In-Vitro Cytotoxicity Study: Cell Viability and Cell Morphology of Carbon Nanofibrous Scaffold/Hydroxyapatite Nanocomposites

Abstract

Electrospun carbon nanofibers (CNFs), which were modified with hydroxyapatite, were fabricated to be used as a substrate for bone cell proliferation. The CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers after two steps of heat treatment: stabilization and carbonization. Carbon nanofibrous (CNF)/hydroxyapatite (HA) nanocomposites were prepared by two different methods; one of them being modification during electrospinning (CNF-8HA) and the second method being hydrothermal modification after carbonization (CNF-8HA; hydrothermally) to be used as a platform for bone tissue engineering. The biological investigations were performed using in-vitro cell counting, WST cell viability and cell morphology after three and seven days. L929 mouse fibroblasts were found to be more viable on the hydrothermally-modified CNF scaffolds than on the unmodified CNF scaffolds. The biological characterizations of the synthesized CNF/HA nanofibrous composites indicated higher capability of bone regeneration.

The Effect of Liquid Phase Concentration on the Setting Time and Compressive Strength of Hydroxyapatite/Bioglass Composite Cement

“…Additionally, the results of immersion into SBF solution revealed that most changes in the Ca/P ratio were found for the HAp70/BG30 sample, indicating the highest bioactivity of all samples. These results agree with previous research [46,47].…”

Quercetin-gold nanorods incorporated into nanofibers: development, optimization and cytotoxicity

“…The biocompatibility of nanobers was demonstrated in the literature towards several types of normal cells; recently, mouse broblast exhibited high viability upon treatment with electrospun carbon nanobers modied with hydroxyapatite. 73 Our results indicate the biocompatibility of GNR-containing nanobers, particularly those containing a low concentration of GNR.…”

“…The biocompatibility of nanofibers was demonstrated in the literature towards several types of normal cells; recently, mouse fibroblast exhibited high viability upon treatment with electrospun carbon nanofibers modified with hydroxyapatite. 73 …”

Electrospun non-wovens potential wound dressing material based on polyacrylonitrile/chicken feathers keratin nanofiber

“…Electrospun non-woven wound materials have been extensively studied. Because they can be designed to prevent bacterial invasion, promote cell proliferation, as well as adsorption of wound exudates and dehydration of the wound surface 8 , 9 . Furthermore, because of their microporous nature, they can act as the extracellular matrix (ECM) by promoting cell adhesion and migration 10 , 11 .…”

“…Electrospun non-woven wound materials have been extensively studied. Because they can be designed to prevent bacterial invasion, promote cell proliferation, as well as adsorption of wound exudates and dehydration of the wound surface 8,9 . Furthermore, because of their microporous nature, they can act as the extracellular matrix (ECM) by promoting cell adhesion and migration 10,11 .Nanofibers for wound dressing come in a variety of shapes and sizes, they can be prepared from a variety of polymers (natural or synthetic), or they are made by blinding more than one type.…”