In vitro culture of immature tassels of an inbred field variety of Zea mays, cv. Oh43

Pareddy, D. R.; Greyson, R. I.

doi:10.1007/bf00040308

Cited by 28 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vasil [26] documented the importance of Kn for the completion of meiosis in cultured anthers of Allium cepa. Kn is also required for the maturation of anthers in Zea tassel inflorescence culture [16,17,18,19] and the present observations are in agreement with these reports. However, in contrast to maize tassel culture, the young ears (Size Class A) cultured in Kn medium promoted the development of normal male spikelets in the place of female spikelets.…”

Section: Maturation O F Ovary and Silksupporting

confidence: 93%

Maturation of stamens and ovaries on cultured ear inflorescences of maize (Zea mays L.)

Bommineni

1990

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Observations were made on the maturation of stamens and ovaries from cultured maize (Zea mays L.) ear inflorescences. Immature ears (5.1-10.0mm long) of maize were cultured in kinetin medium to study microsporogenesis and pollen maturation in developing stamens. Male spikelets developed on ears cultured in kinetin medium. Meiosis-I began by 7 days of culture in the developing anthers and the mature tri-nucleate pollen grains were developed by 20 days of culture. Further, kinetin was required in the culture medium for at least initial 5 days to obtain the microspores in differentiated stamens.To observe the embryosac formation in developed ovaries, ears were cultured in control, kinetin (10.1-15.0 mm long ears) medium, and kinetin+gibberellic acid (5.1-10.0 mm long ears) medium. Formation of embryosacs was noticed in the developed ovaries which were sampled after 20 days of culture. This differential flower development using two growth regulators provides an opportunity to uncover the biochemistry and physiology of micro-and mega-gametophyte development in maize.

show abstract

Section: Maturation O F Ovary and Silksupporting

confidence: 93%

Maturation of stamens and ovaries on cultured ear inflorescences of maize (Zea mays L.)

Bommineni

1990

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…In 1985, Polowick & Greyson showed that the cytological processes in culture leading to mature pollen closely matched those in vivo . Pareddy & Greyson (1985) added further refinements for successful tassel culture leading to germinable pollen grains by optimising concentrations of sucrose (0 .3 M) and kinetin (10 -7 M) together with the addition of casein hydrolysate (30 mg 1 -1 ) . Pareddy et al .…”

Section: The Tasselmentioning

confidence: 99%

Grass inflorescence and spikelet culture: An appraisal

Chapman

1995

Euphytica

View full text Add to dashboard Cite

Techniques of inflorescence and spikelet culture have been used for three major cereals . In maize, the influence of hormones on sexuality has been demonstrated . For wheat and barley the influence of supplying carbohydrate and nitrogen on grain content has been explored . Although in vitro seed setting is less efficient than in vivo the resulting grains, from optimal treatments, are in almost all respects normal or nearly so . Culture media to achieve this are precisely defined .In reviewing this work a major concern has been to highlight an elegant system of enquiry but at the same time to indicate the remarkably small area of interest to which it has been applied . As a prelude therefore to a consideration of the technical achievements, the range of spikelet form, and more particularly, function are examined . The aim is to encourage the application of inflorescence and spikelet culture to a much wider range of topics than hitherto .

show abstract

“…Approaches have been developed to study pollen formation under controlled conditions, such as the in vitro culture of maize tassels, a technique that has been used successfully in severa1 genotypes (Parredy and Greyson, 1985;Parredy et al, 1989). Another approach, which, so far, has had limited success, involves maturation of pollen in vitro from cultured microspores (Dupuis and Pace, 1992).…”

Section: Pollen Quality: a Prerequisite To Gamete Lsolationmentioning

confidence: 99%

Gametes and Fertilization: Maize as a Model System for Experimental Embryogenesis in Flowering Plants

Dumas¹,

Mogensen²

1993

The Plant Cell

View full text Add to dashboard Cite

In vitro culture of immature tassels of an inbred field variety of Zea mays, cv. Oh43

Cited by 28 publications

References 20 publications

Maturation of stamens and ovaries on cultured ear inflorescences of maize (Zea mays L.)

Maturation of stamens and ovaries on cultured ear inflorescences of maize (Zea mays L.)

Grass inflorescence and spikelet culture: An appraisal

Gametes and Fertilization: Maize as a Model System for Experimental Embryogenesis in Flowering Plants

Contact Info

Product

Resources

About