In vitro cytotoxic effects of Smilax aspera L. roots on cancer cell lines

Dalkılıç, Semih; Korkmaz, İsmail; Dalkılıç, Lütfiye Kadıoğlu; Akay, Gökhan; Fidan, Songül

doi:10.1016/j.fbio.2021.101501

Cited by 7 publications

(4 citation statements)

References 64 publications

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“…Apoptosis and cytotoxicity in human osteosarcoma (U2OS) cells have been demonstrated in Smilax aspera , one of the species of this genus ( 40 ). The same plant was associated with cytotoxic effect on lung cancer cells ( 41 ) as well as on ovarian adenocarcinoma (OVCAR3), lung carcinoma (A549) and breast cancer (MDA-MB-231) cell lines ( 42 ).…”

Section: Resultsmentioning

confidence: 99%

Total Antioxidant Potential, Total Phenolic Profile and Cytotoxic Activity Against Brain Cancer: Melocan and Galdirik

Al Yassine,

El Massri,

Demircan

et al. 2023

Food Technol. Biotechnol. (Online)

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Research background. Brain cancer is known to be one of the most difficult types of cancer to be cure. It has serious impact on the lives of diagnosed people due to the insufficient treatment options and their side effects. Thus, the search for novel alternative treatments is ongoing. Melocan (Smilax excelsa L.) and Galdirik (Trachystemon orientalis) are significant contributors to both traditional culinary culture and traditional medicine around Black Sea; however, the evidence regarding their antioxidative and cytotoxic effects remains fairly limited. Experimental approach. This study aimed to determine the antioxidant and cytotoxic activity of Smilax excelsa and Trachystemon orientalis, on the C6 glioblastoma cell line. Smilax excelsa and Trachystemon orientalis plants were dried and extracted; then, the total phenolic content (TPC) and their phenolic profiles were studied. In addition, their Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) were determined by using an assay kit. We also analyzed the total antioxidant activity (TAA) using the DPPH radical scavenging assay and the cytotoxic impact on the glioma cells via the 3-(4,5-dimethylthiazol-2 yl)-2,5-diphenyltetrazolium (MTT) assay. Results and conclusions. According to the results, the water extracts of both Smilax excelsa (1158.17 mg gallic acid eq./100 g DM) and Trachystemon orientalis (262 mg gallic acid eq./0 g DM) had higher TPC than the ethanol extracts. TAA were measured as 192.86 mg and 131.92 Trolox eq./100 g DM for the Smilax excels and Trachystemon orientalis, respectively. The MTT assay revealed that Trachystemon orientalis had a greater cytotoxic effect. In conclusion, the findings of the current study are promising. Novelty and scientific contribution. This is the first study that aimed to evaluate the potential cytotoxic activity of two local Turkish plants, Smilax excelsa and Trachystemon orientalis, against C6 glioblastoma cells and the findings ensure that both plants may be used in the future as good therapeutic agents for treating cancer.

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Section: Resultsmentioning

confidence: 99%

Total Antioxidant Potential, Total Phenolic Profile and Cytotoxic Activity Against Brain Cancer: Melocan and Galdirik

Al Yassine,

El Massri,

Demircan

et al. 2023

Food Technol. Biotechnol. (Online)

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“…After incubation, the medium was removed and formazan crystals were dissolved in 100 μl DMSO. The expected color change was then determined by measuring absorbance at 492 nm wavelength using an ELISA (Enzyme Linked ImmunoSorbent Assay) microplate reader [22].…”

Section: Mtt Assaymentioning

confidence: 99%

Investigation of Antioxidant, Antimicrobial and Cytotoxic Activity of Cydonia oblonga Leaf on Breast Cancer (MCF-7) and Liver Cancer (HepG2) Cell Lines

