In vitro culture of reptile PGCS to preserve endangered species

Golkar‐Narenji, Afsaneh; Dzięgiel, Piotr; Kempisty, Bartosz; Petitte, James N.; Mozdziak, Paul; Bryja, Artur

doi:10.1002/cbin.12033

Cited by 4 publications

(1 citation statement)

References 117 publications

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“…Several molecular mechanisms and signaling pathways are active during mammalian PGC specification [7,8]. In mice, BMP4 induces BLIMP1 and PRDM14 expression, along with AP2γ and TFAP2C, leading to PGCs fate.…”

Section: Introductionmentioning

confidence: 99%

Porcine Germ Cells Phenotype during Embryonic and Adult Development

Jorge

Recchia

Glória

et al. 2023

Animals

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Primordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in domestic animals, e.g., swine, regarding the epigenetic and pluripotency profiles of PGCs during development. This study analyzed the expression of epigenetic and various pluripotent and germline markers associated with the development and differentiation of PGCs in porcine (pPGCs), aiming to understand the different gene expression profiles between the genders. The analysis of gonads at different gestational periods (from 24 to 35 days post fertilization (dpf) and in adults) was evaluated by immunofluorescence and RT-qPCR and showed phenotypic differences between the gonads of male and female embryos. In addition, the pPGCs were positive for OCT4 and VASA; some cells were H3k27me3 positive in male embryos and adult testes. In adults, the cells of the testes were positive for germline markers, as confirmed by gene expression analysis. The results may contribute to understanding the pPGC pathways during reproductive development, while also contributing to the knowledge needed to generate mature gametes in vitro.

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Section: Introductionmentioning

confidence: 99%

Porcine Germ Cells Phenotype during Embryonic and Adult Development

Jorge

Recchia

Glória

et al. 2023

Animals

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The universal labeling and sorting of avian primordial germ cell utilizingLycopersicon Esculentumlectin

Iikawa,

Morita,

Nishina

et al. 2024

Preprint

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Avian species serve as vital resources in human society, with the preservation and utilization of these species heavily reliant on primordial germ cells (PGCs). However, methods for effectively isolating these rare cells alive from embryos remain elusive in avian species beyond chickens. Even within chicken species, existing techniques have shown limited efficiency. In our study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, utilizing Lycopersicon Esculentum lectin. Significantly, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative potential of chicken PGCs. This approach is anticipated to make a significant contribution to the conservation, research, and agricultural industries related to birds worldwide.

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Primary Cell Culture as a Model System for Evolutionary Molecular Physiology

Harper

2024

IJMS

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Primary cell culture is a powerful model system to address fundamental questions about organismal physiology at the cellular level, especially for species that are difficult, or impossible, to study under natural or semi-natural conditions. Due to their ease of use, primary fibroblast cultures are the dominant model system, but studies using both somatic and germ cells are also common. Using these models, genome evolution and phylogenetic relationships, the molecular and biochemical basis of differential longevities among species, and the physiological consequences of life history evolution have been studied in depth. With the advent of new technologies such as gene editing and the generation of induced pluripotent stem cells (iPSC), the field of molecular evolutionary physiology will continue to expand using both descriptive and experimental approaches.

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In vitro culture of reptile PGCS to preserve endangered species

Cited by 4 publications

References 117 publications

Porcine Germ Cells Phenotype during Embryonic and Adult Development

Porcine Germ Cells Phenotype during Embryonic and Adult Development

The universal labeling and sorting of avian primordial germ cell utilizingLycopersicon Esculentumlectin

Primary Cell Culture as a Model System for Evolutionary Molecular Physiology

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