In Vitro Cytotoxicity of GuttaFlow 2 on Human Gingival Fibroblasts

Mandal, Preeti; Zhao, Jiajia; Sah, Sanjay Kumar; Huang, Yuting; Liu, Jiarong

doi:10.1016/j.joen.2014.01.025

Cited by 26 publications

(24 citation statements)

References 33 publications

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“…Nonetheless, the material showed no disadvantages in comparison to the other sealers tested and therefore it may be considered suitable to fill the root canal system. Moreover, recent studies have shown that GuttaFlow 2 is slightly or non-cytotoxic 10,11 and presents good sealing ability 12 . To date, there are no other reported studies evaluating the flow of GuttaFlow 2 into lateral or secondary canals.…”

Section: Discussionmentioning

confidence: 99%

“…According to the manufacturer, this new endodontic sealer presents good physicochemical and antimicrobial properties 9 . However, it has been poorly investigated [10][11][12] and it should be further compared with existing materials. The AH Plus (DeTrey Dentsply GmbH, Konstanz, Germany) is based on epoxy resin and it is considered a goldstandard material for root filling due to its long history of clinical success 6 and remarkable bond strength to dentin 13 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effectiveness of lateral and secondary canal filling with different endodontic sealers and obturation techniques

Teixeira¹,

Silva²,

Waltrick³

et al. 2017

Rev. da Fac. de Odontologia, UPF

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effectiveness of lateral and secondary canal filling with different endodontic sealers and obturation techniques

Teixeira¹,

Silva²,

Waltrick³

et al. 2017

Rev. da Fac. de Odontologia, UPF

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“…Several methods have been introduced for cytotoxicity testing of dental materials, including: the methyl-thiazol-tetrazolium (MTT) assay, CCK-8 assay, florescent dyes and flow cytometry [20,21] . In the present study, CCK-8 assay was chosen as a common technique for evaluating the cytotoxicity of modified nonequilibrium plasma with 2% CHX on HGFs.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Chen

Shi

Qing

et al. 2016

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The aim of this study was to investigate the cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate (CHX) on human gingival fibroblasts (HGFs), and to evaluate the biosecurity of modified nonequilibrium plasma with 2% CHX as a new method of root canal treatment. Tissue samples taken from human gingiva were primarily cultured and passaged. Cells from passages 3-7 were used. HGFs were treated by modified nonequilibrium plasma with 2% CHX for 0 min (control group), 30 s, 1 min, 1.5 min, 3 min, 5 min, and 10 min, respectively, and then they were incubated for 0, 24, and 48 h. After that, cell counting kit-8 (CCK-8) assay was applied to analyze the cytotoxicity of modified nonequilibrium plasma with 2% CHX on HGFs. There was no significant difference between the 0 h group treated with the modified nonequilibrium plasma for 1 min and the control group (P>0.05). However, there were significant differences between all the other treated groups and the control group (P<0.05). When treated for 1.5 min or shorter, the cell viability was obviously increased; while treated for 3 min or longer, it was obviously reduced. Moreover, when successively cultured for 0, 24, and 48 h, cell viability was decreased at first and then increased in the 3-min-treated and 5-min-treated groups. The modified nonequilibrium plasma with 2% CHX was of no influence on cell viability in 1.5 min treatment, and it could be safely used on root canal treatment.

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“…The evaluation of GuttaFlow new formulation is necessary since the biological effects of silver particles depends on its size and surface that are in contact with tissues (13)(14). As a matter of fact, it was found that silver microparticles present a smaller number of atoms in their surface comparing to silver nanoparticles (13)(14), which resulted in reduced in vitro toxicity (15). Thus, it was possible that particles with different size presented better biological responses in contact with connective tissue.…”

Section: Introductionmentioning

confidence: 99%

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

et al. 2016

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Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another. Tissue samples with implants were processed for histological analysis after 7 or 60 days (n=6 animals per period). Inflammatory cells, fibrous condensation and abscess were scored according to their intensity. Friedman, followed by Dunn's post hoc, was used to compare sealers. Differences between the two experimental periods were verified using Mann-Witney U test (p<0.05). At 7 days, most of the histological parameters showed no significant differences amongst groups. Endofill group scored higher than the others for giant cells (o<0.05) and promoted a greater number of samples presenting abscess formation. GuttaFlow 2 tended to show a less intense inflammatory infiltrate compared to the other materials. At 60 days, there were no significant differences between groups in most of the histological parameters evaluated. However, it was observed that Endofill scored higher for macrophages (p<0.05) compared to the control group, and GuttaFlow 2 tended to present lower scores than the others for neutrophils and abscess. GuttaFlow 2 showed proper biological behavior and should be considered adequate for clinical practice.

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In Vitro Cytotoxicity of GuttaFlow 2 on Human Gingival Fibroblasts

Cited by 26 publications

References 33 publications

Effectiveness of lateral and secondary canal filling with different endodontic sealers and obturation techniques

Effectiveness of lateral and secondary canal filling with different endodontic sealers and obturation techniques

Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

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