In vitro cytotoxicity studies of surface modified CaS nanoparticles on L929 cell lines using MTT assay

Rekha, S.; Anila, E.I.

doi:10.1016/j.matlet.2018.11.009

Cited by 58 publications

(24 citation statements)

References 10 publications

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“…When HT-29 cells were treated with WBPs, such as LP-NK2 WBP, LPP-YK1 WBP, LP-YK1 WBP, and LF-NK1 WBP, the cell viabilities were > 90% ( Fig 1 ), which is appropriate because the value was more than the 70% cut-off, as recommended by ISO 10993–5: 2009 (Biological Evaluation of Medical Devices part 5: Tests for in vitro Cytotoxicity), indicating that WBPs were biocompatible [ 15 ]. However, the cell viabilities of PP-NK1 WBP and LP-NK1 WBP were less than 60%.…”

Section: Resultsmentioning

confidence: 99%

Development of postbiotics by bioconverting whey using Lactobacillus plantarum SMFM2017-YK1 and Limosilactobacillus fermentum SMFM2017-NK1 to alleviate periodontitis

et al. 2022

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This study investigated the effects of whey bioconversion products (WBPs) produced by lactic acid bacteria on periodontal disease. WBPs were prepared by fermenting whey with seven lactic acid bacteria, Limosilactobacillus fermentum SMFM2017-CK1 (LF-CK1), L. plantarum SMFM2017-NK2 (LP-NK2), Pediococcus pentosaceus SMFM2017-NK1 (PP-NK1), L. plantarum SMFM2017-NK1 (LP-NK1), L. paraplantarum SMFM2017-YK1 (LPP-YK1), L. plantarum SMFM2017-YK1 (LP-YK1), and L. fermentum SMFM2017-NK1 (LF-NK1)]; the pH of the fermented whey was adjusted to 6.5, followed by centrifugation. WBPs were examined for their effect on cell viability and antimicrobial activity against periodontal pathogens. The selected WBPs were used in animal experiments. After inducing periodontitis through right mandibular first molar ligation, WBPs were administered orally for 8 weeks. After sacrifice, gene and protein expression analyses of genes related to inflammatory and oxidative stress were performed, and histopathological analysis of gingival tissue was conducted. Our results showed that LP-YK1 WBP (WBP produced by LP-YK1) and LF-NK1 WBP (WBP produced by LF-NK1) groups exerted higher anti-inflammatory and antioxidant effects compared to the control group (p < 0.05). Histopathological analysis revealed that infiltration of inflammatory cells and epithelial cell proliferation were reduced in the LP-YK1 WBP group. These results indicate that WBPs prepared with LP-YK1 can be used as a postbiotic to alleviate periodontitis.

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Section: Resultsmentioning

confidence: 99%

Development of postbiotics by bioconverting whey using Lactobacillus plantarum SMFM2017-YK1 and Limosilactobacillus fermentum SMFM2017-NK1 to alleviate periodontitis

et al. 2022

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“…Generally, MTT is a way of investigating the toxicity of materials and their effect on cell viability 52 . In this study, the MTT assay was used to assess the effect of fibrous scaffolds on the viability of both osteoblast‐like (SAOS‐2) and fibroblasts (L929) cells in 1, 3, and 7 days (Figure 9).…”

Section: Resultsmentioning

confidence: 99%

“…Generally, MTT is a way of investigating the toxicity of materials and their effect on cell viability. 52 In this study, the MTT assay was used to assess the effect of fibrous scaffolds on the viability of both osteoblast-like (SAOS-2) and fibroblasts (L929) cells in 1, 3, and 7 days (Figure 9). Figure 9(a) shows that all samples had a steady trend up to 7 days and S1 represented ≥70% cell viability, which based on International Organization for Standardization (ISO) norm was acceptable cell viability on the GTR side.…”

Section: Mtt Assaymentioning

confidence: 99%

Novel bilayer electrospun poly(caprolactone)/ silk fibroin/ strontium carbonate fibrous nanocomposite membrane for guided bone regeneration

Etemadi

Mehdikhani

Poorazizi

et al. 2020

J of Applied Polymer Sci

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In this study, a thin layer with a thickness of about 120 μm of poly(caprolactone) (PCL) was fabricated by electrospinning method. Then, a fibrous nanocomposite composed of PCL/silk fibroin/strontium carbonate (PCL/SF/SrCO3) was electrospun on the prepared layer. Then, they were characterized. The mechanical properties, water uptake, degradation rate, wettability, porosity, and bioactivity of the electrospun membrane were scrutinized in vitro. Cytotoxicity of the samples was assessed by using osteoblast‐like cells (SAOS‐2) and L929 fibroblasts. Moreover, the cell adhesion, alkaline phosphatase (ALP) activity, and calcium deposition through alizarin red staining were conducted. Results revealed that the bilayer structure doubled the optimum mechanical properties and the addition of SrCO3 up to 15%–20% increased ALP activity, calcium deposition, and bioactivity. According to the results, the nanofibrous bilayer membrane containing 20 wt% SrCO3, 20 wt% SF, and 60 wt% PCL was chosen as the optimum sample. Therefore, this membrane could be applied in guided bone regeneration (GBR).

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“…Rekha and Anila [8] showed the in vitro cytotoxicity of Triethanolamine-coated CaS nanoparticles in human fibroblast cell lines by the MTT assay. In their study, cell viability was greater than 90% at concentrations ranging from 6.25 to 100 µg/mL.…”

Section: Discussionmentioning

confidence: 99%

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

Nepomuceno

Carvalho

Soares

et al. 2020

Preprint

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Background Aiming to develop essential chemotherapeutic drugs to control various types of cancer, one of the primary tests is the tetrazolium reduction method. The present study aims to standardize the electromagnetic spectrum that best discriminates the absorbance and to evaluate the drug dosage of single and daily doses during the cell viability and toxicity analysis by the tetrazolium reduction method, using the ethanol extract of pequi peel in canine osteosarcoma cells. Results The wavelength of 532 nm showed the best results. We found that as the treatment time increased, the formazan conversion reduced. After 72 hours of treatment, we observed clear discrimination of dose-dependent data, with up to five discriminants within 72 hours with a change in absorbance from 0.554 to 0.064 A. The wavelength of 570 nm it was not ideal since we could not discern the difference in the spectral reflectance of the treatments and, therefore, show no statistical difference among treatments. We found no statistical difference for the coefficient of variation at wavelengths of 532 and 570nm, which were 12.77% and 8.80% respectively. Conclusions The wavelength of 532nm best discriminated the absorbance, as it presented better ability to congregate the treatment groups, greater variation between the discriminant and lower coefficient of variation, during the colorimetric test to evaluate the cellular metabolism. Moreover, the ethanol extract of pequi peel in canine osteosarcoma cells showed a statistically equal effect of a single dose administration to the dose reapplied every 24 hours.

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In vitro cytotoxicity studies of surface modified CaS nanoparticles on L929 cell lines using MTT assay

Cited by 58 publications

References 10 publications

Development of postbiotics by bioconverting whey using Lactobacillus plantarum SMFM2017-YK1 and Limosilactobacillus fermentum SMFM2017-NK1 to alleviate periodontitis

Development of postbiotics by bioconverting whey using Lactobacillus plantarum SMFM2017-YK1 and Limosilactobacillus fermentum SMFM2017-NK1 to alleviate periodontitis

Novel bilayer electrospun poly(caprolactone)/ silk fibroin/ strontium carbonate fibrous nanocomposite membrane for guided bone regeneration

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

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