In vitro cytotoxic and anti-herpesvirus properties of jackfruit (Artocarpus heterophyllus Lam., Moraceae) leaf extracts

Sousa, Dhierllate Ferreira de; Fernandes, Maria Judite Bittencourt; Oliveira, Rosilene Aparecida de; Filho, Pedro Costa Campos; Carvalho, Luciana Debortoli de; ConceiÃ§Ã£o, Aline Oliveira da

doi:10.5897/jmpr2019.6845

Cited by 1 publication

(2 citation statements)

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“…20 Leaf extracts of Artocarpus heterophyllus demonstrated an in vitro anti-herpesvirus effect. 21 Curcumin has been confirmed to have antiviral activity against BoHV-1 by impacting the lipid raft formation. 22 Numerous researchers have established the role of plants and their phytoconstituents in the prevention of viral entry, multiplication, and release as well as their immune-boosting nature.…”

Section: ■ Introductionmentioning

confidence: 99%

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Preliminary Evaluation ofLablab purpureusPhytochemicals for Anti-BoHV-1 Activity Using In Vitro and In Silico Approaches

et al. 2023

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Lablab purpureus from the Fabaceae family has been reported to have antiviral properties and used in traditional medical systems like ayurveda and Chinese medicine and has been employed to treat a variety of illnesses including cholera, food poisoning, diarrhea, and phlegmatic diseases. The bovine alphaherpesvirus-1 (BoHV-1) is notorious for causing significant harm to the veterinary and agriculture industries. The removal of the contagious BoHV-1 from host organs, particularly in those reservoir creatures, has required the use of antiviral drugs that target infected cells. This study developed LP-CuO NPs from methanolic crude extracts, and FTIR, SEM, and EDX analyses were used to confirm their formation. SEM analysis revealed that the LP-CuO NPs had a spherical shape with particle sizes between 22 and 30 nm. Energy-dispersive X-ray pattern analysis revealed the presence of only copper and oxide ions. By preventing viral cytopathic effects in the Madin–Darby bovine kidney cell line, the methanolic extract of Lablab purpureus and LP-CuO NPs demonstrated a remarkable dose-dependent anti-BoHV-1 action in vitro. Furthermore, molecular docking and molecular dynamics simulation studies of bio-actives from Lablab purpureus against the BoHV-1 viral envelope glycoprotein disclosed effective interactions between all phytochemicals and the protein, although kievitone was found to have the highest binding affinity, with the greatest number of interactions, which was also validated with molecular dynamics simulation studies. Understanding the chemical reactivity qualities of the four ligands was taken into consideration facilitated by the global and local descriptors, which aimed to predict the chemical reactivity descriptors of the studied molecules through the conceptual DFT methodology, which, along with ADMET finding, support the in vitro and in silico results.

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Section: ■ Introductionmentioning

confidence: 99%