In Vitro Cytotoxicity Evaluation of Novel Nano-Hydroxyapatite-Silica Incorporated Glass Ionomer Cement

Abstract

Introduction: Glass Ionomer Cements (GIC) are among the most popular restorative materials, but their use in dentistry is limited due to their physical properties. The hardness of GIC was improved by incorporation of nano-hydroxyapatite-silica into GIC, to expand its applicability. Aim:To evaluate the cytotoxic effects of nano-hydroxyapatitesilica incorporated glass ionomer cement (HA-SiO 2 -GIC) on human Dental Pulp Stem Cells (DPSC) and compare it with conventional GIC and resin modified GIC. Materials and Methods:Material extracts of Fuji IX, Fuji II LC and HA-SiO 2 -GIC were prepared into seven serial concentrations and applied to 96-well-plates seeded with DPSC. The 96-wellplates were incubated for 24 and 72 hours. The morphology of DPSC was observed under the inverted phase contrast microscope, and the cell viability was determined using MTT assay at both time intervals. Kruskal-Wallis test was performed for statistical analysis. Results:At maximum concentration, DPSC appeared fewer in number, but the normal spindle morphology was maintained in all groups except for Fuji II LC. At lower concentrations, DPSC appeared normal and more confluent in all groups. The cytotoxic effects of all groups were dose dependent. Fuji IX demonstrated the lowest cytotoxicity, followed by HA-SiO 2 -GIC. Fuji II LC demonstrated the highest cytotoxicity. The difference was significant between all groups at 200 mg/ml concentration (p<0.05). At concentration <100 mg/ml, cytotoxicity of HA-SiO 2 -GIC was comparable to that of Fuji IX and lower than that of Fuji II LC. Conclusion:HA-SiO 2 -GIC showed a favourable cytotoxicity response and thus holds promise as a future potential restorative material in clinical dentistry.

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

“…These results are probably related to the composition. Bioactive materials promote Ca 2+ ion release, which has mitogenic effects on mesenchymal cells [24]. In contrast, Ionolux was cytotoxic.…”

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

“…Most common formazan salts used to assess cell viability are MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide), WST-1, and WST-8 reagents [56,57,58,59,60]. The MTT/MTS test is widely used to assess the biocompatibility of CaPs [52,61,62,63]. Using the MTS method, Santos et al [52] showed that both chemically and hydrothermally synthesized HA did not affect the viability of MG63 cells treated for three and six days with HA concentrations lower than 500 μg/mL.…”

Glass Ionomer Cement Modified by Resin with Incorporation of Nanohydroxyapatite: In Vitro Evaluation of Physical-Biological Properties

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