2002
DOI: 10.1179/000349802125002275
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In-vitro cultivation: a sensitive method for detecting <I>Blastocystis hominis</I>

Abstract: Currently, the detection of human infection with Blastocystis hominis is usually based on the examination under a light microscope of faecal samples, either directly, as 'simple smears', or after some form of concentration. Whether short-term, in-vitro cultivation would increase the sensitivity of such detection remains a matter of controversy. Over 900 fresh stool specimens, from soldiers in the Royal Thai Army, were each checked for the parasite using three methods: simple smears; formalin-ethyl-acetate conc… Show more

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Cited by 85 publications
(66 citation statements)
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“…2,5,13 The variations of the prevalence of parasitic infection including Blastocystis infection might depend on the study population or geographic location, age, 14 and detection methods. 15,16 Using PCR technique, the highest prevalence of Blastocystis infection was found among children of Senegal River Basin, which was 100%. 17 In this study, direct wet smear and in vitro cultivation were used as screening detection methods.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…2,5,13 The variations of the prevalence of parasitic infection including Blastocystis infection might depend on the study population or geographic location, age, 14 and detection methods. 15,16 Using PCR technique, the highest prevalence of Blastocystis infection was found among children of Senegal River Basin, which was 100%. 17 In this study, direct wet smear and in vitro cultivation were used as screening detection methods.…”
Section: Discussionmentioning
confidence: 99%
“…growth and sensitivity for Blastocystis detection. 15 Conventional PCR technique was used to amplify DNA only after in vitro cultivation method was positive. Conventional PCR technique did not directly detect Blastocystis sp.…”
Section: Discussionmentioning
confidence: 99%
“…[10][11][12]23 In our study, two media, MBD and TYGM-9, were used for comparison of growth of Blastocystis in culture. These culture media were used instead of Jones medium because it was known that Blastocystis grows successfully in Jones medium.…”
Section: Discussionmentioning
confidence: 99%
“…The use of xenic cultures, in which Blastocystis is grown in vitro with non-specific microorganisms, has been shown to be more sensitive in detecting Blastocystis but it is not commonly used in the diagnostic laboratory. [9][10][11] Molecular diagnosis by polymerase chain reaction (PCR) using the small subunit (SSU) ribosomal RNA gene is becoming more widely used for detection of enteric parasites. Although this technique is more costly, it is known to be more sensitive than the direct smear and xenic culture.…”
Section: Introductionmentioning
confidence: 99%
“…A stool specimen of each enrolled subject was collected and examined for Blastocystis by in vitro cultivation using Jones' medium supplemented with 10% horse serum. 21 Samples were incubated at 37 C for 2-3 days and examined for Blastocystis by light microscopy. Stool specimens of six domestic dogs were also processed by the same method.…”
Section: Methodsmentioning
confidence: 99%