1986
DOI: 10.1002/jnr.490160408
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In vitro cytotoxicity and demyelination induced by theiler viruses in cultures of spinal cord slices

Abstract: The cytopathic effects caused by Theiler viruses to myelinated organotypic spinal cord cultures was studied by light and electron microscopy. Heavily myelinated cultures, 2-3 weeks in vitro were infected with WW and GD VII viruses. Mock infection served as control. On light microscopy cytopathic effects and demyelination became evident about 16-17 hr after infection. Demyelination observed in WW virus-infected cultures was much more pronounced than in cultures infected with GD VII viruses. The myelin in mock-i… Show more

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Cited by 10 publications
(7 citation statements)
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“…Thus, WW virus can infect oligodendrocytes and produce demyelination in “immune-free” CNS organotypic cultures. Similarly, demyelination and axonal degeneration were observed in a spinal cord slice culture, 17 h after WW or GDVII infection, while demyelination in the WW virus-infected cultures was more pronounced than in cultures infected with GDVII virus (Shahar et al 1986). In vitro studies can mimic many aspects of TMEV infection, such as persistent infection in the mouse macrophage RAW264.7 cell line (Steurbaut et al 2006).…”
Section: Role Of Immune Responsesmentioning
confidence: 76%
“…Thus, WW virus can infect oligodendrocytes and produce demyelination in “immune-free” CNS organotypic cultures. Similarly, demyelination and axonal degeneration were observed in a spinal cord slice culture, 17 h after WW or GDVII infection, while demyelination in the WW virus-infected cultures was more pronounced than in cultures infected with GDVII virus (Shahar et al 1986). In vitro studies can mimic many aspects of TMEV infection, such as persistent infection in the mouse macrophage RAW264.7 cell line (Steurbaut et al 2006).…”
Section: Role Of Immune Responsesmentioning
confidence: 76%
“…In distinction from WNV, Theiler's virus attacks the myelin-forming cells and replicates in them, leading to the displacement of the myelin sheath and to a massive demyelination (Frankel et al, , 1987Shahar et al, 1986).…”
Section: Discussionmentioning
confidence: 98%
“…Anti-MOG antibody in combination with complement demyelinates mouse cerebellar slices as shown by loss of MBP-positive immunofluorescence and ELISA specific for MBP (Harrer et al, 2009). Theiler virus added to myelinated mouse spinal cord cultures caused marked demyelination (Shahar et al, 1986). In 2004, Birgbauer et al developed a system of demyelinating rat cerebellar slices put into culture at P10 and demyelinated after 1 week using addition of lysolecithin to the culture medium for 15-17 h, with the subsequent return of myelin sheaths seen by immunofluorescence visible around 1 week later (Birgbauer et al, 2004).…”
Section: Demyelinationmentioning
confidence: 98%