In Vitro Cytotoxicity of Native and Rec-Pediocin CP2 Against Cancer Cell Lines: A Comparative Study

Abstract

Pediocin CP2 is a natural antimicrobial peptide produced by Pediococcus acidilactici MTCC 5101. Its recombinant version was created by employing computational protein engineering approach and was expressed as a synthetic fusion protein in recombinant E. coli BL21(DE3)-pedA. Both native and rec-pediocin were comparatively evaluated for their cytotoxicity against HepG2 (a hepatocarcinoma cell line), HeLa (a cervical adenocarcinoma), MCF7 (a mammary gland adenocarcinoma) and Sp2/0-Ag14 (a spleen lymphoblast) cell lines. Inhibition of cell proliferation was quantitated by MTT assay and induction of apoptosis was studied by genomic DNA fragmentation assay. Results indicated a significantly higher cytotoxicity of rec-pediocin and damage of chromosomal DNA in bacteriocin tested cell lines.

Biomedical Applications of Fermenticin HV6b Isolated fromLactobacillus fermentumHV6b MTCC10770

“…The results presented here indicate cytotoxic effect of fermenticin HV6b on various cancerous cell lines. The cytotoxic effect on cancerous cells from human origin was also reported earlier [27, 36]. The uniqueness of the bacteriocins lies in their interaction with the cell surface without penetrating the target cells, yet affecting cell division and DNA synthesis [37].…”

“…The MTT assay was carried out according to the protocol given by Kumar et al [27]. The viability of the cells exposed to bacteriocin was measured as a direct proportion of the breakdown of yellow compound tetrazolium to dark blue water insoluble formazan.…”

“…It was carried out as per methodology of Kumar et al [27] where cells (1 × 10 5 ) were treated with 1.0 mg/mL fermenticin HV6b for 48 h and then lysed with 250  μ L lysis buffer. After incubation at 37°C for 90 min, 200  μ g/mL proteinase K and lithium chloride (0.2% w/v) were added and incubated again for 60 min at 50°C.…”

In VitroandIn VivoSurvival and Colonic Adhesion ofPediococcus acidilacticiMTCC5101 in Human Gut

“…Cells were routinely grown in Dulbecco modified Eagle's minimal essential medium containing 25 mM glucose, 10% fetal bovine serum, and 1% antibiotic solution containing penicillin and streptomycin (Himedia) [15]. …”

“…Pediocin CP2 exhibits a wide range of antimicrobial activity against Gram-positive, Gram-negative bacteria as well as fungi [12]. Genes encoding production of Pediocin CP2 are localized to the ped operon present on the 8.9 kb plasmid pCP289 of P. acidilactici MTCC5101 [13–15]. These properties make this sp.…”