In vitro cultivation of cells from the chorioallantoic membrane of chick embryos

Cursiefen, Dagmar; Becht, H.

doi:10.1007/bf02120764

Cited by 14 publications

(9 citation statements)

References 22 publications

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“…Tissue Cultures Primary chick embryo fibroblasts and chorioallantoic membrane (CAM) cells were prepared as described (2,13). The preparation of the CAM tissue cultures was modified as follows: the concentration of EDTA should be 0.02 per cent, and we used 80 mg collagenase and 10 mg hyaluronidase in 500 ml of GKN for the treatment of 30 to 40 CAM membranes from embryonated eggs.…”

Section: Methodsmentioning

confidence: 99%

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Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses

Seto

Wahn

Becht

1980

Archives of Virology

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Chorioallantoic membrane (CAM) tissue cultures were found to be permissive for representative paramyxoviruses. The CAM cells can be used for plaque assay without the presence of trypsin. Ultrastructures of CAM cells infected with paramyxovirus Yucaipa (PMY), Sendai virus, and NDV were different. Nucleocapsids were readily seen in budding structures of cells infected with PMY, and in Sendai virus infected cells, large clusters of nucleocapsids were clearly evident in the cytoplasm. The site of glycoprotein cleavage does not have any effect on the nature of budding. It appears that a factor or factors in addition to the nature of the plasma membrane influences the morphology of cells infected with paramyxoviruses.

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Section: Methodsmentioning

confidence: 99%

“…NAGAI et al (9) reported t h a t chorioallantoic membrane (CAS{) cells (2) in vitro are permissive for apathogenic as well as pathogenic strains of NDV. I n this s t u d y we have found the CAM cells to be permissive for p a r a m y x o v i r u s Yucaipa (PMY) and Sendal, virus.…”

Section: Introductionmentioning

confidence: 99%

Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses

Seto

Wahn

Becht

1980

Archives of Virology

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“…CV-1 cells (TC7 clone) were grown in Eagle's medium with 10% fetal calf serum, and HeLa cells were grown in reinforced Eagle's medium with 5% fetal calf serum as described (1) (Eagle's medium contains 5.5 mM glucose and the reinforced medium contains 22 mM glucose). Primary chicken embryo fibroblasts (CEFs) and chorioallantoic membrane cells were prepared as described (8,9) …”

Section: Abstacmentioning

confidence: 99%

Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose

Peluso

Lamb

Choppin

1978

Proc. Natl. Acad. Sci. U.S.A.

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ABSTACInfection of several types of cultured cells with the paramyxoviruses simian virus 5 and Sendai virus stimulates synthesis of four polypeptides (I-IV) with molecular weights of approximately 99,000,97,000, 86,000, and 78,000, respectively. That these are host polypeptides encoded in cellular mRNAs has been shown by the inhibition of their synthesis by actinomycin D and by the similarity of the peptide maps of them and of polypeptides with the same electrophoretic mobility from uninfected cells. Peptide mapping as well as identical migration in polyacrylamide gels has also indicated that polypeptides I, II, and IV are the same as plasma membrane polypeptides whose synthesis is enhanced in cells transformed by Rous sarcoma virus and in normal cells by glucose deprivation or treatment with 2-deoxyglucose. Polypeptides I and II appear to be the same polypeptides, with the observed differences in migration reflecting the glycosylation of polypeptide I, a relationship-previously shown to exist between polypeptides in glucose-deprived and glucose-fed cells. Infection with paramyxoviruses does not significantly increase the transport of glucose by cells, and the maintenance of a high concentration of glucose in the medium does not prevent the enhanced synthesis of these polypeptides. This is in contrast to the situation in transformedcells in which stimulation of synthesis of these polypeptides is secondary to depletion of glucose in the mediuwr due to increased glucose uptake by the cells. Thus, although paramyxovirus infection and transformation by Rous sarcoma virus result in stimulation of the synthesis of the same membrane polypeptides, the mechanism of stimulation differs.We have reported (1) that, in addition to the viral structural polypeptides and a nonstructural polypeptide, four polypeptides were synthesized in increased amounts in cells infected with the paramyxovirus simian virus 5 (SV5). These polypeptides, designated I-IV, had estimated molecular weights of 99,000, 97,000, 86,000, and 78,000, respectively. It was suggested that these were cellular polypeptides whose synthesis was enhanced by infection because: (i) (4, 7). On the basis of these results, these polypeptides have been referred to as "glucose-regulated proteins" (4). As described here, paramyxovirus infection, unlike transformation, does not cause a significant increase in glucose transport, and maintenance of a high glucose concentration does not prevent the enhanced synthesis of these polypeptides in paramyxovirusinfected cells. Thus, paramyxovirus infection apparently produces a perturbation in the cell membrane, different from transformation, that alters the rate of synthesis of these polypeptides.MATERIALS AND METHODS Cells. CV-1 cells (TC7 clone) were grown in Eagle's medium with 10% fetal calf serum, and HeLa cells were grown in reinforced Eagle's medium with 5% fetal calf serum as described (1) (Eagle's medium contains 5.5 mM glucose and the reinforced medium contains 22 mM glucose). Primary chicken embryo fibroblasts (CEFs) a...

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“…For metabolic labeling NDV was grown in primary chick embryo or in cultured cells of the chorioallantoic membrane (CAM) (Cursiefen and Becht, 1975).…”

Section: Introductionmentioning

confidence: 99%

Acylation of virol. spike glycoproteins: A feature of enveloped RNA viruses

Schmidt

1982

Virology

160

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In vitro cultivation of cells from the chorioallantoic membrane of chick embryos

Cited by 14 publications

References 22 publications

Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses

Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxoviruses

Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose

Acylation of virol. spike glycoproteins: A feature of enveloped RNA viruses

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