In vitro degradation profiles and in vivo biomaterial–tissue interactions of microwell array delivery devices

Hadavi, Elahe; Vries, Rick de; Smink, Alexandra M.; Haan, Bart de; Leijten, Jeroen; Schwab, Leendert W.; Karperien, Marcel; Vos, Paul de; Dijkstra, Pieter J.; Apeldoorn, Aart van

doi:10.1002/jbm.b.34686

Cited by 7 publications

(10 citation statements)

References 43 publications

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“…Either in cattle or sheep CE cysts, the presence of a granulomatous reaction is indicative of a foreign body response, which has been widely studied in the biomaterials field [ 25 – 27 ], where after an acute inflammatory reaction, there is chronic inflammation, followed by either granulomatous tissue or the development of a fibrous capsule [ 27 ]. Here we report that in non-fertile CE cysts, this fibrous capsule changes its location; in cattle it is found surrounding the chronic inflammation tissue, whereas in sheep is found directly surrounding the laminated layer.…”

Section: Discussionmentioning

confidence: 99%

Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep

Hidalgo

Stoore

Baquedano

et al. 2021

Vet Res

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Cystic echinococcosis is a zoonotic disease caused by the metacestode of Echinococcus granulosus sensu lato. The disease is characterized by the development of cystic structures inside viscera of the intermediate host, mainly liver and lungs. These cysts are formed by three layers: germinal, laminated, and adventitial layer, the latter being the local host immune response. Metacestodes that develop protoscoleces, the infective stage to the definitive host, are termed fertile, whereas cysts that do not produce protoscoleces are termed non-fertile. Sheep usually harbor fertile cysts while cattle usually harbor non-fertile cysts. Adventitial layers with fibrotic resolution are associated to fertile cysts, whereas a granulomatous reaction is associated with non-fertile cysts. The aim of this study was to analyze cellular distribution in the adventitial layer of fertile and non-fertile E. granulosus sensu stricto cysts found in liver and lungs of cattle and sheep. A total of 418 cysts were analyzed, 203 from cattle (8 fertile and 195 non-fertile) and 215 from sheep (64 fertile and 151 non-fertile). Fertile cysts from cattle showed mixed patterns of response, with fibrotic resolution and presence of granulomatous response in direct contact with the laminated layer, while sheep fertile cysts always displayed fibrotic resolution next to the laminated layer. Cattle non-fertile cysts display a granulomatous reaction in direct contact with the laminated layer, whereas sheep non-fertile cysts display a granulomatous reaction, but in direct contact with the fibrotic resolution. This shows that cattle and sheep cystic echinococcosis cysts have distinct local immune response patterns, which are associated to metacestode fertility.

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Section: Discussionmentioning

confidence: 99%

Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep

Hidalgo

Stoore

Baquedano

et al. 2021

Vet Res

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“…A similar medium at various pH was applied to assess the degradation profile of the PEOT-PBT and poly(ester urethane) films [11]. Bremrer et al [12] used the guide of [13] for the evaluation of the degradation in vitro potentiality of the poly(ester)urethane-based adhesive.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Advanced Cranial and Craniofacial Implant Fabrication on Their Degradation Affinity

Chmal-Fudali,

Basińska,

Kucharska-Jastrząbek

et al. 2023

Materials

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Biodegradable craniofacial and cranial implants are a new aspect in terms of reducing potential complications, especially in the long term after surgery. They are also an important contribution in the field of surgical reconstructions for children, for whom it is important to restore natural bone in a relatively short time, due to the continuous growth of bones. The aim of this study was to verify the impact of the technology on biodegradability and to estimate the risk of inappropriate implant resorption time, which is an important aspect necessary to select prototypes of implants for in vivo testing. Prototypes of implants were made using two technologies: 3D printing using a PLDLA: poly(L-co-D,L lactide) (PLDLA) filament containing hydroxyapatite nanoparticles, and injection using PLDLA. After the radiation sterilization process, they were subjected to in vitro degradation under accelerated conditions. As part of this study, the in vitro degradation of newly developed biodegradable implant technologies was assessed in accordance with the guidelines of European standards. It was found that the implant manufacturing process had a significant impact on the degradation time under simulated conditions in various media. Implants made using the injection technique were characterized by lower susceptibility to degradation media compared to the 3D-printed implant under accelerated conditions.

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“…The primary challenge in developing biodegradable hydrogels for drug delivery lies in accurately measuring their degradation over time, while also simultaneously being able to evaluate the drug-release kinetics. Standard approaches to assess the biodegradation of hydrogels are either by volume or mass loss, the latter of which is more common [13][14][15]. When assessing mass loss, researchers measure the weight of the dried gel at a specified time point [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…Standard approaches to assess the biodegradation of hydrogels are either by volume or mass loss, the latter of which is more common [13][14][15]. When assessing mass loss, researchers measure the weight of the dried gel at a specified time point [14,15]. This procedure is exceptionally cumbersome and time consuming because a dried gel from one time point cannot be reused for subsequent measurements.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Screening Platform for Simultaneous Evaluation of Biodegradation and Therapeutic Release of an Ocular Hydrogel

Ho,

Phan,

Garg

et al. 2023

Pharmaceutics

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This study attempts to address the challenge of accurately measuring the degradation of biodegradable hydrogels, which are frequently employed in drug delivery for controlled and sustained release. The traditional method utilizes a mass-loss approach, which is cumbersome and time consuming. The aim of this study was to develop an innovative screening platform using a millifluidic device coupled with automated image analysis to measure the degradation of Gelatin methacrylate (GelMA) and the subsequent release of an entrapped wetting agent, polyvinyl alcohol (PVA). Gel samples were placed within circular wells on a custom millifluidic chip and stained with a red dye for enhanced visualization. A camera module captured time-lapse images of the gels throughout their degradation. An image-analysis algorithm was used to translate the image data into degradation rates. Simultaneously, the eluate from the chip was collected to quantify the amount of GelMA degraded and PVA released at various time points. The visual method was validated by comparing it with the mass-loss approach (R = 0.91), as well as the amount of GelMA eluted (R = 0.97). The degradation of the GelMA gels was also facilitated with matrix metalloproteinases 9. Notably, as the gels degraded, there was an increase in the amount of PVA released. Overall, these results support the use of the screening platform to assess hydrogel degradation and the subsequent release of entrapped therapeutic compounds.

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In vitro degradation profiles and in vivo biomaterial–tissue interactions of microwell array delivery devices

Cited by 7 publications

References 43 publications

Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep

Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep

Effect of the Advanced Cranial and Craniofacial Implant Fabrication on Their Degradation Affinity

A Rapid Screening Platform for Simultaneous Evaluation of Biodegradation and Therapeutic Release of an Ocular Hydrogel

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