In Vitro Cytotoxic Action of Aesculus hippocastanum Extract Against PA 1 Human Ovarian Cell-Line

Abstract

Cancer is not a modern disease but has been present since the very early dawn of life in both animals and humans”. Cancer is a disease that is caused due to alteration in the genetic configuration (mutation) that occurs due to either an inherent biological failure or an externally acquired factor leading to genomic instability and oncogenesis. The sudden upsurge in the incidence of cancer throughout the world in the last two decades has been attributed to the environmental and lifestyle changes which includes smoking, pollution, consumption of processed food etc…It is one among the prominent health problem responsible for morbidity and mortality. It is basically a cell proliferative disorder, initially specific to an organ and advances to invasion and metastasis. Chemotherapy with anticancer drugs remain the standard treatment modality. But those treatment drugs are associated with numerous serious side effects. On the other hand, phytomedicine is now attracting attention due to its potential anticancer properties. These medicines can be considered as an alternative form of therapy in cancer management due to their cost effectiveness, therapeutic efficacy and minimal side effects. Aesculus hippocastanum (AH)commonly known as European horse chestnut is found all over the world. The principal purpose of the study is to explore the anticancer property of ethanolic flower root out of Aesculus hippocastanum by performing the MTT assay (cytotoxic study) against human ovarian cell line. The study was evaluated by assessing the inhibition of percentage of cell viability and it showed minimum viability of cell (53.68 ± 0.8755 %) at the higher concentration of 200µl of Aesculus hippocastanum sample solution with Methotrexate as a standard comparison drug. This has proved the cytotoxic effect of Aesculus hippocastanum on PA1 human ovarian cancer cell line