In vitro degradability and bioactivity of oxidized bacterial cellulose-hydroxyapatite composites

Luz, Erika Patricia Chagas Gomes; Chaves, Paulo Hiago Silva; Vieira, Lídia de Araújo Pinto; Ribeiro, Sádwa Fernandes; Borges, M. de F.; Andrade, Fábia Karine; Muniz, Celli Rodrigues; Infantes‐Molina, Antonia; Rodríguez‐Castellón, Enrique; Rosa, Morsyleide de Freitas; Vieira, Melissa Gurgel Adeodato

doi:10.1016/j.carbpol.2020.116174

Cited by 51 publications

(16 citation statements)

References 34 publications

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“…Previous studies have enhanced the degradability of BC by oxidizing it. We have also improved biocompatibility and degradability by oxidizing and perforating it and binding it to soy protein for urethral repair [ 35 ]. In the present study, we sulfonated and perforated the B.C Compared to BC or single modified BC, DMBC retains its mechanical properties better, while its biocompatibility and degradability are significantly enhanced.…”

Section: Discussionmentioning

confidence: 99%

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

Zhu

Yang

et al. 2022

Stem Cell Res Ther

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Background Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology. The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem. Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity. Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification. Methods First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation. We tested the relevant mechanical properties of these materials. After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering. Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation. At the same time, we detected the potential biological function of FGFR2 by bioinformatics and a cytokine chip. Results The results show that the composite has excellent repairability and that this ability is correlated with angiogenesis. The new composite in this study provides new insight and therapeutic methods for urethral reconstruction. The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs. Conclusions Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.

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Section: Discussionmentioning

confidence: 99%

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

Zhu

Yang

et al. 2022

Stem Cell Res Ther

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“…Different synthetic polymers (e.g., poly (vinyl alcohol) [73], carboxymethyl cellulose) [74], natural polymers (e.g., gelatin [75,76], alginate [77,78]), nanomaterials (e.g., hydroxyapatite (HAp) [79,80], bioactive glass (BG) [81,82], carbon nanotubes (CNTs) [83], graphene oxide (GO) [84]), proteins (e.g., collagen [85]), amino acid sequences (e.g., RGD 3366) with a bath-system (BioPuls) for uniaxial in aqua tension (d), schematic of getting effective elastic modulus of bulk hydrogel (e), custom-made attachment to fix stretched specimens to prevent elastic recovery (f), digital cameras (two) to evaluate changes in the geometry of specimens (g), and optical microscopical images of freeze-dried specimens to measure total volume (h). Reproduced with permission from [71].…”

Section: Surface Modification Of Bacterial Nanocellulosementioning

confidence: 99%

“…Different synthetic polymers (e.g., poly (vinyl alcohol) [73], carboxymethyl cellulose) [74], natural polymers (e.g., gelatin [75,76], alginate [77,78]), nanomaterials (e.g., hydroxyapatite (HAp) [79,80], bioactive glass (BG) [81,82], carbon nanotubes (CNTs) [83], graphene oxide (GO) [84]), proteins (e.g., collagen [85]), amino acid sequences (e.g., RGD [86]), biomolecules (e.g., growth factors [87]), antifungals (propolis [52]), antioxidants (e.g., propolis [52], fisetin [88]), anti-inflammatory (propolis [52]), and antimicrobial agents (e.g., AgNPs, TiO 2 ) can be integrated with BNC by various strategies, using coating, gas plasma (e.g., nitrogen, oxygen) or irradiation (e.g., gamma) treatments, and surface sulfation or phosphorylation or other physical/chemical treatments to make BNC or BNCbased biomaterials more active as per desired applications [20]. Here, plasma techniques are effective strategies to change the BNC surface and optimize the biofunctionality without affecting native features [89].…”

Section: Surface Modification Of Bacterial Nanocellulosementioning

confidence: 99%

“…Therefore, to manipulate degradation behavior, Luz et al 2020 prepared oxidized-BNC (o-BNC) by periodate-oxidation for different time periods and developed BNC/HAp or o-BNC/HAp membranes through mineralization. o-BNC/HAp composite scaffold exhibited better bioactivity and degradability than that of BNC/HAp and was dependent on the degree of BNC oxidation, and thereby, it showed a high level of glucose and other by-products (e.g., butyric acid and acidic acid) [79]. Similarly, the same As nanocelluloses (e.g., CNFs) have been considered very effective in fabricating BG-based composite with enhanced osteoconductivity and mechanical properties [114].…”

