In vitro culture of hFOB1.19 osteoblast cells on TGF-β1-SF-CS three-dimensional scaffolds

Tong, S. Y.; Xue, Lei; Xu, Di; Liu, Zi-mei; Du, Youwei; Wang, Xukai

doi:10.3892/mmr.2015.4498

Cited by 11 publications

(7 citation statements)

References 40 publications

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“…It was necessary to estimate the toxicity of the obtained SeB-NPs and Ag SeB-NPs for further tissue engineering applications. Osteoblasts were commonly used to evaluate the cytocompatibility of nanoparticles and/or scaffold in tissue engineering 58,59 . In this study, the cytocompatibility of SeB-NPs and Ag SeB-NPs was assessed by culturing with hFOB 1.19 cells.…”

Section: Discussionmentioning

confidence: 99%

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Nie

Hou

Wang

et al. 2020

Sci Rep

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Selenium-doped nanostructure has been considered as an attractive approach to enhance the antibacterial activity of calcium phosphate (cap) materials in diverse medical applications. in this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, silver was in situ incorporated into SeB-NPs to obtain nanostructured composite nanoparticles (Ag SeB-NPs). Both SeB-NPs and Ag SeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO 3 2− was doped at the po 4 3− position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared Ag SeB-NPs had a needlecluster-like morphology. CCK-8 analysis revealed SeB-NPs and Ag SeB-NPs had good cytocompatibility with osteoblasts. the antibacterial activity of the prepared Ag SeB-NPs was confirmed by using Gramnegative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final Ag SeB-NPs for biomedical applications. Tissue engineering has been widely used in bone graft and dentistry due to a large number of patients require grafting resulting from congenital conditions, trauma, and tumor resection, and so on 1-3. Mineralized tissues, such as bone, tooth enamel, dentin, and cementum, are enriched with significant amounts of ionic substitutions, including sodium, potassium, and carbonate groups. These elements can affect the functionality of related tissues 4,5. Calcium phosphate (CaP) based nanoparticles, including hydroxyapatite (Ca 10 (PO 4) 6 (OH) 2 , HA), β-tricalcium phosphate (Ca 3 (PO 4) 2 , β-TCP), tetracalcium phosphate (Ca 4 (PO 4) 2 O; TTCP), biphasic calcium phosphate (BCP), and so forth, were well-known in implant surgery because of their excellent biocompatibility, biodegradability, and tunable physicochemical properties. Compared to HA or β-TCP, biphasic calcium phosphate (BCP) composed of HA and β-TCP, performed better mechanical properties, higher biological activity, and adjustable degradation rate 6-10. Bacteria attached to the implanted medical devices or scaffold can cause healthcare-associated infections, such as the formation of biofilm, which further results in local inflammation and infection. Therefore, the scaffold with excellent antibacterial properties provides an overwhelming advantage in the clinic stage 11,12. Many metal nanoparticles, such as silver (Ag), zinc oxide (ZnO), and titanium dioxide (TiO 2), performed strong antibacterial properties and low toxicity towards mammalian cells, some of these nanoparticles have been widely applied in a range of areas 13-20. Silver (Ag) is stable in the body fluids and can be used in antibacterial implants due to silver can easily binds to bacterial DNA and RNA, resulting in bacteria death 21. However, the antibacterial effect of nanoparticles was influenced...

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Section: Discussionmentioning

confidence: 99%

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Nie

Hou

Wang

et al. 2020

Sci Rep

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“…On day 21 after the start of osteogenic induction, the cells were fixed with 4% paraformaldehyde. Calcium nodules were stained with 0.2% alizarin red and observed using a microscope(CKX41, Olympus, Tokyo, Japan) 26) .…”

