In vitro culture of Hypericum rumeliacum Boiss. and production of phenolics and flavonoids

Abstract

The Balkan endemic species, Hypericum rumeliacum, Guttiferae was introduced in vitro for the first time with the aim to study the type of morphogenetic response to plant growth regulators and ability to produce phenolics and flavonoid compounds. The morphoregulatory effect of 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphtaleneacetic acid (NAA), 6-benzyladenine (BA) and combination of BA with NAA in Murashige-Skoog's basal medium on leaf lamina, internode stem segment, stem node and root cuttings was studied. Histological analysis of the structures regenerated from the primary explants proved the presence of both, embryoids and meristemoids. The node explants cultivated on BA-supplemented medium were the most favourable for regeneration through meristemoids. Therefore a double-stage culture approach, allowing an effective multiplication of large quantities of plant shoots in vitro along with maintenance of the biosynthetic capacity of the culture was developed. It comprised one subculture of three-nodal stem explants derived from the stock shoot cultures on MS medium supplemented with 0.2 mg/l BA followed by subculture of the induced multiple shoots on cytokinin-free MS medium. Determination of the total phenolics and flavonoids showed that the decrease of the levels of these secondary metabolites is transitional, as the exclusionof BA from the medium resulted in an increase of their total content.

Agrobacterium enhances xanthone production in Hypericum perforatum cell suspensions

“…Concerning our previous results for phenylpropanoid production in H. perforatum cell cultures, jasmonic acid and salicylic acid were considered as effective elicitors resulting in an increase (up to sixfold) of TP, TF and TFL contents (Gadzovska et al 2007. However, it has been recently proposed that the composition of the culture medium can modify the levels of endogenous phenolics of Hypericum explants (Danova et al 2010). For every particular experiment (effects of A. tumefaciens and A. rhizogenes on cell suspension cultures) a single medium was used.…”

Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum

“…Similarly, work with other Hypericum spp. shoot cultures showed decrease in TP and TF levels upon media supplementation with BA (Danova et al 2010) and MS vitamins (Danova et al 2012). Taken together, these findings indicate that H. perforatum shoot cultures did not give clear cut answer to exogenously applied elicitors or other media nutrients, but cells showed remarkably fast and strong response in the production of phenolic compounds.…”

Hypericum sp.: essential oil composition and biological activities

“…This technology mostly offers the possibility of having a controlled system of production, ensuring a continuous supply of products with uniform quality and yield (Lila 2005). Successful protocols of in vitro micropropagation of many Hypericum species have already been reported (Cardoso and De Oliveira 1996;Moura 1998;Baruah et al 2001;Guedes et al 2003;Franklin and Dias 2006;Bernardi et al 2007;Guedes 2009;Danova et al 2010). However, H. perforatum is the species more intensively studied in terms of shoot regeneration from leaves (Pretto and Santarém 2000;Wójcik and Podstolski 2007), hypocotyls (Murch et al 2000), shoot cuttings (Cellarova et al 1995), stamens (Kirakosyan et al 2000) and roots (Zobayed and Saxena 2003;Cui et al 2010).…”