In Vitro Culture of Bovine Embryos With a Serum Replacer Improves Survival Following Vitrification and Warming

Johnson, Sarah E.; Wroclawska, Ewa; Bonilla, A. Q.; Moore, Karen J.

doi:10.1093/biolreprod/77.s1.132

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“…Survival can be defined as re-expansion and continued development with normal morphology in culture following vitrification and warming (Johnson et al ., 2007). When the re-expansion rate was assessed, there was no difference between IVP groups in the presence or absence of FCS.…”

Section: Discussionmentioning

confidence: 99%

“…The lack of a positive effect of serum supplementation may be a consequence of the differences in experimental conditions, such as the absence of a co-culture system during all developmental periods and the type and concentration of serum used (Mucci et al ., 2006). Therefore, in another experiment, the presence of FCS led to a higher re-expansion rate of embryos (Johnson et al ., 2007).…”

Section: Discussionmentioning

confidence: 99%

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Forskolin effect on the cryosurvival ofin vitro-produced bovine embryos in the presence or absence of fetal calf serum

Paschoal

Sudano

Guastali

et al. 2012

Zygote

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The objective of this study was to assess the viability and cryotolerance of zebu embryos produced in vitro with or without the addition of fetal calf serum (FCS) and forskolin (F). Embryos produced in vivo were used as a control. Presumptive zygotes were cultured in modified synthetic oviductal fluid supplemented with amino acids (SOFaa), bovine serum albumin (BSA) and with (2.5%) or without (0%) FCS. On day 6 of growth, the embryos from each group were divided into treatments with or without 10 μM F to induce embryonic lipolysis, comprising a total of four experimental groups: 2.5% FCS, 0% FCS, 2.5% + F and 0% + F. For vitrification, embryos were exposed to vitrification solution 1 (5 M EG (ethylene glycol)) for 3 min and then transferred to vitrification solution 2 (7 M EG, 0.5 M galactose solution and 18% (w/v) Ficoll 70) before being introduced to liquid nitrogen. The presence of FCS in the culture medium resulted in the production of embryos with a similar rate of damaged cells compared with in vivo-produced embryos. After vitrification, the 2.5% FCS group had a significantly higher rate of damaged cells when compared with the other groups (P < 0.05). The results of this experiment indicated that the omission of FCS and the addition of forskolin do not have deleterious effect on embryo production rates. In addition, embryos produced in the presence of FCS had greater sensitivity to cryopreservation, but this effect was reversed when forskolin was added to the medium, which improved embryo survival without affecting embryo development and quality after vitrification.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%