In vitro Cytotoxic Effect, Hemolytic, and Antioxidant Activities of the Algerian Species Nonea vesicaria Rchb.

Abstract

Objective: The aim of this study is the estimation of total phenolic and flavonoid contents and the evaluation of cytotoxic, hemolytic and antioxidant activities of the methanolic extract obtained from the species Nonea vesicaria (L.) Rchb. Methods: The total phenolic and flavonoid contents were quantified by Folin-Ciocalteu and trichloroaluminum methods respectively. The cytotoxic effect was tested by Brine shrimp lethality assay and the hemolytic activity was assessed by spectrophotometric test on human erythrocytes. Moreover, the antioxidant activity was determined by seven different technics. Results: The phytochemical screening revealed the presence of many classes of secondary metabolites, a moderate level of polyphenols and a low content of flavonoids. The methanolic extract showed a significant cytotoxic effect with a value of LC50 at 35.7 ± 0.5 µg/mL and induced hemolysis in a dose-dependent manner with a value of EC50 at 175.6 ± 0.08 μg/mL. The results of antioxidant activities indicated an important effect in nonpolar systems especially in ferric thiocyanate test and β-carotene bleaching inhibition assay. Conclusion: The methanolic extract of N. vesicaria could constitute an important source of antioxidant and cytotoxic compounds but a prudent use is recommended in order to reduce the adverse effects related to the possible hemolysis.

Investigation of Photoprotective, Anti-Inflammatory, Antioxidant Capacities and LC–ESI–MS Phenolic Profile of Astragalus gombiformis Pomel

“…Brine shrimp bioassay was used to elucidate the possible toxicity of bioactive compounds in extracts which are generally toxic in high doses [75]. According to Mouffouk et al (2020) [76], the secondary metabolites present in Noneavesicaria extract induced direct damage on membrane integrity by causing cell lysis. Therefore, these compounds may likely have cytotoxic effects, something that may also apply to A. gombiformis.…”

Microwave-Assisted Extraction and Phytochemical Profile of Nonea pulmonarioides and Its Antifungal, Antibacterial, and Antioxidant Activities

“…ese plants contain phytochemicals mainly known for biological activity and the treatment of cancer, oxidation, and neurological diseases and have fewer side effects than synthetic products. In the current study, the phytochemicals in N. pulmonarioides have more important biological activity and can be useful for treating other diseases [7,33]. e phytochemical results from the petroleum ether, acetone, and methanol extracts prepared from N. pulmonarioides indicated the presence of some secondary metabolic products.…”

“…Furthermore, compared to the methanolic extract of N. pulmonarioides, the species N. melanocarpa has a lower concentration of polyphenols (22.2 μg/mg) [38]. Additionally, the rate of flavonoids in the methanol extract in N. pulmonarioides is higher than in N. vesicaria [7]. e TPC and TFC vary between species of the same genus due to various biotic and abiotic factors, such as the genetic potential, environmental conditions, and storage period.…”

“…In developing countries, a very high number of people depend on traditional medicinal plants for their primary health care [6]. In addition, medicinal plant researchers are interested in the most important parts of plants, the bioactive compounds [7]. Recently, many researchers have focused on phytochemical studies to find the bioactive compounds used in medicine [8].…”

The complete plastome of Nonea vesicaria (L.) Rchb. (Boraginaceae), the first chloroplast genome belonging to the Nonea genus

“…Despite harboring stearidonic acid-rich seeds (an omega-3 fatty acid) and being a source of gamma-linolenic acid used for premenstrual syndrome, arthritis, blood pressure, and skin disorders, N. vesicaria extracts have been described as having cytotoxic, hemolytic, and antioxidant activities (Mouffouk et al. 2020 ), and as such should be carefully monitored.…”