Section: Preparation and Identifi Cation Of Bmscsmentioning

confidence: 99%

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Yue

Tan

Zhao

et al. 2018

J. Hard Tissue Biology.

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In bone tissue engineering, scaffold fabrication and biocompatibility are crucial concerns. Many scaffold materials have been explored, among which nanometer hydroxyapatite (nHAP) and collagen (COL) are commonly used. Additionally, growth factors can be used to modify scaff olds. In this study, lyophilization technology was used to build a scaff old comprising basic fi broblast growth factor (bFGF), nHAP and COL for the fi rst time. The resulting scaff old was characterized. bFGF release from the scaff old was assessed by ELISA. Bone marrow mesenchymal stem cells (BMSCs) were prepared and seeded onto the scaff old to test in vitro biological compatibility. A scanning electron microscope was used to observe the scaff old and evaluate BMSC morphology, and the cells were counted to detect early cell adhesion. Cell proliferation and activity were assessed by a cell counting kit-8 assay and measurement of alkaline phosphatase activity, respectively. Bilateral mandibular defects were prepared in 12 New Zealand rabbits and repaired using scaff olds. The rabbits were divided into four groups: a group treated with allogeneic BMSC-seeded bFGF/nHAP/COL scaff old, a group treated with allogeneic BMSC-seeded nHAP/COL scaff old, a group treated with nHAP/COL scaff old alone, and an untreated control group. After 12 weeks, three-dimensional computerized tomography examination, computerized tomography value measurement, gross observation and hematoxylin and eosin stain staining were conducted. SPSS17.0 software was used for data analysis. The gross morphology conformed to the characteristics of a tissue engineering scaff old. The bFGF/nHAP/COL scaff old promoted BMSC adhesion, proliferation and diff erentiation and hence promoted good bone formation, without exhibiting biological toxicity. Our fi ndings show that the bFGF/nHAP/COL scaff old has good physical properties and biocompatibility in vitro, and can be used to promote osteogenesis after in vivo implantation.

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“…In vitro, it has been proved that HAOBs have stronger osteogenic capability than marrow stromal stem cells [15]. In the current study, HOABs were successfully cultured as previously reported [16, 17] and functions of BBR in HAOB proliferation and osteogenic differentiation were investigated.…”

Section: Discussionmentioning

confidence: 69%

Berberine Promotes the Proliferation and Osteogenic Differentiation of Alveolar Osteoblasts through Regulating the Expression of miR-214

Liu¹,

Xu²

2020

Pharmacology

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Introduction and Objective: Alveolar osteoblasts have critical functions during alveolar bone regeneration. Berberine (BBR) and microRNAs (miRNAs) are considered to play important roles in regulating osteoblast differentiation. The study aimed to investigate the role and mechanisms of BBR in osteogenic differentiation of human alveolar osteoblasts (HAOBs) and determine miR-214 expression in the process. Methods: Healthy human alveolar bones were cultured in vitro and prepared for morphological observation and alkaline phosphatase (ALP) staining. The third generation of HAOBs was used for cell transfection and treated by different concentrations of BBR. Cell Counting Kit-8 was used to detect the effect of BBR and increased miR-214 on the proliferation of HAOBs. qRT-PCR and Western blot were used to detect the expression of osteogenic differentiation-related genes and miR-214 level, respectively. Results: The ALP staining results were positive, indicating that cultured cells were HAOBs. Different concentrations of BBR significantly promoted the proliferation of HAOBs and increased the expression levels of ALP, osteocalcin (OCN), collagen type I alpha 1 (COL1A1), runt related transcription factor 2 (RUNX2), and osterix (OSX). Moreover, the expression of miR-214 was reduced as BBR concentrations increased, and the increase of miR-214 reversed the BBR-induced proliferation and osteogenic differentiation of HAOBs. Conclusion: BBR could promote the proliferation and osteogenic differentiation of HAOBs through downregulating the expression of miR-214.

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In vitro culture of hFOB1.19 osteoblast cells on TGF-β1-SF-CS three-dimensional scaffolds

Cited by 11 publications

References 40 publications

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Berberine Promotes the Proliferation and Osteogenic Differentiation of Alveolar Osteoblasts through Regulating the Expression of miR-214

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