In vitro corm production of Gladiolus dalenii and G. tristis

Abstract

The ability of the summer flowering Gladiolus dalenii Van Geel and the winter flowering G. tristis L. to form corms in vitro was investigated. G. dalenii spontaneously formed corms on a shoot induction medium consisting of the basal medium of Murashige & Skoog (1962) with up to 2.0mg1-1 benzyladenine (BA), 3% sucrose and solidified with 2 g l 1 Gelrite ®. The effect of different BA and sucrose concentrations as well as different temperatures on in vitro corm production of G. tristis was further investigated. The best production of shoots per explant was achieved on a medium containing 0.5 to 1.0mg1-1 BA, sucrose concentrations of 6 to 9% and cultured at 15°C. The best corm production was achieved at the same temperature and with the same medium with the exception that BA was omitted from the medium. To test the effect of the osmotic potential on the formation of shoots and corms, sucrose was substituted by mannitol at various concentrations. Sucrose proved to be essential for both shoot and corm production and the use of mannitol had no beneficial effect.

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

“…Due to the preformed apical meristems, stem discs are better choice for culture establishment and plants multiplied from such tissues are likely to be true-to-type. Both cytokinins BA and KIN promoted shoot multiplication, however, BA was found to be more effective than KIN (Table 2) in agreement with previous reports (Dave and Purohit 2002, De Bruyn and Ferreira 1992, Rao and Purohit 2006, Rout et al 2008. Higher cytokinin concentrations (> 2.5 μM) promoted shoot multiplication, and at lower concentrations (< 1.0 μM) shoot elongation was observed.…”

Factors influencing shoot multiplication of lotus (Nelumbo nucifera)

“…Cytokinins are generally considered as a critical factor for in vitro shoot production and there are many reports that BA exhibits beneficial effect over other cytokinins for shoot multiplication (Dantu and Bhojwani 1987, De Bruyn and Ferreira 1992, Rao and Purohit 2006. Previous studies also have shown that for optimal shoot initiation incorporation of both a cytokinin and an auxin in the medium is necessary.…”

“…Nhut 2001, De Bruyn and Ferreira 1992, Guo et al 2005. Although there are a lot of reports on effects of photoperiod and temperature on plant growth in situ, studies for their effects on in vitro plant growth are still very limited (Vaz et al 2004).…”

Mise Au Point Des Techniques De Micropropagation Pour Une Multiplication Massive De Gladiolus Segetum Et Gladiolus Dubius

“…L'absence d'une différence pour la longueur de racines pour les différentes concentrations d'auxines peut être expliquée par un effet prévisible, car ce régulateur de croissance est connu pour ces propriétés de stimulation de la rhizogenèse. Le meilleur taux d'enracinement a été observé sur le milieu MS. Ces résultat n'ont pas en accord avec ceux de (De Bryn et Ferreir 1992), qui ont montré que le milieu MS/2 à donnés le meilleure taux d'enracinement et de bulbaison, ceux-ci peut être expliqué par la forte concentration en glucose et fructose interne chez les bulbes de Gladiolus sp. Cette concentration interne peut donner un effet irréversible.…”