In vitro culture of Hibiscus rosa-sinensis L.: Influence of iron, calcium and BAP on establishment and multiplication

Abstract

Some factors influencing in vitro cultures of potted Hibiscus rosa-sinensis L. using nodal cuttings were investigated. A protocol using a modified MS medium helped to overcome chlorosis, shoot tip necrosis (STN) and leaf drop. These disorders have been caused by mineral imbalance associated with calcium and iron deficiency. STN and leaf drop were overcome by increasing calcium level from 3 mM (MS standard concentration) to 9 mM, and this increase, in addition, enhanced shoot dry weight and shoot extension. The chlorophyll content and leaf area increased by increasing the iron concentration 3-fold from 98 lM to 295 lM. Furthermore, substituting Fe-EDTA with Fe-EDDHA resulted in an increase in chlorophyll content, leaf area and shoot extension. The most suitable multiplication medium for H. rosa-sinensis L. was demonstrated to be a modified MS medium containing 2.2 lM BAP and increased concentrations of calcium at 9 mM and iron at 295 lM provided as Fe-EDDHA. The shoots were rooted in half-strength modified MS medium containing 2.7 lM NAA. Acclimatization was successful with all shoots with or without roots.

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

“…In this case, plant tissue culture techniques may be able to facilitate the large-scale production of valuable clones to avoid further depletion of this species from its natural habitat (Conde et al, 2008). Significant features of in vitro propagation are the enormous multiplication capacity in a relatively short time span, the production of healthy and disease-free plants, and the ability to generate propagules throughout the year independent of seasonal changes (Christensen and Sriskandarajah, 2008). Additionally, it is also a useful method to propagate endangered plant species with the purpose of optimizing the time and cost required to aid in preservation (Christensen and Sriskandarajah, 2008).…”

“…Significant features of in vitro propagation are the enormous multiplication capacity in a relatively short time span, the production of healthy and disease-free plants, and the ability to generate propagules throughout the year independent of seasonal changes (Christensen and Sriskandarajah, 2008). Additionally, it is also a useful method to propagate endangered plant species with the purpose of optimizing the time and cost required to aid in preservation (Christensen and Sriskandarajah, 2008). In view of the importance and advantages of plant tissue culture, this study aimed to determine the effects of supplementation of various plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila.…”

Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants

“…The decrease of leaf chlorophyll content in in vitro grown plants can limit the photosynthetic rate by reducing light absorption (CHRISTENSEN et al, 2008). In this study, for fresh weight, chlorophyll a, chlorophyll b, and carotenoids, the flasks with two membranes were superior to the others; although the treatment with one membrane was more efficient in increasing these characteristics than the treatment without membrane (Table 1).…”

Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration

“…Due to the strikingly encouraging effects of plant growth regulator (PGR) in accelerating, inducing, and maintaining in vitro shoots, direct in vitro shoots establishment and multiplication from explants often involve the application of PGRs such as 6-benzylaminopurine (BAP) [12,13], zeatin [14], BAP plus kinetin (KIN) [15], BAP plus indole-3-acetic acid (IAA) [16], or BAP plus naphthalene-3-acetic acid (NAA) [17]. Christensen et al [13]) reported that the most suitable multiplication medium for H. rosa-sinensis L. was demonstrated to be a modified Murashige and Skoog (MS) medium containing 2.2 μM BAP and increased concentrations of calcium at 9 mM and iron at 295 μM provided as ethylenediamine di-2-hydroxyphenyl acetate ferric (Fe-EDDHA).…”

“…Christensen et al [13]) reported that the most suitable multiplication medium for H. rosa-sinensis L. was demonstrated to be a modified Murashige and Skoog (MS) medium containing 2.2 μM BAP and increased concentrations of calcium at 9 mM and iron at 295 μM provided as ethylenediamine di-2-hydroxyphenyl acetate ferric (Fe-EDDHA). Bhalla et al [18] modified the MS medium strength coupled with BAP in inducing direct shooting from H. rosa-sinensis but concluded that BAP was not suitable in their study.…”