In Vitro Cytotoxicity Screening as a Criterion for the Rational Selection of Tear Substitutes

Abstract

A large number of artificial tears are currently available in the pharmaceutical market. Selecting the right drug for the patient remains a challenge for both the doctor and the patient. Comparing the cytotoxicity of artificial tears is one of the criteria for the rational selection of a drug that promotes maximum clinical efficacy and a higher safety profile. It is known that cells grown in vitro retain many metabolic features of the parent host tissues and at the same time lack tissue and organ interrelations and regulatory effects of the nervous and endocrine systems and have very limited compensatory capabilities. These features of cell cultures provide an opportunity to investigate the interaction of chemical agents directly with the cell itself, to identify changes in cellular and subcellular structures that can be masked in wholeorganism settings. This study presents the results of assessing the cytotoxicity of tear substitutes, which demonstrate that these drugs can have a cytostatic effect in vitro and differ in their cytotoxic potential. In recent years, the problem of drug therapy of patients with dry eye syndrome has been attracting increasing attention of ophthalmologists, so screening the cytotoxicity of a wide range of tear substitutes using cell culture-based test systems can promote the rational selection of these drugs.

Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency

“…Accompanying therapeutic agents, such as anesthetics, antibiotics, and anti-inflammatory drugs used during surgical manipulations and in the postoperative period, may also contribute negatively to the survivability of transplanted cells. The toxic effect of ophthalmic medications has been shown in vitro [ 43 , 44 ]. Assessing the toxic impact in vivo is still challenging.…”