In vitro Cytotoxic and Antimicrobial Activity of Essential Oil From Satureja Intermedia

Abstract

BackgroundMany members of the genus Satureja have aromatic and medicinal characteristics. ObjectivesObjectivesThe purpose of the present work was to determine cytotoxic activity of the essential oil of S. intermedia CA Mey (Lamiaceae) on two human cancerous cell lines and its in vitro inhibitory effects against 11 pathogenic bacteria and fungi as well.Materials and MethodsThe essential oil was isolated by hydrodistillation and analyzed by combination of capillary GC-FID and GC-MS. The in vitro toxicological study was based on the MTT cytotoxicity assay and antimicrobial activity of the essential oil was studied according to the disc diffusion method and MIC value.ResultsThymol (34.5%), γ-terpinene (18.2%) and ρ-cymene (10.5%) were the main components of the essential oil. The toxicological study on 5637 and KYSE cell lines showed IC50 values of 156 μg/ml. The essential oil exhibited considerable antimicrobial activity on tested bacteria and fungi.ConclusionsFrom the results of the present study, it may be concluded that the essential oil of S. intermedia and its major constitutes are interesting in antibacterial and anticancer applications.

A pharmacological and phytochemical overview onSatureja

“…Satureja intermedia is another species in which cytotoxic activity was evaluated by Sadeghi et al (2013). According to data presented in this report, the IC 50 value of the sample against 5637 and KYSE cell lines was found to be 156 lg/mL.…”

Ethnopharmacology, Ecological Requirements, Antioxidant and Antimicrobial activities of Perovskia abrotanoides Karel. Extract for Vaginal Infections From Semnan Province

“…It is being thought that severe military conflicts in Africa shorten the expected lifetime for more than 2 years. In general, WHO had calculated that 269 thousand people had died in 1999 due to the effect of wars and that loss of 8.44 million healthy years of life had occurred (2,3). Wars negatively affect the provision of health services.…”

“…Moreover, the wars cause the migration of qualified health employees, and thus the health services hitches. Assessments made indicate that the effect of destruction in the infrastructure of health continues for 5-10 years even after the finalization of conflicts (3). Due to resource requirements in the restructuring investments after war, the share allocated to health has decreased (1).…”

“…In confirmation of our data, many similar researches have shown that the antioxidant and antimicrobial activities of other plant species such as P. abrotanoides, M. longifolia, Thymus spp., A. annua, A. sieberi, J. sabina, P. harmala, and Punica granatum have been previously studied and many of them have reported that the antimicrobial activity of essential oil and the extracts, relative to their main phenolic components (thymol, menthol, carvacrol, γ-terpinene terpinene 4-ol and p-cymene), has shown a high inhibitory effect against a wide range of microorganisms (3,11,(18)(19)(20).…”

Cytotoxicity, Radical Scavenging, Antioxidant Properties and Chemical Composition of the Essential Oil of Satureja cilicica P.H. Davis from Turkey

“…Satureja species are usually subject of antibacterial, antifungal, antioxidant, cytotoxicity, antidiabetes, anti-HIV, anti-hyperlipidemic, reproduction stimulatory, expectorant and vasodilatory activity studies (5)(6)(7)(8).…”