In vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infections

Mendes, João João; Leandro, Clara; Mottola, Carla; Barbosa, Raquel; Silva, Filipa A.; Oliveira, Manuela; Vilela, C. L.; Melo‐Cristino, José; Górski, Andrzej; Pimentel, Madalena; São-José, Carlos; Cavaco‐Silva, Patrícia; Garcia, Miguel

doi:10.1099/jmm.0.071753-0

Cited by 57 publications

(38 citation statements)

References 53 publications

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“…aureus , P . aeruginosa and Acinetobacter baumannii strains from DFIs . This method, and other new techniques, is currently being explored in several countries.…”

Section: The ‘Post‐antibiotic’ Era?mentioning

confidence: 99%

“…In several Eastern European countries, however, they continue to be used with good results. A recent study from Portugal characterized five bacteriophages found to have antimicrobial activity against both planktonic and biofilm isolates of S. aureus, P. aeruginosa and Acinetobacter baumannii strains from DFIs [29]. This method, and other new techniques, is currently being explored in several countries.…”

Section: The Antibiotic Eramentioning

confidence: 99%

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Diabetic foot infections: Current treatment and delaying the ‘post‐antibiotic era’

Lipsky

2016

Diabetes Metabolism Res

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Background Treatment for diabetic foot infections requires properly diagnosing infection, obtaining an appropriate specimen for culture, assessing for any needed surgical procedures and selecting an empiric antibiotic regimen. Therapy will often need to be modified based on results of culture and sensitivity testing. Because of excessive and inappropriate use of antibiotics for treating diabetic foot infections, resistance to the usually employed bacteria has been increasing to alarming levels.

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“…aureus , P . aeruginosa and Acinetobacter baumannii strains from DFIs . This method, and other new techniques, is currently being explored in several countries.…”

Section: The ‘Post‐antibiotic’ Era?mentioning

confidence: 99%

Section: The Antibiotic Eramentioning

confidence: 99%

Diabetic foot infections: Current treatment and delaying the ‘post‐antibiotic era’

Lipsky

2016

Diabetes Metabolism Res

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“…Using metabolic activity as a measure of cell viability, it was observed that bacteriophage treatment significantly increased cell impairment within biofilms. Moreover, bacteriophage exposure repeated every four hours caused a further decrease in cell activity [9].…”

Section: Bacteriophagesmentioning

confidence: 99%

“…Diabetes-associated foot ulcer infections are predominantly polymicrobial and several bacterial genera can be part of the DFU microbiota, namely Staphylococcus, Pseudomonas, Streptococcus, Enterococcus, Corynebacterium, Acinetobacter, Prevotella, Porphyromonas, and members of the family Enterobacteriaceae. The predominant Gram-positive and Gram-negative species present in DFU are Staphylococcus aureus and Pseudomonas aeruginosa, respectively [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Biofilms in Diabetic Foot Ulcers: Potential Alternative Therapeutics

Santos¹,

Veiga²,

Tavares³

et al. 2016

Microbial Biofilms - Importance and Applications

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Diabetes mellitus is a major health problem that affects approximately million people globally. One of its most severe complications is the development of diabetic foot ulcers DFU . Ischemic and neurophatic lesions are of major importance for DFU onset however, it is the infection by multidrug-resistant and biofilm-producing microorganisms, along with local microenvironmental conditions unfavorable to antibiotics action that ultimately cause infection chronicity and lower limbs amputation. Novel therapeutic protocols for DFU management are extremely urgent. "acteriophages, probiotics and antimicrobial peptides "MP have recently been proposed as alternatives to currently available antibiotics. "acteriophages are viruses that specifically infect and multiply within bacterial cells. Their ability to diffuse through polymeric matrixes makes them particularly efficient to eradicate biofilmbased bacteria. Promising results were also observed with probiotic therapy. Probiotics are well-characterized strains with the ability to compete with pathogenic microorganisms and modulate the host immune response. "MP are molecules produced by living organisms as part of their innate immune response. Unlike conventional antibiotics, "MP also act as immunomodulators and resistance to "MP was rarely observed, supporting their potential as therapeutic agents. These innovative therapeutic strategies may in the future substitute or complement antibiotherapy, ultimately contributing for the decrease in multidrug-resistant bacteria dissemination.

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“…Multiple phages with different host ranges and targeting different receptors can be combined in a single phage preparation (phage cocktails) to expand their spectrum of activity and prevent the development of phage-resistant bacterial variants [70]. Several studies have reported the use of this strategy to treat bacterial biofilms [2,10,11,51,71]. For example, Fu et al studied the prevention of P. aeruginosa biofilm formation on hydrogelcoated catheters pre-treated with phages [10].…”

Section: Phage Cocktailsmentioning

confidence: 99%

Phage therapy as an alternative or complementary strategy to prevent and control biofilm-related infections

Pires

Melo

Boas

et al. 2017

Current Opinion in Microbiology

230

160

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The complex heterogeneous structure of biofilms confers to bacteria an important survival strategy. Biofilms are frequently involved in many chronic infections in consequence of their low susceptibility to antibiotics as well as resistance to host defences. The increasing need of novel and effective treatments to target these complex structures has led to a growing interest on bacteriophages (phages) as a strategy for biofilm control and prevention. Phages can be used alone, as a cocktail to broaden the spectra of activity, or in combination with other antimicrobials to improve their efficacy. Here, we summarize the studies involving the use of phages for the treatment or prevention of bacterial biofilms, highlighting the biofilm features that can be tackled with phages or combined therapy approaches.

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In vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infections

Cited by 57 publications

References 53 publications

Diabetic foot infections: Current treatment and delaying the ‘post‐antibiotic era’

Diabetic foot infections: Current treatment and delaying the ‘post‐antibiotic era’

Bacterial Biofilms in Diabetic Foot Ulcers: Potential Alternative Therapeutics

Phage therapy as an alternative or complementary strategy to prevent and control biofilm-related infections

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