In vitro cultures of Cinchona species

Hay, Christoper; Anderson, L. A.; Roberts, Margaret; Phillipson, J.

doi:10.1007/bf00269704

Cited by 29 publications

(4 citation statements)

References 13 publications

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“…The effect of sucrose differs from the findings of Harkes et a!. (10) and Zenk et al (20) that elevated sucrose concentration favours alkaloid accumulation in C. ledgeriana and Catharanthus roseus, respectively, and the lack of stimulation by L-tryptophan is in contrast to the report of Hay et al (21) in C. ledgeriana. Possibly, again, these differences reflect the better physiological status of the transformed culture.…”

Section: Discussioncontrasting

confidence: 83%

Quinoline Alkaloid Production by Transformed Cultures ofCinchona ledgeriana

Payne

Rhodes²,

Robins³

1987

Planta Med

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A culture of CINCHONA LEDGERIANA, transformed with AGROBACTERLUM TUMEFACIENS A6 capable of growing and producing quinoline alkaloids in medium free of exogenous phytohormones has been obtained. Unlike the untransformed culture of this species, addition of ZR in combination with either IAA or IBA to medium did not affect alkaloid production. Growing the transformed culture in the dark, however, produced a marked enhancement of alkaloid accumulation, up to 50 times that of cultures grown in the light. This dark-dependent accumulation was not confined to any particular time in the growth cycle, although the extent of the stimulatory effect increased the longer cultures were kept in darkness. Blue light was detrimental to alkaloid accumulation but in red or green light the level of accumulation was equivalent to that in the dark. Alternating cultures for several 28-day periods between light and dark conditions resulted in alternate periods of low and higfralkaloid productivity, indicating this was not an adaption effect. These findings correlate to previously reported differences in key enzymes from the biosynthetic pathway. Increasing the phosphate or nitrate concentration above that of Gamborg B5, increasing sucrose above 2% (w/v), replacing sucrose with glucose or adding tryptophan or casamino acids to the medium resuited in lower alkaloid yields by dark-grown cultures of the transformed line.

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Section: Discussioncontrasting

confidence: 83%

Quinoline Alkaloid Production by Transformed Cultures ofCinchona ledgeriana

Payne

Rhodes²,

Robins³

1987

Planta Med

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“…Hays et a!. (9) reported the incorporation of labelled tryptophan by root organ cultures into quinoline alkaloids. Anderson et a!.…”

Section: Introductionmentioning

confidence: 99%

Effects of Age and Growth Regulators on Growth and Alkaloid Production inCinchona ledgerianaLeaf-Shoot Organ Cultures

Chung

Staba

1987

Planta Med

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CINCHONA LEDGERIANA Moens et Trimen leaf-shoot organ cultures established and maintained on Murashige and Skoog's medium containing benzyladenine (BA 5 mg/l) were used to study the effects of age and growth regulators on alkaloid production. The total and individual alkaloid content of the leaf-shoot organ cultures increased with age and resembled closely that of the 1-year-old plant which favored quinine production. The 32-week-old tissue cultures contained the same amount of alkaloid as that of the 1-year-old plant (350 mg%). Quinine production was favored by the presence of benzyladenine (5 mg/l), gibberellic acid (5 mg/l) and/or shoots. Quinidine production was high in the presence of indole-3-acetic acid (5 mg/l), the absence of benzyladenine, and/or the presence of roots. High concentrations of abscisic acid and mefluidide inhibited growth and alkaloid production.

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“…59 Root cultures incorporated -[methylene-14 C]-27, [2 -14 C]-28 and [C5-3 H]-29 into 36 and 37, confirming the biosynthetic origin of these alkaloids. 60,61 Apparently, the tryptophan supply was limiting, as a five-fold increase in alkaloid occurred in response to exogenous 27. 60…”

Section: Indole Alkaloidsmentioning

confidence: 99%

“…60,61 Apparently, the tryptophan supply was limiting, as a five-fold increase in alkaloid occurred in response to exogenous 27. 60…”

Section: Indole Alkaloidsmentioning

confidence: 99%

The application of root cultures to problems of biological chemistry

Robins¹

1998

Nat. Prod. Rep.

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Introduction 2 The role of root cultures 3 Production, cultivation and properties of root cultures 4 The range of natural products in root cultures 4.1 General aromatic compounds 4.2 Anthraquinones 4.3 Alkaloids 4.3.1 Pyrrolidine alkaloids 4.3.2 Indole alkaloids 4.3.3 Pyrrolizidine alkaloids 4.3.4 Tropane and related alkaloids 4.3.5 Indolizidine alkaloids 4.3.6 Benzylisoquinoline alkaloids 4.4 Terpenoids and steroids 4.4.1 Sesquiterpenes 4.4.2 Diterpenes 4.4.3 Triterpenes and steroids 4.5 Miscellaneous nitrogen compounds 4.6 Miscellaneous sulfur compounds 5 Future prospects 6 References

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In vitro cultures of Cinchona species

Cited by 29 publications

References 13 publications

Quinoline Alkaloid Production by Transformed Cultures ofCinchona ledgeriana

Quinoline Alkaloid Production by Transformed Cultures ofCinchona ledgeriana

Effects of Age and Growth Regulators on Growth and Alkaloid Production inCinchona ledgerianaLeaf-Shoot Organ Cultures

The application of root cultures to problems of biological chemistry

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