In vitro cultivation and differentiation of fetal liver stem cells from mice

Abstract

During embryonic development, pluripotent endoderm tissue in the developing foregut may adopt pancreatic fate or hepatic fate depending on the activation of key developmental regulators. Transdifferentiation occurs between hepatocytes and pancreatic cells under specific conditions. Hepatocytes and pancreatic cells have the common endodermal progenitor cells. In this study we isolated hepatic stem/progenitor cells from embryonic day (ED) 12-14 Kun-Ming mice with fluorescence-activated cell sorting (FACS). The cells were cultured under specific conditions. The cultured cells deploy dithizone staining and immunocytochemical staining at the 15th, 30th and 40th day after isolation. The results indicated the presence of insulin-producing cells. When the insulin-producing cells were transplanted into alloxaninduced diabetic mice, the nonfasting blood glucose level was reduced. These results suggested that fetal liver stem/ progenitor cells could be converted into insulin-producing cells under specific culture conditions. Fetal liver stem/ progenitor cells could become the potential source of insulin-producing cells for successful cell transplantation therapy strategies of diabetes.

In vitro differentiation of hepatic progenitor cells from mouse embryonic stem cells induced by sodium butyrate

“…Because of these characteristics, ES cells have been an excellent in vitro model system for the studies of key events during early embryo development and may also provide a prospective source of transplantable cells for cell-replacement therapies. Presently remarkable progress has been made in understanding the differentiation of ES cells into neural [Hancock et al, 2000], hematopoietic [Baron, 2001;Perlingeiro et al, 2001;Feng et al, 2005], endothelial , vascular [Sone et al, 2003;Yurugi-Kobayashi et al, 2003;Suzuki et al, 2005], and pancreatic stem/progenitor cells and then various mature cells. However, limited knowledge has been acquired for the differentiation of hepatic progenitor/stem cells, although hepatic differentiation from murine and human ES cells both in vitro and in vivo has been reported in recent studies [Hamazaki et al, 2001;Chinzei et al, 2002;Choi et al, 2002;Ishizaka et al, 2002;Jones et al, 2002;Miyashita et al, 2002;Yamada et al, 2002;Yin et al, 2002;Yamamoto et al, 2003;Kania et al, 2004;Ogawa et al, 2005;Teramoto et al, 2005;Teratani et al, 2005].…”

Mesenchymal progenitor cells combined with pentosan polysulfate mediating disc regeneration at the time of microdiscectomy: a preliminary study in an ovine model

“…Multiple preclinical animal studies have demonstrated the efficacy of intradiscally injected mesenchymal stem cells (MSCs) to induce disc regeneration. 1,2,16,18,20,21,25,[31][32][33][34]38,39,49,53,56,60,[64][65][66]69,73,83,87,90 Allogeneic mesenchymal progenitor cells (MPCs) are the earliest uncommitted clonogenic population of bone marrow stromal cells and have also successfully repaired the extracellular matrix following their injection into degenerative ovine discs. 25 Moreover, when ovine MPCs combined with the chondrogenic agent pentosan polysulfate (PPS) were embedded in a gelatin sponge and placed in a biodegradable cage that was implanted into the interbody space of ovine cervical spines, the cage became filled with a predominately cartilaginous matrix.…”

Differentiation of Stem Cells into Insulin-Producing Cells: Current Status and Challenges

“…Feng et al (2005) indicated that these cells could be converted into insulin-producing cells under specific culture condition (medium supplemented with nicotinamide, L-glutamine, b-mercaptoethanol and glucose). When these cells were transplanted into alloxan-induced diabetic mice, the non-fasting blood glucose level was reduced (Feng et al 2005). …”