Kadıoğlu Dalkılıç

2024

Turkish Journal of Science and Technology

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Dünyadaki en yaygın patolojilerden biri olan kanser, yüzyıllardır birçok insanın yaşam standartlarını düşürmekle beraber ölümüne neden olabilmektedir. Vücutta normal hücrelerin kontrolsüz ve anormal bir şekilde büyümesi sonucu ortaya çıkan bir hastalık olmakla beraber çevre dokulara zarar vererek metastaz oluşturabilmektedir. Kanser tedavisinde birçok tıbbi bitki, hastalık semptomlarını iyileştirebilme özellikleri ve bununla birlikte düşük yan etkileri nedeniyle yaygın olarak kullanılmaktadır. Ayrıca bu bitkilerin çeşitli aktif bileşikleri antiviral, antikanser, antibakteriyel, antifungal, antiinflamatuar ve antioksidan gibi özelliklere sahip olduğu bilinmektedir. Cydonia oblonga, halk arasında ayva olarak bilinen bir bitki türüdür. Asya kökenli olmakla beraber günümüzde birçok ülkede yetiştirilmektedir. Bu çalışmada, toplanan C. oblonga yaprağına metanol ve hekzan çözücülerinde ekstraksiyon işlemi uygulanmıştır. Çıkarılan ekstreler, MCF-7 ve HepG2 hücre hatları üzerinde MTT Assay yöntemi kullanılarak sitotoksik aktivitesi, DPPH yöntemi kullanılarak antioksidan aktivitesi tespit edilmiş ve ekstraktın beş ayrı mikroorganizma üzerinde kuyu agar yöntemi ile antimikrobiyal etkileri belirlenmiştir. C. oblonga hekzan ekstraktının metanol ekstraktına göre daha güçlü sitotoksik aktivite sergilediği görülmekle beraber metanol ekstraktının, hekzana göre daha güçlü antioksidan etkisi olduğu belirlenmiştir. Mikroorganizmalara uygulanan ekstreler en iyi zon çapı etkisini E. coli üzerinde gösterirken en düşük etkiyi B. megaterium üzerinde göstermiştir. Bunlarla birlikte, C. oblonga yaprağının antikanser ve antioksidan aktivitesinden daha güçlü antimikrobiyal etkilere sahip olduğu gözlemlenmiştir.

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“…The extraction was repeated 4-6 times using a Rotary evaporator at 40 °C. The obtained extract was then dissolved in DMSO and prepared at four different concentrations (250, 500, 750, and 1000 µg/ml) (Dalkılıç et al, 2022). The steps of the experiment and synergistically effective extracts are shown in (Table 1 and Figure 1).…”

Section: Sample Retrieval and Extract Preparationmentioning

confidence: 99%

“…The MCF-7 cell line was cultured in RPMI with 2 mM L-Glutamine, 1% Penicillin-Streptomycin, and 10% Fetal Bovine Serum in 25/75 cm 2 flasks at 37 ± 0.1 °C and 5% CO2. The cytotoxic activity of the substances was determined by measuring the effect of the substances on the viability of these cell lines (Dalkılıç et al, 2022). After the MDA-MB-231 cells grown in flasks became confluent, the medium was removed and washed with 5 ml of PBS solution.…”

Section: Cytotoxic Activitymentioning

confidence: 99%

Determination of cytotoxic, apoptotic, necrotic, antimicrobial and antioxidant activities of Aloe vera and Abies cilicia subsp. cilicica

Dalkılıç,

Küçüktüfekçi

et al. 2023

ijpbp

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Cancer is one of the most common pathologies in the world, leading to a reduced standard of living and even death for centuries. Despite promising developments in treatment methods in recent years, the expected level of treatment and success hasn't yet been achieved due to the side effects and cost of treatment methods and the fact that some drugs are still in the trial phase. This situation has encouraged the scientific community to search for natural agents with lower costs and limited side effects. Abies cilicica, also known as fir, and Aloe vera has been used in both food and traditional medicine from the past to the present. In the literature review, it was found that both A. vera and A. cilicica have many beneficial effects, especially anti-inflammatory, antifungal, and wound-healing properties. This study aimed to investigate the antimicrobial, antioxidant, cytotoxic, and apoptotic/necrotic effects of extracts of A. vera from Asphodelaceae and A. cilicica (Ant. Et Kotschy.) subsp. cilicica Carr. from Pinaceae. The best antimicrobial activity was observed against Escerichia coli with a zone diameter of 20.00 ± 3.59 mm and Klebsiella pneumoniae with a zone diameter of 21 ± 5.35 mm. 2KA showed the best effect on antioxidant activity. 2MA + 2KSA showed significant cytotoxic activity on MDA-MB-231 cancer cells. IC50 values of 1EA + 1KA extract (whole A. cilicica and A. vera dissolved in ethanol) against MDA-MB-231 cell line (IC50 458.29 ± 19.01 µg/ml) and MCF-7 cell line (IC50 596.03 ± 5.56 µg/ml) were determined. According to the data obtained from the study, A. vera and A. cilicica were found to have antimicrobial, antioxidant, and cytotoxic effects both alone and synergistically. It is predicted that they can be used especially in cancer treatment.

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In vitro cytotoxic effects of Smilax aspera L. roots on cancer cell lines

Cited by 7 publications

References 64 publications

Total Antioxidant Potential, Total Phenolic Profile and Cytotoxic Activity Against Brain Cancer: Melocan and Galdirik

Total Antioxidant Potential, Total Phenolic Profile and Cytotoxic Activity Against Brain Cancer: Melocan and Galdirik

Investigation of Antioxidant, Antimicrobial and Cytotoxic Activity of Cydonia oblonga Leaf on Breast Cancer (MCF-7) and Liver Cancer (HepG2) Cell Lines

Determination of cytotoxic, apoptotic, necrotic, antimicrobial and antioxidant activities of Aloe vera and Abies cilicia subsp. cilicica

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