In vitro cytotoxicity of superheated steam hydrolyzed oligo((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) and characteristics of its blend with poly(L-lactic acid) for biomaterial applications

Abstract

In order to clarify the in vitro cytotoxicity effect of superheated steam (SHS) treated poly((R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate) (PHBHHx) for biomaterial applications, SHS-treated PHBHHx oligoester samples: P(HB-co-6%-HHx) and P(HB-co-11%-HHx) with low and high percentages of unsaturated chain ends were evaluated for their cytotoxicity effects toward the growth of mouse fibroblast cell line NIH 3T3. From the results obtained after 24 and 48 h of the growth test, the SHS-treated PHBHHx oligoesters were found to be nontoxic to the growth of mouse fibroblast NIH 3T3 cell line with cell viability percentages of more than 95%. In order to serve as a potential resorbable medical suture, PHBHHx oligoesters were blended with poly(L-lactic acid) (PLLA) with a weight ratio of PHBHHx oligoester/PLLA = 20:80 (wt/wt) to improve mechanical properties of PHBHHx oligoesters. The PHBHHx oligoesters/PLLA blend films were evaluated for their thermal, mechanical, and surface wetting properties. Thermal properties of the blend films suggested a good compatibility between PHBHHx oligoesters and PLLA components. Mechanical properties of the blend films were determined to be close enough to a desirable strength range of medical sutures. Moreover, contact angle range of 65 < θ < 70° for the blend samples could provide desirable cell adhesion when used as biomaterials. Therefore, the blend of SHS-treated PHBHHx oligoesters and PLLA would be an ideal choice to be used as biomedical materials.

Physicochemical and Biological Characterisation of Diclofenac Oligomeric Poly(3-hydroxyoctanoate) Hybrids as β-TCP Ceramics Modifiers for Bone Tissue Regeneration

“…For these reasons, we have carried out direct compatibility analysis, seeding the (MC3T3-E1) directly on our materials ( Figure 7 B,C). Cell proliferation ability on surfaces based on PHAs was evaluated already in various cell models [ 25 , 26 , 27 , 31 , 32 , 33 , 34 , 59 ]. Our study shows increased viability along with the time of cell incubation on the surface of TCP/P(3HO) composites.…”

“…The use of a low molecular weight matrix is also beneficial in terms of increasing the degradation rate of the polymeric material as shown in [ 64 ]. Rajaratanam et al reported that the use of (P(3HB)-co-(3HHx)) oligoesters favours the process of the degradation of threads made of these materials [ 27 ].…”

“…In recent years, these compounds were the subject of interest of scientists involved in the production of materials for medical applications [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. Moreover, the attention of many researchers was focused on the chemical modification of PHAs and their copolymers in order to reach a stable chemical bond between the active substance and the matrix of the polymer or oligomer.…”

Biomedical Applications of Polyhydroxyalkanoate in Tissue Engineering

“…P(4HB) homopolymer has been discovered to be a promising material for absorbable sutures because its breakdown products are far less acidic than poly(glycolic acid) (PGA) or polycaprolactone (PCL), and it breaks down faster than PCL, PLLA and other PHAs, such as PHB [ 97 ]. By 2007, two other PHA-based suture materials–Phantom Fiber TM (Tornier Co., Bloomington, ID, USA) and MonoMax ® (Braun Surgical Co., Hessen, Germany)—had been FDA-approved and were prepared using P(4HB) [ 91 ].…”

Studies on Chemical IR Images of Poly(hydroxybutyrate–co–hydroxyhexanoate)/Poly(ethylene glycol) Blends and Two-Dimensional Correlation Spectroscopy

“…However, thermal, mechanical and chemical properties of PHB homopolymer by itself are somewhat limited for wider applications. To improve the properties of PHB, many attempts have been studied by copolymerization [2,8,9,10,11,12] and blending [13,14,15] with the other polymers. The PHB-based copolymers have been extensively studied using infrared (IR) [16,17,18,19] and Raman [8,10,20] spectroscopies, wide-angle X-ray scattering (WAXS) [11,20,21], X-ray diffraction (XRD) [22,23], differential scanning calorimetry (DSC) [24,25], and so on.…”