In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors2

Giard, Donald J.; Aaronson, Stuart A.; Todaro, George J.; Arnstein, Paul; Kersey, John H.; Dosik, Harvey; Parks, Wade P.

doi:10.1093/jnci/51.5.1417

Cited by 1,999 publications

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“…Subcutaneous tumors were generated in athymic nude mice using the human melanoma cell line A375. 9,10 The initial retroviral transduction efficiency, as judged by the number of G418-resistant colonies rescued from the tumor mass, was similar to that seen in the 9L tumor model ( Figure 2); however, in this model we took the opportunity to follow the extent of retroviral transduction over time. At subsequent time-points (weeks 3 and 4 after injection) a much higher frequency of transduction was observed ( Figure 2); at week 4 up to 7.2% of cells were retrovirally transduced.…”

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confidence: 59%

Adeno-retroviral chimeric viruses as in vivo transducing agents

et al. 1999

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Several hybrid viral gene transfer systems have been all four transcomplementing adeno-retroviruses. Molecular described that exploit the favorable features of the two paranalysis of DNA extracted from 9L G418 R populations ent viral species. We have developed a hybrid adeno-retroderived both in vitro and in vivo showed appropriate inteviral vector system to generate a retroviral vector in situ.gration of the LXSN proviral sequence. Tumor cells were The system consists of adenoviruses encoding MoMLV harvested 1, 3 and 4 weeks after adeno-retrovirus adminisgag.pol (Axtet.gag.pol), the VSV-G viral envelope tration to the human A375 xenografts. The percentage of (Axtet.VSV-G), the retroviral vector LXSN expressing the G418 R colonies recovered from tumors transduced with all neomycin phosphotransferase gene (AV-LXSN) and a of the transcomplementing adeno-retroviruses increased transcriptional regulator to control expression of gag.pol with time, whereas no increase was observed in tumors and envelope (AV-rtTA). In vitro, biologically active retrovitransduced with AV-LXSN alone. DNA extracted from ral vector preparations were generated following adeno-G418 R A375 cell populations showed the presence of interetroviral transduction of 9L rat glioma cells. In vivo the grated proviral sequences only in animals that received the transcomplementing adeno-retroviruses were co-adminisfull complement of adeno-retroviruses. These results demtered intratumorally into subcutaneous 9L glioma tumors in onstrate that adenoviral vectors expressing transcomprats and human A375 melanoma xenografts in nude mice.lementing genes for retroviral proteins and retroviral vector In the 9L rat model, G418 R cell cultures were only obtained RNAs can be used for in situ transduction of target cells. when 9L cells were harvested from tumors injected with Keywords: adenovirus; retrovirus; gene therapy There have been several recent reports describing the use of recombinant viruses of one species to provide transcomplementing functions which support the amplification of replication-deficient variants of a second viral species. 1-8 These reports have primarily been in vitro studies which demonstrate the principle that virusmediated provision of these transcomplementing functions is adequate to support the generation of a second vector. Feng and co-workers 7 although have reported evidence that this can occur in vivo using an adeno-retroviral hybrid system. 7 The system, consisting of two adenoviruses, one carrying a retroviral transcription unit encoding green fluorescent protein (GFP) and a second expressing retroviral gag.pol and env functions, was used to transduce human ovarian tumor cells (SKOV3. p1 ) in vivo. Co-injection of both adenoviruses resulted in more efficient transduction of the tumor compared with administration of the adenovirus carrying the retroviral GFP vector alone. To examine further the potential appli-

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confidence: 59%

Adeno-retroviral chimeric viruses as in vivo transducing agents

et al. 1999

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“…The cell lines MRC-5 [19], WI-38 [20], A549 [21], and Calu-3 [22] were cultured in Dulbecco's minimal essential medium NUT mix F12 (no. 21331-020, Invitrogen, Karlsruhe, Germany), containing 10% FCS (no.…”

Section: Cells and Culture Mediummentioning

confidence: 99%

Tissue-specific induction of ADAMTS2 in monocytes and macrophages by glucocorticoids

et al. 2007

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The regulated expression of ADAMTS2 (a disintegrin and metalloproteinase with thrombospondin motifs), a secreted metalloproteinase involved in the processing of procollagen to collagen, was studied in peripheral blood mononuclear cells (PBMC). Stimulation with glucocorticoids (GC) resulted in a pronounced dose-and timedependent increase of ADAMTS2 mRNA levels in PBMC. The increase of ADAMTS2 expression was specific for CD14++ monocytes (440-fold) and alveolar macrophages (200-fold), whereas CD3+ (T lymphocytes), phytohemagglutinin-activated CD3+ (T lymphocytes), and CD19+ (B lymphocytes) showed no significant changes in ADAMTS2 mRNA after GC treatment. Treatment of monocyte-derived macrophages (MDM) with GC also resulted in an increase of ADAMTS2 protein in the culture tissue media. Using the GC analog RU486, GC-mediated induction of ADAMTS2 mRNA was blocked, implicating that GC acts specifically via the GCreceptor. In agreement with findings in blood monocytes, cell lines of the monocytic lineage (MM6, THP-1) showed significant GC-induced significant increases in ADAMTS2 mRNA, while in epithelial cells (A549, Calu-3, Colo320, BT-20) and fibroblast (MRC-5, WI-38, and two NHDF-c cell types from adult cheek and upper arm), they showed no or little responsiveness to GC. As macrophages have important functions in immune defense and tissue homeostasis, these findings suggest that GC-mediated specific induction of ADAMTS2 in these cells may play a crucial role in the resolution of inflammation and wound repair.

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“…The human breast cancer cell line MCF7 was obtained from American Tissue Culture Collections (ATCC number HTB-22) (Giard et al, 1973;Cailleau et al, 1974). MCF7 cells were cultured at 378C in a 5.5% CO 2 atmosphere in Minimum Essential Medium alpha with ribonucleosides and deoxyribonucleosides, supplemented with 10% fetal calf serum, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids and 0.01 mg/ml gentamycin (Gibco ± BRL, Life Technologies AG, Basel, Switerland).…”

Section: Cell Culturementioning

confidence: 99%