In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells

Kim, Jung Eun; Kim, Hye-Jin; An, Seong Soo A.; Maeng, Eun Ho; Kim, Meyoung Kon; Song, Young-Bo

doi:10.2147/ijn.s57936

Cited by 17 publications

(7 citation statements)

References 39 publications

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“…In contrast, 200 nm aSiNPs did not show any toxicity even at relatively high concentrations. Kim et al 66 determined the cytotoxicity of aSiNPs with mean sizes of either 20 nm or 100 nm in human glioblastoma cell line (U373MG). The results indicated that the 20 nm nanoparticles were more toxic than the 100 nm nanoparticles.…”

Section: Size-dependent Cytotoxicity Of Asinps On Cell Lines From Kidmentioning

confidence: 99%

<p>The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies</p>

Dong¹,

Wu²,

Li³

et al. 2020

IJN

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Section: Size-dependent Cytotoxicity Of Asinps On Cell Lines From Kidmentioning

confidence: 99%

<p>The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies</p>

Dong¹,

Wu²,

Li³

et al. 2020

IJN

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“…This observation is consistent with the previously published work where neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. 53 However, we do not exclude the possibility that the short length of CNCs derivative may contribute to less nanomaterial agglomeration or cell clustering formation around the CNCs and therefore better distribution onto the cell surface, resulting in less cytotoxicity.…”

Section: Resultsmentioning

confidence: 93%

Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines

Jimenez

Jaramillo

Hemraz

et al. 2017

NSA

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Cellulose nanocrystals (CNCs) have emerged as promising candidates for a number of bio-applications. Surface modification of CNCs continues to gain significant research interest as it imparts new properties to the surface of the nanocrystals for the design of multifunctional CNCs-based materials. A small chemical surface modification can potentially lead to drastic behavioral changes of cell-material interactions thereby affecting the intended bio-application. In this work, unmodified CNCs were covalently decorated with four different organic moieties such as a diaminobutane fragment, a cyclic oligosaccharide (β-cyclodextrin), a thermoresponsive polymer (poly[N-isopropylacrylamide]), and a cationic aminomethacrylamide-based polymer using different synthetic covalent methods. The effect of surface coatings of CNCs and the respective dose-response of the above organic moieties on the cell viability were evaluated on mammalian cell cultures (J774A.1 and MFC-7), using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide and lactate dehydrogenase assays. Overall, the results indicated that cells exposed to surface-coated CNCs for 24 h did not display major changes in cell viability, membrane permeability as well as cell morphology. However, with longer exposure, all these parameters were somewhat affected, which appears not to be correlated with either anionic or cationic surface coatings of CNCs used in this study.

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“…While charge is thought to be a highly influential factor in determining particle internalization, little consensus has been achieved to determine whether positive, negative or neutral charge facilitates or hinders particle internalization (Ayala et al, 2013; Bahmani et al, 2011; Harush-Frenkel et al, 2007; He et al, 2010; Kim et al, 2014; Wilhelm et al, 2003). Part of the difficulty in reaching consensus is the lack of consistency among testing conditions for various studies: chemistry of bead production, cell culture conditions and in vivo techniques are highly variable and can yield vastly different results for any given particle and cell type.…”

Section: Discussionmentioning

confidence: 99%

Neurons Internalize Functionalized Micron-Sized Silicon Dioxide Microspheres

Wallace¹,

Cimbro

Rubio³

et al. 2017

Cell Mol Neurobiol

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Microparticles have potential as neuron-specific delivery platforms and devices with many applications in neuroscience, pharmacology and biomedicine. To date, most literature suggests that neurons are not phagocytic cells capable of internalizing microparticles larger than 0.5 microns. We report that neurons transport fluorescently labeled silica microspheres with diameters of 1–2 microns into neurons in vitro and in rat brain without having overt effects on cell viability. Using flow cytometry, fluorescence activated cell sorting (FACS), and confocal and electron microscopy, we first found that SH-SY5Y human neuroblastoma cells internalized 1-micron silicon microspheres with surface charges of −70 mV (hydroxyl and carboxyl), −30 mV (amino) and +40 mV (ammonio). Uptake was rapid, within 2–4 hours, and did not affect cell viability 48 hours later. Flow cytometry assays indicate that SH-SY5Y cells internalize 1 and 1.5-micron microspheres at the same rate over a 24-hour incubation period. Electron microscopy confirms that SH-SY5Y cells internalize 1, 1.5 and 2-micron microspheres. Confocal microscopy demonstrated that primary cortical neurons (PCNs) also internalized 1, 1.5 and 2-micron amino microspheres within 4 hours. Finally, we injected 1-micron amino microspheres into rat striatum and found microspheres inside neurons. Overall, neurons can internalize microspheres up to 2 microns in diameter with a range of surface chemical groups and charges. These findings allow a host of neuroscience and neuroengineering applications including intracellular microdevices within neurons.

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In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells

Cited by 17 publications

References 39 publications

<p>The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies</p>

<p>The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies</p>

Effect of surface organic coatings of cellulose nanocrystals on the viability of mammalian cell lines

Neurons Internalize Functionalized Micron-Sized Silicon Dioxide Microspheres

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