketotifen fumarate [5] indicates the potential of this promising technology.…”

Section: Introductionmentioning

confidence: 97%

Synthesis and Characterization of Poly(2-hydroxyethylmethacrylate) Contact Lenses Containing Chitosan Nanoparticles as an Ocular Delivery System for Dexamethasone Sodium Phosphate

et al. 2016

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“…The desirability of designing release devices that more closely replicate in-eye conditions is now well recognised. Byrne and his co-workers [21,41] have made excellent progress in 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 12 this regard. The important effect of extraction volume in release studies is demonstrated in figure 8.…”

Section: Volume Of Extraction Mediamentioning

confidence: 99%

“…An additional but extremely important aspect of in vivo extraction that needs to be reflected in vitro models of ocular release is the effect of tear volume and tear turn over. The work of Byrne [21,41] has produced considerable advances in reduced volume in vitro devices. These parallel studies using ophthalmic dyes have aimed to link in vitro studies of three specific physic chemical properties to observed in-eye behaviour.…”

Section: In Vivo Release Studies: Preliminary Demonstration Of Ocularmentioning

confidence: 99%

Structural design of contact lens-based drug delivery systems; in vitro and in vivo studies of ocular triggering mechanisms

Mahomed

Wolffsohn

Tighe

2016

Contact Lens and Anterior Eye

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This study identifies and investigates the potential use of in-eye trigger mechanisms to supplement the widely available information on release of ophthalmic drugs from contact lenses under passive release conditions. Ophthalmic dyes and surrogates have been successfully employed to investigate how these factors can be drawn together to make a successful system. The storage of a drug-containing lens in a pH lower than that of the ocular environment can be used to establish an equilibrium that favours retention of the drug in the lens prior to ocular insertion. Although release under passive conditions does not result in complete dye elution, the use of mechanical agitation techniques which mimic the eyelid blink action in conjunction with ocular tear chemistry promotes further release. In this way differentiation between passive and triggered in vitro release characteristics can be established. Investigation of the role of individual tear proteins revealed significant differences in their ability to alter the equilibrium between matrix-held and eluate-held dye or drug. These individual experiments were then investigated in vivo using ophthalmic dyes. Complete elution was found to be achievable in-eye; this demonstrated the importance of that fraction of the drug retained under passive conditions and the triggering effect of in-eye conditions on the release process. Understanding both the structure-property relationship between drug and material and in-eye trigger mechanisms, using ophthalmic dyes as a surrogate, provides the basis of knowledge necessary to design ocular drug delivery vehicles for in-eye release in a controllable manner.

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In vitro controlled release of an anti-inflammatory from daily disposable therapeutic contact lenses under physiological ocular tear flow

Cited by 90 publications

References 42 publications

Development of an <em>In Vitro</em> Ocular Platform to Test Contact Lenses

Development of an <em>In Vitro</em> Ocular Platform to Test Contact Lenses

Synthesis and Characterization of Poly(2-hydroxyethylmethacrylate) Contact Lenses Containing Chitosan Nanoparticles as an Ocular Delivery System for Dexamethasone Sodium Phosphate

Structural design of contact lens-based drug delivery systems; in vitro and in vivo studies of ocular triggering mechanisms

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