In vitro culture for the detection of infectious human parvovirus B19 and B19-specific antibodies using foetal haematopoietic precursor cells

Morey, Adrienne; Patou, Gary; Myint, S.; Fleming, K. A.

doi:10.1099/0022-1317-73-12-3313

Cited by 17 publications

(6 citation statements)

References 21 publications

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“…While the pluripotent stem cell is sheltered, the susceptibility of erythroid progenitors to B19 infection increases with differentiation (332). B19 can be cultured in erythroid progenitor cells from a variety of sources, including human BM (248,249,325), fetal liver (44,204,368), umbilical blood (322,326), and peripheral blood (297,302). In all culture systems erythropoietin is required to maintain viral replication, probably by supporting the rapid division of erythroid progenitors.…”

Section: Culturementioning

confidence: 99%

Human Parvovirus B19

2002

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Parvovirus B19 (B19) was discovered in 1974 and is the only member of the family Parvoviridae known to be pathogenic in humans. Despite the inability to propagate the virus in cell cultures, much has been learned about the pathophysiology of this virus, including the identification of the cellular receptor (P antigen), and the control of the virus by the immune system. B19 is widespread, and manifestations of infection vary with the immunologic and hematologic status of the host. In healthy immunocompetent individuals B19 is the cause of erythema infectiosum and, particularly in adults, acute symmetric polyarthropathy. Due to the tropism of B19 to erythroid progenitor cells, infection in individuals with an underlying hemolytic disorder causes transient aplastic crisis. In the immunocompromised host persistent B19 infection is manifested as pure red cell aplasia and chronic anemia. Likewise, the immature immune response of the fetus may render it susceptible to infection, leading to fetal death in utero, hydrops fetalis, or development of congenital anemia. B19 has also been suggested as the causative agent in a variety of clinical syndromes, but given the common nature, causality is often difficult to infer. Diagnosis is primarily based on detection of specific antibodies by enzyme-linked immunosorbent assay or detection of viral DNA by dot blot hybridization or PCR. Treatment of persistent infection with immunoglobulin reduces the viral load and results in a marked resolution of anemia. Vaccine phase I trials show promising results

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Section: Culturementioning

confidence: 99%

Human Parvovirus B19

2002

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“…Enrichment of erythroid progenitor cells from bone marrow can support a higher viral replication [45]. Erythroid progenitor cells susceptible to B19V infection can be also obtained from peripheral blood [46,47], from fetal liver [48][49][50], and from umbilical cord blood [51,52]. More recently, advances in techniques for generating large numbers of human erythroid progenitor cells (EPCs) ex vivo from circulating peripheral blood hematopoietic stem cells (HSCs) have been exploited [53,54].…”

Section: Isrn Virologymentioning

confidence: 99%

Parvovirus B19 Achievements and Challenges

Gallinella

2013

ISRN Virology

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Parvovirus B19 is a widespread human pathogenic virus, member of the Erythrovirus genus in the Parvoviridae family. Infection can be associated with an ample range of pathologies and clinical manifestations, whose characteristics and outcomes depend on the interplay between the pathogenetic potential of the virus, its adaptation to different cellular environments, and the physiological and immune status of the infected individuals. The scope of this review is the advances in knowledge on the biological characteristics of the virus and of virus-host relationships; in particular, the interactions of the virus with different cellular environments in terms of tropism and ability to achieve a productive replicative cycle, or, on the contrary, to establish persistence; the consequences of infection in terms of interference with the cell physiology; the process of recognition of the virus by the innate or adaptive immune system, hence the role of the immune system in controlling the infection or in the development of clinical manifestations. Linked to these issues is the continuous effort to develop better diagnostic algorithms and methods and the need for development of prophylactic and therapeutic options for B19V infections.

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“…Globoside is also found in endothelial cells, which may be the site of B19 replication resulting in fifth disease (14). The presence of globoside in fetal cardiac myocytes may lead to B19 infection and myocarditis (15,16).…”

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confidence: 99%

Cryo-electron microscopy studies of empty capsids of human parvovirus B19 complexed with its cellular receptor.

Chipman

Agbandje‐McKenna

Brown

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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The three-dimensional structures of human parvovirus B19 VP2 capsids, alone and complexed with its cellular receptor, globoside, have been determined to 26 A resolution. The B19 capsid structure, reconstructed from cryo-electron micrographs of vitrified specimens, has depressions on the icosahedral 2-fold and 3-fold axes, as well as a canyon-like region around the 5-fold axes. Similar results had previously been found in an 8 A resolution map derived from x-ray diffraction data. Other parvoviral structures have a cylindrical channel along the 5-fold icosahedral axes, whereas density covers the 5-fold axes in B19. The glycolipid receptor molecules bind into the depressions on the 3-fold axes of the B19:globoside complex. A model of the tetrasaccharide component ofgloboside, organized as a trimeric fiber, fits well into the difference density representing the globoside receptor. Escape mutations to neutralizing antibodies map onto the capsid surface at regions immediately surrounding the globoside attachment sites. The proximity of the antigenic epitopes to the receptor site suggests that neutralization of virus infectivity is caused by preventing attachment of viruses to cells.Parvovirus B19 is the only member of Parvoviridae pathogenic to man (1, 2). Acute infection causes a childhood measles-like rash condition, erythrema infectosium, also known as fifth disease, and acute or chronic arthritis in adults. In persons with underlying hemolysis, acute B19 infection results in transient aplastic crises, due to cessation in red blood cell production, which is caused by tropism of B19 to erythroid progenitor cells. In immunocompromised individuals, such as AIDS patients and patients undergoing immunosuppressive drug therapy, persistent B19 infection causes chronic severe anemia due to erythroid marrow failure. B19 infection of pregnant women may cause hydrops fetalis (congestive heart failure) of the fetus and spontaneous abortions due to the inability of the fetus to mount an adequate immune response.Parvoviruses have a single-stranded DNA genome encapsidated in a T = 1 (60 structurally equivalent subunits) icosahedral protein capsid, approximately 260 A in diameter.The major structural protein, VP2, has a molecular mass of 58,000 and consists of an eight-stranded, anti-parallel, (3-barrel motif (with , strands B, C, . . . , I), similar to that found in many other viral capsid proteins (3). This (3-barrel structure contains only about one-third of the amino acid content in each polypeptide and lies mostly below the capsid surface, which is formed by large insertions between the /3-strands. For canine parvovirus (CPV) (4) and feline panleukopenia virus (FPV) (5), the loops between the B and C (loop 1), E and F (loop 2), and G and H (loops 3 and 4) (3-strands contain 36, 74, and 223 residues, respectively. These loops form most of the capsid surface, with loops 3 and 4 forming the bulk of the spikes that extend 22 A radially outwards around the 3-fold axes. The insertion of 20 amino acids between the D and E st...

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In vitro culture for the detection of infectious human parvovirus B19 and B19-specific antibodies using foetal haematopoietic precursor cells

Cited by 17 publications

References 21 publications

Human Parvovirus B19

Human Parvovirus B19

Parvovirus B19 Achievements and Challenges

Cryo-electron microscopy studies of empty capsids of human parvovirus B19 complexed with its cellular receptor.

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