In Vitro Cytotoxic Activity of Three Egyptian Soybean Cultivars

Abstract

Functional foods play a key role in traditional indigenous medical system that have continued to cure many diseases in developing countries. In order to find new potent source of bioactive principles, the present study was designed to evaluate the cytotoxic effect of the defatted extract from soybean flour of three Egyptian soybean cultivars (Giza 22, Giza 35 and Giza 111). Subsequently, MTT (Methylthiazolyl diphenyl-tetrazolium bromide) assay was used to determine IC50 (half-maximal inhibitory concentration compared with control) of the different extracts against six cancer cell lines, namely HCT-116, EAC, HepG2, CCRF-CEM, PC-3 and MCF-7. The extracts of the three cultivars demonstrated IC50 at values less than 100 µg/mL against all the tested cell lines except CCRF-CEM. Among the studied cell lines, IC50 values below 20 µg/mL were recorded for all extracts against EAC cells. Giza 35 and Giza 111 extracts showed good cytotoxicity against HCT-116 and MCF-7 cells, respectively. Finally, these results provided corroborative information for the possible use of soybean flour as a natural source in the control of cancer disease.