In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells

Ali, M. Ajmal; Farah, Mohammad Abul; Al-Hemaid, Fahad M.A.; Abou-Tarboush, Faisal M.

doi:10.4238/2014.may.23.9

Cited by 26 publications

(19 citation statements)

References 32 publications

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“…In this study, the methanolic leaf extract of M. pseudolavatera Webb & Berthel. was examined for its anti-proliferative and pro-apoptotic effects on AML cell lines since leaves from other Malva species have shown cytotoxic effects on other cancer types [12][13][14]. In a previous study by Solowey et al (2014), the ethanolic extract from Urtica membranacea showed potent anti-cancer effects at 750 µg/mL and 1500 µg/mL and these concentrations showed to be therapeutically correlated to a mouse breast cancer model with no side-effects [29].…”

Section: Discussionmentioning

confidence: 99%

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Khoury

Haykal

Hodroj

et al. 2020

Cancers

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Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in the human diet due to its various curative effects. Many plant leaf extracts from the various species of Malva genus have been reported to possess anti-cancer properties, however, studies on M. pseudolavatera Webb & Berthel. leaves have documented anti-inflammatory and anti-oxidant effects with no emphasis on their possible anti-cancer potential. The present study explores the anti-cancer properties of Malva pseudolavatera Webb & Berthel. leaf extract on acute myeloid leukemia (AML) cell lines in vitro and deciphers the underlying molecular mechanism. Treatment of AML cell lines with M. pseudolavatera methanolic leaf extract showed a dose- and time-dependent inhibition of proliferation and a dose-dependent increase in apoptotic hallmarks such as an increase in phosphatidylserine on the outer membrane leaflet and membrane leakage in addition to DNA fragmentation. The pro-apoptotic effect was induced by reactive oxygen species (ROS) as well as an upregulation of cleaved poly(ADP-ribose) polymerase (PARP), increase in Bax/Bcl-2 ratio, andrelease of cytochrome-c from the mitochondria. Major compounds of the extract included methyl linolenate, phytol, γ-sitosterol, and stigmasterol as revealed by gas chromatography coupled with mass spectrometry, and amino acids, amino acid derivatives, tiliroside, 13-hydroxyperoxyoctadecadienoic, and quercitrin as detected by liquid chromatography coupled to mass spectrometry.

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Section: Discussionmentioning

confidence: 99%

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Khoury

Haykal

Hodroj

et al. 2020

Cancers

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“…Natural products possess immense pharmacological significance in the development of drugs including cancer (Kuete et al, 2013;Alil et al, 2014). Natural products with potential bioactivity compounds have received major attention of many researchers.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and histological evidences for the antitumor potential of <i>Teucrium Oliverianum</i> and <i>Rhazya stricta</i> in chemically-induced hepatocellular carcinoma

Shahat¹,

Alsaid²,

Kotob³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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Background: Teucrium oliverianum and Rhazya stricta are medicinal plants used in traditional and herbal medicine for the treatment of diabetes, liver diseases and inflammatory conditions. The present study was planned to investigate the antitumor efficacy of Teucrium oliverianum and Rhazya stricta in chemically-induced hepatocellular carcinoma (HCC) in rats. Materials and Methods: Forty adult male rats weighing 170-200 g were divided into four groups; each group was comprised of ten rats: (1): Normal healthy animals served as negative control group, (2): Hepatocellular carcinoma (HCC) group in which the rats were orally administered Nnitrosodiethylamine (dissolved in 0.9% normal saline), in a dose of 20 mg/kg b.wt. five times a week for six weeks, (3): HCC group treated with Teucrium oliverianum extract in a dose of 600 mg/kg b.wt for two months and (4): HCC group treated with Rhazya stricta extract in a dose of 750 mg/kg b.wt for two months. Serum alanine aminotransferase (ALT), asparatate aminotransferase (AST), alkaline phosphatase (ALP) and gammaglutamyl transferase (γ-GT) activities were estimated. Serum carcinoembyronic antigen (CEA), alpha-fetoprotein (AFP), alpha-L-fucosidase (AFU), glypican-3 (GPC-3), golgi protein 73 (Gp-73) and vascular endothelial growth factor (VEGF) levels were determined. Histopathological examination of liver tissue sections was also carried out. Results: The HCC group showed significant elevation in serum AST, ALT, ALP and γ-GT activities as well as CEA, AFP, AFU, Gpc-3, Gp 73 and VEGF levels versus the negative control group. Photomicrograph of liver tissue sections of rats in HCC revealed hepatic parenchyma with foci of anaplastic hepatocellular carcinoma as well as other foci of cystic cholangio carcinoma associated with areas of telangictasis with haemorrhage as well as individual hepatocellular necrosis. Conclusion: Treatment of HCC groups with Teucrium oliverianum or Rhazya stricta extract experienced significant improvement in the measured biochemical parameters as well as in the structural organization of the liver. In conclusion, the current study provided experimental evidences for the antitumor efficacy of Teucrium oliverianum and Rhazya stricta against hepatocellular carcinoma. Such effect could be attributed to hepatoprotective properties, antiproliferative activity and antiangiogenic potential.

