In Vitro Culture of the Olive: An Overview of the Present Scientific Status

Rugini, E.

doi:10.17660/actahortic.1990.286.16

Cited by 16 publications

(25 citation statements)

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“…However, olive is characterized by strong in vitro apical dominance (Rugini and Panelli 1993a), with little formation of secondary axillary shoots, thus limiting the in vitro micropropagation potential. Zeatin, the most utilized cytokinin for shoot proliferation of olive (Rugini 1990;Grigoriadou et al 2002), is required in high concentrations, and is relatively expensive (Rugini and Baldoni 2004). Synthetic growth regulators such as BAP, TDZ and Kinetin, induce the formation of short shoots and big basal callus in olive (Rugini 1990).…”

Section: Ibamentioning

confidence: 99%

Stimulation of node and lateral shoot formation in micropropagation of olive (Olea europaea L.) by using dikegulac

Gyves

Mira²,

Ruiu

et al. 2007

Plant Cell Tiss Organ Cult

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Dikegulac, a growth regulator, was shown to stimulate in vitro shoot multiplication of olive cultivars Canino, Frantoio, Moraiolo, but not cultivars Rosciola and Piantone di Moiano. The Rugini Olive Medium including zeatin (4.5 lM) was used supplemented with a range of dikegulac concentrations (0-133.4 lM). An optimal result in number of shoots and nodes was obtained on cultivars Canino, Frantoio and Moraiolo at 66.7 lM dikegulac. Higher concentrations did not stimulate additional shoot and node formation and resulted in a drastic reduction in height of shoots. Elongated shoots were rooted and acclimatised and showed normal development compared to control plants.

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Section: Ibamentioning

confidence: 99%

Stimulation of node and lateral shoot formation in micropropagation of olive (Olea europaea L.) by using dikegulac

Gyves

Mira²,

Ruiu

et al. 2007

Plant Cell Tiss Organ Cult

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“…Seuls des essais de bouturage herbacé de la variété Chemlal ont déjà été réalisés, mais avec un taux d'enracinement faible (19 %). En revanche, des essais d'utilisation de techniques de culture in vitro sur d'autres variétés d'olivier ont montré des taux d'enracinement supérieurs à 80 % (Rugini, 1990). Après avoir déterminé la période de l'année la plus propice au prélèvement de boutures, un protocole d'enracinement des vitroplants a été établi.…”

Section: L'unclassified

Production de vitroplants d’Olea europea var. Chemlal

Yakoub-Bougdal¹,

Chérifi²,

Bonaly³

2007

Cahiers Agricultures

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“…Unfortunately, tissues of field-grown plants are highly contaminated. Consequently, it is difficult to obtain sterile explants suitables for in vitro tissue culture protocols (Rugini 1990 ).…”

Section: Introductionmentioning

confidence: 99%

Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds

et al. 2016

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Establishment of an efficient explants surface disinfection protocol is essential for in vitro cell and tissue culture as well as germplasm conservation, such as the case of Grapevine (Vitis spp.) culture. In this research, different procedures for disinfection and regeneration of field-grown grapevine cv. ‘Flame seedless’ axillary buds were evaluated. The buds were disinfected using either NaOCl or allyl, benzyl, phenyl and 2-phenylethyl isothiocyanates. Two different media for shooting and four media for rooting were tested. Shoot and root development per buds were registered. The best disinfection procedure with 90 % of tissue survival involved shaking for 60 min in a solution containing 20 % Clorox with 50 drops/L Triton® X-100. These tissues showed the potential to regenerate a complete plant. Plant regeneration was conducted using full strength Murashigue and Skoog (MS) medium supplemented with 8 µM benzyl aminopurine for shoot induction and multiplication, whereas rooting was obtained on half strength MS supplemented with 2 mg L−1 of indole-3-butyric acid and 200 mg L−1 of activated charcoal. In this work, it was designed the protocols for obtaining sterile field-grown grapevine buds and in vitro plant development. This methodology showed potential to produce vigorous and healthy plants in 5 weeks for clonal grapevine propagation. Regenerated plants were successfully established in soil.

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In Vitro Culture of the Olive: An Overview of the Present Scientific Status

Cited by 16 publications

References 0 publications

Stimulation of node and lateral shoot formation in micropropagation of olive (Olea europaea L.) by using dikegulac

Stimulation of node and lateral shoot formation in micropropagation of olive (Olea europaea L.) by using dikegulac

Production de vitroplants d’Olea europea var. Chemlal

Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds

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