In vitro cytotoxicity andin vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) hydrogels

Suggs, Laura J.; Shive, Matthew S.; García, Celsa; Anderson, James M.; Mikos, Antonios G.

doi:10.1002/(sici)1097-4636(199907)46:1<22::aid-jbm3>3.0.co;2-r

Cited by 121 publications

(84 citation statements)

References 22 publications

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“…Implants were performed as described (9,10). Briefly, surfaces were placed in surgical-grade stainless steel wire mesh cages (1.5 cm in length, 0.5 cm in diameter, 0.25-mm wire diameter, and 0.8-mm opening width with 58% open area) and sterilized with ethylene oxide.…”

Section: Methodsmentioning

confidence: 99%

“…One half of the exudate was processed for total leukocyte and differential cell counting (described in ref. 10), and the other half was stained for apoptosis markers for analysis by flow cytometry. Collected cells were pelleted and washed twice before staining with annexin V-FITC and propidium iodide (R & D Systems) according to manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Biomaterial adherent macrophage apoptosis is increased by hydrophilic and anionic substratesinvivo

Brodbeck

Patel

Voskerician

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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223

166

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An in vivo rat cage implant system was used to identify potential surface chemistries that prevent failure of implanted biomedical devices and prostheses by limiting monocyte adhesion and macrophage fusion into foreign-body giant cells while inducing adherent-macrophage apoptosis. Hydrophobic, hydrophilic, anionic, and cationic surfaces were used for implantation. Analysis of the exudate surrounding the materials revealed no differences between surfaces in the types or levels of cells present. Conversely, the proportion of adherent cells undergoing apoptosis was increased significantly on anionic and hydrophilic surfaces (46 ؎ 3.7 and 57 ؎ 5.0%, respectively) when compared with the polyethylene terephthalate base surface. Additionally, hydrophilic and anionic substrates provided decreased rates of monocyte͞macro-phage adhesion and fusion. These studies demonstrate that biomaterial-adherent cells undergo material-dependent apoptosis in vivo, rendering potentially harmful macrophages nonfunctional while the surrounding environment of the implant remains unaffected.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Biomaterial adherent macrophage apoptosis is increased by hydrophilic and anionic substratesinvivo

Brodbeck

Patel

Voskerician

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

223

166

View full text Add to dashboard Cite

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“…A v ariety of injectable materials, both ceramic-and polymer-based, have been developed for use in multiple orthopaedic applications [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. The combination of ceramic particles with polymeric matrices has also been extensiv ely inv estigated, in an attempt to mimic bone tissue, which may itself be seen as a complex composite material made of organic and inorganic components.…”

Section: Introductionmentioning

confidence: 99%

“…The combination of ceramic particles with polymeric matrices has also been extensiv ely inv estigated, in an attempt to mimic bone tissue, which may itself be seen as a complex composite material made of organic and inorganic components. Different ceramic phases have been used, hydroxyapatite and tricalcium phosphate being the most common [4][5][6][7][8][9], as well as several polymeric matrices, both from synthetic [10][11][12][13][14] or natural origin, the latter including collagen, chitosan, gelatine and alginate, among others [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Calcium phosphate-alginate microspheres as enzyme delivery matrices

2004

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The present study concerns the preparation and initial characterisation of nov el calcium titanium phosphate-alginate (CTPalginate) and hydroxyapatite-alginate (HAp-alginate) microspheres, which are intended to be used as enzyme delivery matrices and bone regeneration templates. Microspheres were prepared using different concentrations of polymer solution (1% and 3% w/v) and different ceramic-to-polymer solution ratios (0.1, 0.2 and 0.4 w/w). Ceramic powders were characterised using X-ray diffraction, laser granulometry, Brunauer, Emmel and Teller (BET) method for the determination of surface area, zeta potential and Fourier transform infrared spectroscopy (FT-IR). Alginate was characterised using high performance size exclusion chromatography. The methodology followed in this investigation enabled the preparation of homogeneous microspheres with a uniform size. Studies on the immobilisation and release of the therapeutic enzyme glucocerebrosidase, employed in the treatment of Gaucher disease, were also performed. The enzyme was incorporated into the ceramic-alginate matrix before gel formation in two different ways: preadsorbed onto the ceramic particles or dispersed in the polymeric matrix. The two strategies resulted in distinct release profiles. Slow release was obtained after adsorption of the enzyme to the ceramic powders, prior to preparation of the microspheres. An initial fast release was achiev ed when the enzyme and the ceramic particles were dispersed in the alginate solution before producing the microspheres. The latter profile is very similar to that of alginate microspheres. The different patterns of enzyme release increase the range of possible applications of the system inv estigated in this work.

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“…Studies have shown that materials exhibiting adverse in vitro behaviour may still show appropriate results in vivo [33,34]. The differences between in vitro and in vivo studies may be due to higher localised concentrations of cytotoxic or leachable products in vitro versus a more diluted case in an implant [33]. Figure 5 shows the cumulative release profile of heparin from PUNC coils.…”

Section: Cell Growth Inhibition (Cgi)mentioning

confidence: 99%

Polyurethane Organosilicate Nanocomposites as Blood Compatible Coatings

et al. 2012

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Polymer clay nanocomposites (NCs) show remarkable potential in the field of drug delivery due to their enhanced barrier properties. It is hypothesised that well dispersed clay particles within the polymer matrix create a tortuous pathway for diffusing therapeutic molecules, thereby resulting in more sustained release of the drug. As coatings for medical devices, these materials can simultaneously modulate drug release and improve the mechanical performance of an existing polymer system without introducing additional materials with new chemistries that can lead to regulatory concerns. In this study, polyurethane organosilicate nanocomposites (PUNCs) coated onto stainless steel wires were evaluated for their feasibility as blood compatible coatings and as drug delivery systems. Heparin was selected as the model drug to examine the impact of silicate loading and modifier chain length in modulating release. Findings revealed that better dispersion was achieved from samples with lower clay loadings and longer alkyl chains. The blood compatibility of PUNCs as assessed by thrombin generation assays showed that the addition of silicate particles did not significantly decrease the thrombin generation lag time (TGT, p = 0.659) or the peak thrombin (p = 0.999) of polyurethane (PU). PUNC coatings fabricated in this research were not cytotoxic as examined by the cell growth inhibition OPEN ACCESSCoatings 2012, 2 46 assay and were uniformly intact, but had slightly higher growth inhibition compared to PU possibly due to the presence of organic modifiers (OM). The addition of heparin into PUNCs prolonged the TGT, indicating that heparin was still active after the coating process. Cumulative heparin release profiles showed that the majority of heparin released was from loosely attached residues on the surface of coils. The addition of heparin further prolonged the TGT as compared to coatings without added heparin, but a slight decrease in heparin activity was observed in the NCs. This was thought to be from competitive interactions between clay-heparin that influenced the formation of the ternary complex between heparin, ATIII thrombin. In summary, the feasibility of using PUNC as drug delivery coatings was shown by the good uniformity in the coating, absence of by-products from the coating process, and the release of active molecules without significantly interfering with their activity.

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In vitro cytotoxicity andin vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) hydrogels

Cited by 121 publications

References 22 publications

Biomaterial adherent macrophage apoptosis is increased by hydrophilic and anionic substratesinvivo

Biomaterial adherent macrophage apoptosis is increased by hydrophilic and anionic substratesinvivo

Calcium phosphate-alginate microspheres as enzyme delivery matrices

Polyurethane Organosilicate Nanocomposites as Blood Compatible Coatings

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