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“…The plants screened in this this study (or plants of the same genus) have been previously reported to cause cytotoxic effects towards tumour cells and constitute therefore interesting candidates in a plant bioprospecting program for anticancer activity. The extract of L. dentata possess cytotoxic and pro-apoptotic properties assessed against human breast adenocarcinoma (MCF-7) cell lines using the MTT assay (Ali et al 2014). Ku et al (2014) evaluated antioxidant, antihyperglycemic, anti-cancer, anti-inflammatory, and anti-coagulant activities of hyperoside, an active compound from the genera of Crataegus and Hypericum.…”

Section: Antiproliferative Propertiesmentioning

confidence: 99%

Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

Nunes

Paśko

Tyszka-Czochara

et al. 2016

Pharmaceutical Biology

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Context: Crataegus monogyna L. (Rosaceae) (CM), Equisetum telmateia L. (Equisataceae) (ET), Geranium purpureum Vil. (Geraniaceae) (GP), Mentha suaveolens Ehrh. (Lamiaceae) (MS), and Lavandula stoechas L. spp. luisieri (Lamiaceae) (LS) are all medicinal. Objective: To evaluate the antioxidant, antiproliferative and antimicrobial activities of plant extracts and quantify individual phenolics and zinc. Material and methods: Aerial part extracts were prepared with water (W), ethanol (E) and an 80% mixture (80EW). Antioxidant activity was measured with TAA, FRAP and RP methods. Phenolics were quantified with a HPLC. Zinc was quantified using voltammetry. Antibacterial activity (after 48 h) was tested using Enterococcus faecalis, Bacillus cereus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes. Antiproliferative activity (after 24 h) was tested using HEP G2 cells and fibroblasts. Results: Solvents influenced results; the best were E and 80EW. GP had the highest antioxidant activity (TAA and FRAP of 536.90 mg AAE/g dw and 783.48 mg TE/g dw, respectively). CM had the highest zinc concentration (37.21 mg/kg) and phenolic variety, with neochlorogenic acid as the most abundant (92.91 mg/100 g dw). LS was rich in rosmarinic acid (301.71 mg/100 g dw). GP and LS inhibited the most microorganisms: B. cereus, E. coli and S. aureus. GP also inhibited E. faecalis. CM had the lowest MIC: 5830 lg/mL. The antibacterial activity is explained by the phenolics present. LS and CM showed the most significant anti-proliferative activity, which is explained by their zinc content. Conclusion: The most promising plants for further studies are CM, LS and GP.ARTICLE HISTORY

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In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells

Cited by 26 publications

References 32 publications

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Malva pseudolavatera Leaf Extract Promotes ROS Induction Leading to Apoptosis in Acute Myeloid Leukemia Cells In Vitro

Biochemical and histological evidences for the antitumor potential of <i>Teucrium Oliverianum</i> and <i>Rhazya stricta</i> in chemically-induced hepatocellular carcinoma

Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

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