In Vitro Detection of Hypoxia Using a Ratiometric Quantum Dot-Based Oxygen Sensor

Shamirian, Armen; Afsari, Hamid Samareh; Hassan, Asra; Miller, Leon K.; Snee, Preston T.

doi:10.1021/acssensors.6b00452

Cited by 33 publications

(19 citation statements)

References 53 publications

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“…The emission lifetimes of the nanoprobe also reduced from 4031 ns to 836 ns at O 2 concentrations of 0% to 21%, respectively. In vitro tests confirmed that this probe was capable of intracellular hypoxia imaging in HeLa cells [66]. …”

Section: Nanoparticle Based Probesmentioning

confidence: 97%

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Luminescent Probe Based Techniques for Hypoxia Imaging

Jaworski¹

2017

JNMR

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Hypoxia is a condition of tissue environments wherein a lower than normal level of oxygen is available, and it serves as the root cause and indicator of various diseases. Detection of hypoxia in tumors is imperative for furthering our understanding of the pathological effects and the development of proper treatments, as it is well established that hypoxic tumors are able to impede the cancer treatment process by being resistant to many therapies. It is important therefore to be able to detect hypoxia in tissues and tumors through in vivo imaging methods. A growing area for detection of hypoxia in vivo is the use of fluorescent/luminescent probes which has accelerated in recent years. The continued quest for improvements in selectivity and sensitivity has inspired researchers to pursue new strategies for fluorescence/luminescent probe design. This review will discuss various luminescent probes based on small molecules, dyes, macromolecules, and nanoparticles for sensitive and specific detection of oxygen levels directly or by indirect mechanisms such as the presence of enzymes or related factors that arise in a hypoxic environment. Following the particular mechanism of detection, each probe has specific structural and photophysical properties which permit its selectivity and sensitivity. These probes show promise in terms of low toxicity and high specificity among other merits discussed, and in providing new dimensions for hypoxia detection, these works contribute to future potential methods for clinical diagnosis of hypoxic tissues and tumors.

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Section: Nanoparticle Based Probesmentioning

confidence: 97%

“…(Figure reproduced from reference [66]; use permitted under the Creative Commons Attribution License CC BY 4.0. )…”

Section: Figurementioning

confidence: 99%

Luminescent Probe Based Techniques for Hypoxia Imaging

Jaworski¹

2017

JNMR

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“…Currently, these frequently-used self-referenced luminous nanoparticles mainly consist of dye-embedded silica, 153, 154 gold nanoclusters (AuNCs), 155–157 fluorescent bovine serum albumin nanoparticles, 158 Cdots, 159, 160 , silicon nanodots, 161 or quantum dots (QDs). 162, 163 Through a combination of the corresponding target-sensitive fluorescent dye units with unique responses to target analytes or molecular events, these dual-emission nanoparticle-dye nanoconjugates have been extensively applied for ratiometric sensing and imaging of diverse physical and physiological changes. For example, Tian et al applied Cdots as a reference unit and hydroethidine (HE) as a specific response unit to develop a dual emission nanoparticle-dye nanocomplex for ratiometric sensing and imaging of superoxide anion changes upon oxidative stress in cancer cells.…”

Section: Ratiometric Fluorescence Nanoprobesmentioning

confidence: 99%

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

et al. 2018

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Exploring and understanding biological and pathological changes are of great significance for early diagnosis and therapy of diseases. Optical sensing and imaging approaches have experienced major progress in this field. Particularly, an emergence of various functional optical nanoprobes has provided enhanced sensitivity, specificity, targeting ability, as well as multiplexing and multimodal capabilities due to improvements in their intrinsic physicochemical and optical properties. However, one of the biggest challenges of conventional optical nanoprobes is their absolute intensity-dependent signal readout, which causes inaccurate sensing and imaging results due to the presence of various analyte-independent factors that can cause fluctuations in their absolute signal intensity. Ratiometric measurements provide built-in self-calibration for signal correction, enabling more sensitive and reliable detection. Optimizing nanoprobe designs with ratiometric strategies can surmount many of the limitations encountered by traditional optical nanoprobes. This review first elaborates upon existing optical nanoprobes that exploit ratiometric measurements for improved sensing and imaging, including fluorescence, surface enhanced Raman scattering (SERS), and photoacoustic nanoprobes. Next, a thorough discussion is provided on design strategies for these nanoprobes, and their potential biomedical applications for targeting specific biomolecule populations (e.g. cancer biomarkers and small molecules with physiological relevance), for imaging the tumor microenvironment (e.g. pH, reactive oxygen species, hypoxia, enzyme and metal ions), as well as for intraoperative image guidance of tumor-resection procedures.

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“…Photoelectrochemical sensors using TQDs are based on change in photocurrent due to chemical changes by enzymes, QD-protein interactions and binding reactions between biomolecules [47]. Reported applications include photoelectrochemical detection of acetylcholine [48], oxygen [49], epinephrine [50], and hydrogen [51], [52] to name a few. Although the use of TQDs in a range of biosensing and bioimaging applications has been explored, their cytotoxicity has been a cause of concern.…”

Section: Carbon Nanotubes (Cnts)mentioning

confidence: 99%

Nanomaterials-Based Biosensors for COVID-19 Detection—A Review

et al. 2021

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This review paper discusses the properties of nanomaterials, namely graphene, molybdenum disulfide, carbon nanotubes, and quantum dots for unique sensing applications. Based on the specific analyte to be detected and the functionalization techniques that are employed, some noteworthy sensors that have been developed are discussed. Further, biocompatible sensors fabricated from these materials capable of detecting specific chemical compounds are also highlighted for COVID-19 detection purposes, which can aid in efficient and reliable sensing as well as timely diagnosis.

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In Vitro Detection of Hypoxia Using a Ratiometric Quantum Dot-Based Oxygen Sensor

Cited by 33 publications

References 53 publications

Luminescent Probe Based Techniques for Hypoxia Imaging

Luminescent Probe Based Techniques for Hypoxia Imaging

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

Nanomaterials-Based Biosensors for COVID-19 Detection—A Review

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