Section: Bacterial Nanocellulose/filler-based Biomaterialsmentioning

confidence: 99%

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Efficacy of Bacterial Nanocellulose in Hard Tissue Regeneration: A Review

Kumar

Han

2021

Materials

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Bacterial nanocellulose (BNC, as exopolysaccharide) synthesized by some specific bacteria strains is a fascinating biopolymer composed of the three-dimensional pure cellulosic nanofibrous matrix without containing lignin, hemicellulose, pectin, and other impurities as in plant-based cellulose. Due to its excellent biocompatibility (in vitro and in vivo), high water-holding capacity, flexibility, high mechanical properties, and a large number of hydroxyl groups that are most similar characteristics of native tissues, BNC has shown great potential in tissue engineering applications. This review focuses on and discusses the efficacy of BNC- or BNC-based biomaterials for hard tissue regeneration. In this review, we provide brief information on the key aspects of synthesis and properties of BNC, including solubility, biodegradability, thermal stability, antimicrobial ability, toxicity, and cellular response. Further, modification approaches are discussed briefly to improve the properties of BNC or BNC-based structures. In addition, various biomaterials by using BNC (as sacrificial template or matrix) or BNC in conjugation with polymers and/or fillers are reviewed and discussed for dental and bone tissue engineering applications. Moreover, the conclusion with perspective for future research directions of using BNC for hard tissue regeneration is briefly discussed.

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“…The size of the cHAp clusters varies over a wide range (1-15 µm). There are many attempts to prepare hydroxyapatite-biopolymer composites with appropriate bioactive properties for biomedical use or for bone tissue engineering; however, the majority of them use poly(l-lactic acid) (PLLA), poly(e-caprolactone), chitosan, and gelatines matrices [60][61][62][63][64][65][66][67], and only a few of them apply PVP or CA [68][69][70]. There are many attempts to prepare hydroxyapatite-biopolymer composites with appropriate bioactive properties for biomedical use or for bone tissue engineering; however, the majority of them use poly(l-lactic acid) (PLLA), poly(e-caprolactone), chitosan, and gelatines matrices [60][61][62][63][64][65][66][67], and only a few of them apply PVP or CA [68][69][70].…”

Section: Morphological Characterisation Of Pure and Chap-loaded Pvp And Camentioning

confidence: 99%

Comparison of the Morphological and Structural Characteristic of Bioresorbable and Biocompatible Hydroxyapatite-Loaded Biopolymer Composites

et al. 2021

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Calcium phosphate (CaP)-based ceramic–biopolymer composites can be regarded as innovative bioresorbable coatings for load-bearing implants that can promote the osseointegration process. The carbonated hydroxyapatite (cHAp) phase is the most suitable CaP form, since it has the highest similarity to the mineral phase in human bones. In this paper, we investigated the effect of wet chemical preparation parameters on the formation of different CaP phases and compared their morphological and structural characteristics. The results revealed that the shape and crystallinity of CaP particles were strongly dependent on the post-treatment methods, such as heat or alkaline treatment of as-precipitated powders. In the next step, the optimised cHAp particles have been embedded into two types of biopolymers, such as polyvinyl pyrrolidone (PVP) and cellulose acetate (CA). The pure polymer fibres and the cHAp–biopolymer composites were produced using a novel electrospinning technique. The SEM images showed the differences between the morphology and network of CA and PVP fibres as well as proved the successful attachment of cHAp particles. In both cases, the fibres were partially covered with cHAp clusters. The SEM measurements on samples after one week of immersion in PBS solution evidenced the biodegradability of the cHAp–biopolymer composites.

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In vitro degradability and bioactivity of oxidized bacterial cellulose-hydroxyapatite composites

Cited by 51 publications

References 34 publications

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

Efficacy of Bacterial Nanocellulose in Hard Tissue Regeneration: A Review

Comparison of the Morphological and Structural Characteristic of Bioresorbable and Biocompatible Hydroxyapatite-Loaded Biopolymer Composites

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