In vitro culture at low temperature and ex vitro acclimatization of Vriesea inflata an ornamental bromeliad

Abstract

− (In vitro culture at low temperature and ex vitro acclimatization of Vriesea inflata an ornamental bromeliad). In vitro culture by seeds is a technique for preservation of threatened species because it may provide a large number of plants with genetic diversity. The bromeliad Vriesea inflata (Wawra) Wawra, an ornamental bromeliad, is extensively and illegally collected from the nature and must be preserved. it is possible to form plant threatened collections in vitro by reducing the temperature of culture, while occupying little space, with the consequent reduction of maintenance costs. This work evaluated the influence of temperature on in vitro growth and morphology of plants of V. inflata, with the aim of establishing a slow growth-rate and analyzing the ex vitro acclimatization. seeds were germinated in vitro in murashige and skoog (ms) medium, with macronutrients reduced to 50% (MS/2). After three months the plants were transferred to flasks of new same medium and kept in two germination chambers with the temperature adjusted to 15 °c and to 28 °c. After 24 months the plants were subject to biometric, photosynthetic pigments content and anatomical analyses. Results showed that plants maintained at 15 °c were smaller than those at 28 °c. nevertheless, there were no alterations in pigments content, anatomy. in both treatments there was a survival rate of 100%. This work showed that plants of this species can be kept in vitro at 15 °c with the aim of forming a slow-growth collection, thereby seeking its preservation, and can be transferred to growth at ex vitro condition to achieved 100% survival rate.key words -anatomy, Bromeliaceae, conservation, growth, photosynthetic pigments RESUMO − (Cultivo in vitro em temperatura baixa e aclimatização ex vitro de Vriesea inflata, uma bromélia ornamental). o cutivo in vitro iniciado a partir de sementes pode ser utilizado para preservação de espécies ameaçadas porque contribui para o fornecimento de um grande número de plantas com diversidade genética. Vriesea inflata (Wawra) Wawra, uma bromélia ornamental, é extensivamente e ilegalmente coletada da natureza, sendo importante sua preservação. Por meio do cultivo in vitro é possível formar coleções de plantas ameaçadas, sob crescimento lento, ocupando pouco espaço, com redução dos custos de manutenção das culturas. Este trabalho avaliou a influência da temperatura sobre o crescimento e morfologia de plantas de V. inflata mantidas in vitro, com objetivo de diminuir o crescimento, além de verificar a aclimatização destas para a condição ex vitro. sementes de V. inflata foram germinadas utilizando-se o meio de murashige and skoog (ms) com macronutrientes reduzidos à metade (MS/2). Após três meses as plantas foram transferidas para frascos contendo o mesmo meio e mantidos em câmaras de germinação, com temperaturas ajustadas para 15 °c e 28 °c. Após 24 meses essas plantas foram submetidas à analises biométricas, anatômicas e de pigmentos fotossintéticos. Verificou-se que as plantas mantidas a 15 °C foram menores qu...

Avaliação da eficiência da propagação de Alcantarea imperialis (Bromeliaceae) cultivada in vitro e ex vitro

“…Após essa etapa, as sementes foram enxaguadas três vezes com água destilada esterilizada. Este procedimento de desinfestação é comumente utilizado para sementes que têm apêndices plumosos como relatado para outras espécies (Aranda-Perez & Martinelli 2009;Pedroso et al 2010).…”

Impacts of photoautotrophic and photomixotrophic conditions on in vitro propagated Billbergia zebrina (Bromeliaceae)

“…Hydrenchyma cells are specialized in storing water. Increased volume of this tissue results in a higher water storage capacity (Pedroso et al 2010;Martins et al 2014). This water-storage parenchyma has a water buffering action, avoiding dehydration of in vitro propagated plantlets during the first days after transplantation, contributing to a higher survival rate (Barboza et al 2006).…”

Conservation of Vriesea flammea L.B.Sm., an endemic Brazilian bromeliad: effects of nutrients and carbon source on plant development

“…Despite the success in germination rate, the use of sterile capsules to obtain plants from seeds germinated in vitro was not very feasible since it led to a high degree of contamination of seeds by fungi and bacteria. The use of both methodologies is quite common for obtaining bromeliad or orchid plants by means of seeds (Droste et al, 2005;Pedroso et al, 2010;Suzuki et al, 2010;Soares et al, 2012;Endres Júnior et al, 2014;Sasamori et al, 2015;. Vriesea gigantea Gaudich., V. philippocoburgii Wawra and V. incurvata Gaudich.…”